LOCUS       sequence-139449-        5409 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  retroviral expression of human KRAS.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5409)
  AUTHORS   Worringer KA, Rand TA, Hayashi Y, Sami S, Takahashi K, Tanabe K, 
            Narita M, Srivastava D, Yamanaka S
  TITLE     The let-7/LIN-41 pathway regulates reprogramming to human induced 
            pluripotent stem cells by controlling expression of 
            prodifferentiation genes.
  JOURNAL   Cell Stem Cell. 2014 Jan 2;14(1):40-52. doi: 
            10.1016/j.stem.2013.11.001. Epub 2013 Nov 14.
  PUBMED    24239284
REFERENCE   2  (bases 1 to 5409)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.stem.2013.11.001"; journalName: "Cell Stem Cell"; date: 
            "2014-01-2- 2"; volume: "14"; issue: "1"; pages: "40-52"
COMMENT     Sequence Label: pMXs-hs-KRAS
FEATURES             Location/Qualifiers
     source          1..5409
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..594
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     misc_feature    657..1014
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             1073..1489
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1419..1441
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1457..1473
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     misc_feature    1499..1873
                     /label=pol region
                     /note="Moloney murine leukemia virus (MMLV) pol region 
                     containing the splice acceptor site"
     primer_bind     1811..1827
                     /label=pBMN 5'
                     /note="MMLV sequence, for inserts in pBMN retroviral 
                     vector"
     protein_bind    1924..1948
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1969..2535
                     /codon_start=1
                     /product="human small GTPase proto-oncoprotein"
                     /label=K-Ras
                     /translation="MTEYKLVVVGAGGVGKSALTIQLIQNHFVDEYDPTIEDSYRKQVV
                     IDGETCLLDILDTAGQEEYSAMRDQYMRTGEGFLCVFAINNTKSFEDIHHYREQIKRVK
                     DSEDVPMVLVGNKCDLPSRTVDTKQAQDLARSYGIPFIETSAKTRQGVDDAFYTLVREI
                     RKHKEKMSKDGKKKKKKSKTKCVIM"
     protein_bind    complement(2552..2576)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     LTR             2893..3362
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     primer_bind     complement(3492..3509)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3663..4251)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3743..3762)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4415..5206,5207..5275))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5038..5057
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5276..5380)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 aatgaaagac cccacctgta ggtttggcaa gctagcttaa gtaacgccat tttgcaaggc
       61 atggaaaaat acataactga gaatagaaaa gttcagatca aggtcaggaa cagatggaac
      121 agctgaatat gggccaaaca ggatatctgt ggtaagcagt tcctgccccg gctcagggcc
      181 aagaacagat ggaacagctg aatatgggcc aaacaggata tctgtggtaa gcagttcctg
      241 ccccggctca gggccaagaa cagatggtcc ccagatgcgg tccagccctc agcagtttct
      301 agagaaccat cagatgtttc cagggtgccc caaggacctg aaatgaccct gtgccttatt
      361 tgaactaacc aatcagttcg cttctcgctt ctgttcgcgc gcttctgctc cccgagctca
      421 ataaaagagc ccacaacccc tcactcggcg cgccagtcct ccgattgact gagtcgcccg
      481 ggtacccgtg tatccaataa accctcttgc agttgcatcc gacttgtggt ctcgctgttc
      541 cttgggaggg tctcctctga gtgattgact acccgtcagc gggggtcttt catttggggg
      601 ctcgtccggg atcgggagac ccctgcccag ggaccaccga cccaccaccg ggaggtaagc
      661 tggccagcaa cttatctgtg tctgtccgat tgtctagtgt ctatgactga ttttatgcgc
      721 ctgcgtcggt actagttagc taactagctc tgtatctggc ggacccgtgg tggaactgac
      781 gagttcggaa cacccggccg caaccctggg agacgtccca gggacttcgg gggccgtttt
      841 tgtggcccga cctgagtcca aaaatcccga tcgttttgga ctctttggtg caccccccta
      901 ataggaggga tatgtggttc tggtaggaga cgagaaccta aaacagttcc cgcctccgtc
      961 tgaatttttg ctttcggttt gggaccgaag ccgcgccgcg cgtcttgtct gctgcagcat
     1021 cgttctgtgt tgtctctgtc tgactgtgtt tctgtatttg tctgaaaatt agggccagac
     1081 tgttaccact cccttaagtt tgaccttagg tcactggaaa gatgtcgagc ggatcgctca
     1141 caaccagtcg gtagatgtca agaagagacg ttgggttacc ttctgctctg cagaatggcc
     1201 aacctttaac gtcggatggc cgcgagacgg cacctttaac cgagacctca tcacccaggt
     1261 taagatcaag gtcttttcac ctggcccgca tggacaccca gaccaggtcc cctacatcgt
     1321 gacctgggaa gccttggctt ttgacccccc tccctgggtc aagccctttg tacaccctaa
     1381 gcctccgcct cctcttcctc catccgcccc gtctctcccc cttgaacctc ctcgttcgac
     1441 cccgcctcga tcctcccttt atccagccct cactccttct ctaggcgccc ccatatggcc
     1501 atatgagatc ttatatgggg cacccccgcc ccttgtaaac ttccctgacc ctgacatgac
     1561 aagagttact aacagcccct ctctccaagc tcacttacag gctctctact tagtccagca
     1621 cgaagtctgg agacctctgg cggcagccta ccaagaacaa ctggaccgac cggtggtacc
     1681 tcacccttac cgagtcggcg acacagtgtg ggtccgccga caccagacta agaacctaga
     1741 acctcgctgg aaaggacctt acacagtcct gctgaccacc cccaccgccc tcaaagtaga
     1801 cggcatcgca gcttggatac acgccgccca cgtgaaggct gccgaccccg ggggtggacc
     1861 atcctctaga ctgccggatc tagctagtta attaaggatc ccagtgtggt ggtacgggaa
     1921 atcacaagtt tgtacaaaaa agcaggctcc gcggccgccc ccttcaccat gactgaatat
     1981 aaacttgtgg tagttggagc tggtggcgta ggcaagagtg ccttgacgat acagctaatt
     2041 cagaatcatt ttgtggacga atatgatcca acaatagagg attcctacag gaagcaagta
     2101 gtaattgatg gagaaacctg tctcttggat attctcgaca cagcaggtca agaggagtac
     2161 agtgcaatga gggaccagta catgaggact ggggagggct ttctttgtgt atttgccata
     2221 aataatacta aatcatttga agatattcac cattatagag aacaaattaa aagagttaag
     2281 gactctgaag atgtacctat ggtcctagta ggaaataaat gtgatttgcc ttctagaaca
     2341 gtagacacaa aacaggctca ggacttagca agaagttatg gaattccttt tattgaaaca
     2401 tcagcaaaga caagacaggg tgttgatgat gccttctata cattagttcg agaaattcga
     2461 aaacataaag aaaagatgag caaagatggt aaaaagaaga aaaagaagtc aaagacaaag
     2521 tgtgtaatta tgtaaaaggg tgggcgcgcc gacccagctt tcttgtacaa agtggtgata
     2581 ttccagctga gcgccggtcg ctaccattac cagttggtct ggtgtcaaaa ataataataa
     2641 ccgggcaggc catgtctgcc cgtatttcgc gtaaggaaat ccattatgta ctatttaaac
     2701 tcgagcggcc gccagcacag tggtcgacga taaaataaaa gattttattt agtctccaga
     2761 aaaagggggg aatgaaagac cccacctgta ggtttggcaa gctagcttaa gtaacgccat
     2821 tttgcaaggc atggaaaaat acataactga gaatagagaa gttcagatca aggtcaggaa
     2881 cagatggaac agctgaatat gggccaaaca ggatatctgt ggtaagcagt tcctgccccg
     2941 gctcagggcc aagaacagat ggaacagctg aatatgggcc aaacaggata tctgtggtaa
     3001 gcagttcctg ccccggctca gggccaagaa cagatggtcc ccagatgcgg tccagccctc
     3061 agcagtttct agagaaccat cagatgtttc cagggtgccc caaggacctg aaatgaccct
     3121 gtgccttatt tgaactaacc aatcagttcg cttctcgctt ctgttcgcgc gcttctgctc
     3181 cccgagctca ataaaagagc ccacaacccc tcactcgggg cgccagtcct ccgattgact
     3241 gagtcgcccg ggtacccgtg tatccaataa accctcttgc agttgcatcc gacttgtggt
     3301 ctcgctgttc cttgggaggg tctcctctga gtgattgact acccgtcagc gggggtcttt
     3361 cacatgcagc atgtatcaaa attaatttgg ttttttttct taagtattta cattaaatgg
     3421 ccatagttgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc
     3481 tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta
     3541 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag
     3601 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
     3661 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
     3721 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
     3781 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
     3841 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
     3901 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
     3961 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
     4021 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
     4081 cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt
     4141 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
     4201 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
     4261 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
     4321 gtcatgagat tatcaaaaag gatcttcacc tagatccttt tgcggccggc cgcaaatcaa
     4381 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac
     4441 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga
     4501 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc
     4561 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca
     4621 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta
     4681 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg
     4741 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc
     4801 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg
     4861 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt
     4921 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt
     4981 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata
     5041 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc
     5101 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac
     5161 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa
     5221 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct
     5281 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat
     5341 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc
     5401 cacctgcat
//
