LOCUS       sequence-139448-        6883 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  expresses human myc-14-3-3-zeta in mammalian cells .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6883)
  AUTHORS   Chen HK, Fernandez-Funez P, Acevedo SF, Lam YC, Kaytor MD, Fernandez
            MH, Aitken A, Skoulakis EM, Orr HT, Botas J, Zoghbi HY
  TITLE     Interaction of Akt-phosphorylated ataxin-1 with 14-3-3 mediates 
            neurodegeneration in spinocerebellar ataxia type 1.
  JOURNAL   Cell. 2003 May 16;113(4):457-68.
  PUBMED    12757707
REFERENCE   2  (bases 1 to 6883)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell."; 
            date: "2003-05-16"; volume: "113(4)"; pages: "457-68"
COMMENT     Sequence Label: pcDNA3.1-Myc-14-3-3-zeta
FEATURES             Location/Qualifiers
     source          1..6883
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        16..395
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        396..607
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     558..578
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     604..628
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          768..900
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     883..902
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     promoter        945..963
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    join(1034..1608,1609..1611,1612..1620)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(1201..1218)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     1428..1447
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             2370..2399
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     polyA_signal    2465..2586
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2476..2495)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2530..2549
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      2772..3227
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2859..2878)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3069..3090
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        3344..3701
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        3344..3535
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(3367..3387)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      3552..3687
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             3752..4546
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3806..3825)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4416..4435
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    4610..4658
                     /note="synthetic polyadenylation signal"
     primer_bind     complement(4699..4718)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4818..4840
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(4878..4896)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4964..5068
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5069..5137,5138..5929)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5287..5306)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6100..6688
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6589..6608
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tatgaccgcc atgttggcat tgattattga ctagttatta atagtaatca attacggggt
       61 cattagttca tagcccatat atggagttcc gcgttacata acttacggta aatggcccgc
      121 ctggctgacc gcccaacgac ccccgcccat tgacgtcaat aatgacgtat gttcccatag
      181 taacgccaat agggactttc cattgacgtc aatgggtgga gtatttacgg taaactgccc
      241 acttggcagt acatcaagtg tatcatatgc caagtccgcc ccctattgac gtcaatgacg
      301 gtaaatggcc cgcctggcat tatgcccagt acatgacctt acgggacttt cctacttggc
      361 agtacatcta cgtattagtc atcgctatta ccatggtgat gcggttttgg cagtacacca
      421 atgggcgtgg atagcggttt gactcacggg gatttccaag tctccacccc attgacgtca
      481 atgggagttt gttttggcac caaaatcaac gggactttcc aaaatgtcgt aacaactgcg
      541 atcgcccgcc ccgttgacgc aaatgggcgg taggcgtgta cggtgggagg tctatataag
      601 cagagctcgt ttagtgaacc gtcagatcac tagaagcttt attgcggtag tttatcacag
      661 ttaaattgct aacgcagtca gtgcttctga cacaacagtc tcgaacttaa gctgcagtga
      721 ctctcttaag gtagccttgc agaagttggt cgtgaggcac tgggcaggta agtatcaagg
      781 ttacaagaca ggtttaagga gaccaataga aactgggctt gtcgagacag agaagactct
      841 tgcgtttctg ataggcacct attggtctta ctgacatcca ctttgccttt ctctccacag
      901 gtgtccactc ccagttcaat tacagctctt aaggctagag tacttaatac gactcactat
      961 aggctagcct cgagaattca cgcgtcgagc atgcatctag ggcggccaat tccgcccctc
     1021 tccccccccc ccccccctct ccctcccccc cccctaacgt tactggccga agccgcttgg
     1081 aataaggccg gtgtgcgttt gtctatatgt tattttccac catattgccg tcttttggca
     1141 atgtgagggc ccggaaacct ggccctgtct tcttgacgag cattcctagg ggtctttccc
     1201 ctctcgccaa aggaatgcaa ggtctgttga atgtcgtgaa ggaagcagtt cctctggaag
     1261 cttcttgaag acaaacaacg tctgtagcga ccctttgcag gcagcggaac cccccacctg
     1321 gcgacaggtg cctctgcggc caaaagccac gtgtataaga tacacctgca aaggcggcac
     1381 aaccccagtg ccacgttgtg agttggatag ttgtggaaag agtcaaatgg ctctcctcaa
     1441 gcgtattcaa caaggggctg aaggatgccc agaaggtacc ccattgtatg ggatctgatc
     1501 tggggcctcg gtgcacatgc tttacatgtg tttagtcgag gttaaaaaaa cgtctaggcc
     1561 ccccgaacca cggggacgtg gttttccttt gaaaaacacg atgataagct tgccacaacc
     1621 cgggatcctc tagaatggat aaaaatgagc tggttcagaa ggccaaactg gccgagcagg
     1681 ctgagcgata tgatgacatg gcagcctgca tgaagtctgt aactgagcaa ggagctgaat
     1741 tatccaatga ggagaggaat cttctctcag ttgcttataa aaatgttgta ggagcccgta
     1801 ggtcatcttg gagggtcgtc tcaagtattg aacaaaagac ggaaggtgct gagaaaaaac
     1861 agcagatggc tcgagaatac agagagaaaa ttgagacgga gctaagagat atctgcaatg
     1921 atgtactgtc tcttttggaa aagttcttga tccccaatgc ttcacaagca gagagcaaag
     1981 tcttctattt gaaaatgaaa ggagattact accgttactt ggctgaggtt gccgctggtg
     2041 atgacaagaa agggattgtc gatcagtcac aacaagcata ccaagaagct tttgaaatca
     2101 gcaaaaagga aatgcaacca acacatccta tcagactggg tctggccctt aacttctctg
     2161 tgttctatta tgagattctg aactccccag agaaagcctg ctctcttgca aagacagctt
     2221 ttgatgaagc cattgctgaa cttgatacat taagtgaaga gtcatacaaa gacagcacgc
     2281 taataatgca attactgaga gacaacttga cattgtggac atcggatacc caaggagacg
     2341 aagctgaagc aggagaagga ggggaaaatg aacaaaaact catctcagaa gaggatctgt
     2401 aatctagagt cgacccgggc ggccgcttcc ctttagtgag ggttaatgct tcgagcagac
     2461 atgataagat acattgatga gtttggacaa accacaacta gaatgcagtg aaaaaaatgc
     2521 tttatttgtg aaatttgtga tgctattgct ttatttgtaa ccattataag ctgcaataaa
     2581 caagttaaca acaacaattg cattcatttt atgtttcagg ttcaggggga gatgtgggag
     2641 gttttttaaa gcaagtaaaa cctctacaaa tgtggtaaaa tccgataagg atcgatccgg
     2701 gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc ccaacagttg cgcagcctga
     2761 atggcgaatg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg
     2821 cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc
     2881 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg
     2941 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc
     3001 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt
     3061 ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc
     3121 ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta
     3181 acaaaaattt aacgcgaatt ttaacaaaat attaacgctt acaatttcct gatgcggtat
     3241 tttctcctta cgcatctgtg cggtatttca caccgcatac gcggatctgc gcagcaccat
     3301 ggcctgaaat aacctctgaa agaggaactt ggttaggtac cttctgaggc ggaaagaacc
     3361 agctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa
     3421 gtatgcaaag catgcatctc aattagtcag caaccaggtg tggaaagtcc ccaggctccc
     3481 cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccata gtcccgcccc
     3541 taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct
     3601 gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga
     3661 agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagcttgat tcttctgaca
     3721 caacagtctc gaacttaagg ctagagccac catgattgaa caagatggat tgcacgcagg
     3781 ttctccggcc gcttgggtgg agaggctatt cggctatgac tgggcacaac agacaatcgg
     3841 ctgctctgat gccgccgtgt tccggctgtc agcgcagggg cgcccggttc tttttgtcaa
     3901 gaccgacctg tccggtgccc tgaatgaact gcaggacgag gcagcgcggc tatcgtggct
     3961 ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga
     4021 ctggctgcta ttgggcgaag tgccggggca ggatctcctg tcatctcacc ttgctcctgc
     4081 cgagaaagta tccatcatgg ctgatgcaat gcggcggctg catacgcttg atccggctac
     4141 ctgcccattc gaccaccaag cgaaacatcg catcgagcga gcacgtactc ggatggaagc
     4201 cggtcttgtc gatcaggatg atctggacga agagcatcag gggctcgcgc cagccgaact
     4261 gttcgccagg ctcaaggcgc gcatgcccga cggcgaggat ctcgtcgtga cccatggcga
     4321 tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt tctggattca tcgactgtgg
     4381 ccggctgggt gtggcggacc gctatcagga catagcgttg gctacccgtg atattgctga
     4441 agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga
     4501 ttcgcagcgc atcgccttct atcgccttct tgacgagttc ttctgagcgg gactctgggg
     4561 ttcgaaatga ccgaccaagc gacgcccaac ctgccatcac gatggccgca ataaaatatc
     4621 tttattttca ttacatctgt gtgttggttt tttgtgtgaa tcgatagcga taaggatccg
     4681 cgtatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agccccgaca
     4741 cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag
     4801 acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa
     4861 acgcgcgaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat
     4921 aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg
     4981 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat
     5041 gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat
     5101 tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt
     5161 aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag
     5221 cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa
     5281 agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg
     5341 ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct
     5401 tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac
     5461 tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca
     5521 caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat
     5581 accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact
     5641 attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc
     5701 ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga
     5761 taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg
     5821 taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg
     5881 aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca
     5941 agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta
     6001 ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca
     6061 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
     6121 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
     6181 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
     6241 tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
     6301 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
     6361 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
     6421 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
     6481 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
     6541 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
     6601 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
     6661 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct
     6721 ggccttttgc tggccttttg ctcacatggc tcgacagatc ttcaatattg gccattagcc
     6781 atattattca ttggttatat agcataaatc aatattggct attggccatt gcatacgttg
     6841 tatctatatc ataatatgta catttatatt ggctcatgtc caa
//
