LOCUS       sequence-139452-       10651 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Tol2 vector with fli1ep element upstream of EGFP, attR1/R2 cassette,
            and late SV40 pA signal.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10651)
  AUTHORS   Villefranc JA, Amigo J, Lawson ND
  TITLE     Gateway compatible vectors for analysis of gene function in the 
            zebrafish.
  JOURNAL   Dev Dyn. 2007 Nov . 236(11):3077-87.
  PUBMED    17948311
REFERENCE   2  (bases 1 to 10651)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Dev Dyn. 
            2007 Nov . 236(11):3077-87."
COMMENT     Sequence Label: 372 pTol2-fli1ep:EGFPDest
FEATURES             Location/Qualifiers
     source          1..10651
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(34..462)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(144..165)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     356..375
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     589..611
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     603..620
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     604..620
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        630..648
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    join(778..789,790..952)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
     regulatory      3418..3427
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(3424..3426,3427..3429,3430..4140)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(3424..3426,3427..3429,3430..4140)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3469..3490)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3730..3749)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4077..4098
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    4162..4286
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(4311..4316,4317..4334,4335..4341)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             4395..5054
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(4461..4480)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             5396..5701
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     5580..5599
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(5742..5866)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     polyA_signal    5906..6040
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5930..5949)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     5984..6003
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     misc_feature    join(8181..8368,8369..8380)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     primer_bind     complement(8414..8430)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8414..8430)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(8467..8487)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(8467..8485)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(8506..8522)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8506..8522)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8519..8541)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8530..8546
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8554..8560,8561..8578,8579..8584))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8599..8620
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8737..8754)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8908..9496)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8988..9007)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9667..10458,10459..10527))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10290..10309
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10528..10632)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ccacctaaat tgtaagcgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca
       61 gctcattttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga
      121 ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg
      181 actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacta cgtgaaccat
      241 caccctaatc aagttttttg gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag
      301 ggagcccccg atttagagct tgacggggaa agccggcgaa cgtggcgaga aaggaaggga
      361 agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa
      421 ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc gtcccattcg ccattcaggc
      481 tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc gctattacgc cagctggcga
      541 aagggggatg tgctgcaagg cgattaagtt gggtaacgcc agggttttcc cagtcacgac
      601 gttgtaaaac gacggccagt gagcgcgcgt aatacgactc actatagggc gaattgggta
      661 caggtcattg cacaacacca gaaatgccct ctgatctgca aaagacgtga atatctgttc
      721 agacacccat atccactctg ttccacacag gtcagaggtt tgtccaggag ttcttgacag
      781 aggtgtaaaa agtactcaaa aattttactc aagtgaaagt acaagtactt agggaaaatt
      841 ttactcaatt aaaagtaaaa gtatctggct agaatcttac ttgagtaaaa gtaaaaaagt
      901 actccattaa aattgtactt gagtattaag gaagtaaaag taaaagcaag aaagaaaact
      961 agagattctt gtttaagctt ttaatctcaa aaaacattaa atgaaatgca tacaaggttt
     1021 tatcctgctt tagaactgtt tgtatttaat tatcaaacta taagacagac aatctaatgc
     1081 cagtacacgc tactcaaagt tgtaaaacct cagatttaac ttcagtagaa gctgattctc
     1141 aaaattgtta gtgtcaagcc tagctctttt ggggctgaaa agcaatcctg cagtgctgaa
     1201 aagcctctca caggcagccg atgcgggaag aggtgtatta gtcttgatag agaggctgca
     1261 aatagcagga aacgtgagca gagactccct ggtgtctgaa acacaggcca gatgggccga
     1321 taattcgccc ttggagatct catctttgac ccataaacat acactaaaac ccaacagtca
     1381 actttatcaa atgaaatgag tgtaattaac tcaaaattta ctgaatgttg attctactca
     1441 ttcgaaaaga gttttaaact cagtgttgaa ggtagtgagt tcattaaata cctcattact
     1501 tcaacttaac tggagtaagt tcatggtact cacatagatt agtttagctc aaatggtttg
     1561 tagcagtcgg ttccctcaag cagtttgagt tgccttaact ttttgggttt tacagtactc
     1621 agttggtttg agttcacttc acttattggg ttcaacatgt gctcaaattg catcgtttaa
     1681 tcaaatagat taagttcaca gtactcatta ggattagttt ttgaacttaa atggtttgtt
     1741 gcagtcggtt tcctcaaacg gtttgagttt ccataacttt atgggttttt cagtgtagta
     1801 aacatatgca tccacagatt ataatagtaa cctgcatgtt ttttccttca caatttgaca
     1861 cagatacctc acatgattga cttttaactg tacactttag tcgacaatga gaagggtgtc
     1921 tcaatctatg ggtatttcct gttccacaca atctggtttc ctgtcaggtt tccttttcct
     1981 cctgaaacaa aagacaggaa tgccctctgt ataaaatagc acagcgcttt taatgggtct
     2041 ctgtacatcc cgacctcatg acgcatcttc tgcgggacag ctcaatcacg atcaagttgg
     2101 cttatgaaaa cagttttttg tccccctgtt aagttaaaag cacggtagca tacatgcatt
     2161 attcatcatg cagcatattc agcagtacaa aagatgagct cttgacacac ctcagcgtaa
     2221 aatctctatt ccagcgtaat cctgcggttc tggatggtta agcgagcctt gcaaacacgg
     2281 ccaatttata gccatgttga aaatagcagc tttgtgagca tttcgacctc aatgtaccaa
     2341 gaatgcgtgc tcggtaactg aaaaagaggg ggtggagatt ttggatttag agatggaatc
     2401 acatgcacgc tgtatatgct atataaaaat agcaggcatt taatgcatgc aaaatatgtt
     2461 tcgacactaa acactacaag tcggaaatat atctattttt ctttttttaa cgaaatgaga
     2521 catttaatgc gcagtaaatg tagtctaaca cgcacaatat tcctattatt gtccatgaaa
     2581 aatgtgaggt crtatgctac tgatttaaag aaaatgttga acaggcacag cacacaagag
     2641 gtatcagcct ctctatatgg tccatacagc accaggctcg ctgcgttcat gtgcattcag
     2701 aggcgcgcac acgtgtggag cagacagact cgacgcagcg ggctttgctt tttattcatg
     2761 gaagcccatt ttagaagaag ctcgggcaga ctcgtcacat tcttcacttc accataggta
     2821 gatcaaatca aagttattgg atataacgga acaatattca tcatattatg ragattatta
     2881 ttaagaccga gtgcattaga catattttgt atttcgatcg ttggaaaaag tatatatttt
     2941 attttagcct taaactatgg ttagcctata ttaatcagca taatctgtta tttcagacgt
     3001 tgtattaatg attatttcta gcacagcttt gatgcatcaa tgcaatgttt ctacaaaccc
     3061 gccttgttta atgtcggaaa aaaagcgcaa caaatgtaaa gagttggcag gcattctgta
     3121 aatatttcca ggcaaaaaca tgcacaaagg aaagaaacac cattgttttg gtgtcttatc
     3181 ttcgctggac aaatatcctg ctttgggtgc cgtaatgaag gcagggccag tgacacacac
     3241 acatacacac acacacatac acgcatacac ggcttccttc tcaagcgcag tgacttcact
     3301 tcccaaattt agaggaaaaa aaaccatccg gcgaaactgt ctctgtctct ccgccacata
     3361 tcgggggctg gaattaattc agacgcgaag ggcgaattat cgctagcgct accggtcgcc
     3421 accatggtga gcaagggcga ggagctgttc accggggtgg tgcccatcct ggtcgagctg
     3481 gacggcgacg taaacggcca caagttcagc gtgtccggcg agggcgaggg cgatgccacc
     3541 tacggcaagc tgaccctgaa gttcatctgc accaccggca agctgcccgt gccctggccc
     3601 accctcgtga ccaccctgac ctacggcgtg cagtgcttca gccgctaccc cgaccacatg
     3661 aagcagcacg acttcttcaa gtccgccatg cccgaaggct acgtccagga gcgcaccatc
     3721 ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg tgaagttcga gggcgacacc
     3781 ctggtgaacc gcatcgagct gaagggcatc gacttcaagg aggacggcaa catcctgggg
     3841 cacaagctgg agtacaacta caacagccac aacgtctata tcatggccga caagcagaag
     3901 aacggcatca aggtgaactt caagatccgc cacaacatcg aggacggcag cgtgcagctc
     3961 gccgaccact accagcagaa cacccccatc ggcgacggcc ccgtgctgct gcccgacaac
     4021 cactacctga gcacccagtc cgccctgagc aaagacccca acgagaagcg cgatcacatg
     4081 gtcctgctgg agttcgtgac cgccgccggg atcactctcg gcatggacga gctgtacaag
     4141 tccggactca gatctcgaat cacaagtttg tacaaaaaag ctgaacgaga aacgtaaaat
     4201 gatataaata tcaatatatt aaattagatt ttgcataaaa aacagactac ataatactgt
     4261 aaaacacaac atatccagtc actatggcgg ccgcattagg caccccaggc tttacacttt
     4321 atgcttccgg ctcgtataat gtgtggattt tgagttagga tccgtcgaga ttttcaggag
     4381 ctaaggaagc taaaatggag aaaaaaatca ctggatatac caccgttgat atatcccaat
     4441 ggcatcgtaa agaacatttt gaggcatttc agtcagttgc tcaatgtacc tataaccaga
     4501 ccgttcagct ggatattacg gcctttttaa agaccgtaaa gaaaaataag cacaagtttt
     4561 atccggcctt tattcacatt cttgcccgcc tgatgaatgc tcatccggaa ttccgtatgg
     4621 caatgaaaga cggtgagctg gtgatatggg atagtgttca cccttgttac accgttttcc
     4681 atgagcaaac tgaaacgttt tcatcgctct ggagtgaata ccacgacgat ttccggcagt
     4741 ttctacacat atattcgcaa gatgtggcgt gttacggtga aaacctggcc tatttcccta
     4801 aagggtttat tgagaatatg tttttcgtct cagccaatcc ctgggtgagt ttcaccagtt
     4861 ttgatttaaa cgtggccaat atggacaact tcttcgcccc cgttttcacc atgggcaaat
     4921 attatacgca aggcgacaag gtgctgatgc cgctggcgat tcaggttcat catgccgttt
     4981 gtgatggctt ccatgtcggc agaatgctta atgaattaca acagtactgc gatgagtggc
     5041 agggcggggc gtaaacgcgt ggatccggct tactaaaagc cagataacag tatgcgtatt
     5101 tgcgcgctga tttttgcggt ataagaatat atactgatat gtatacccga agtatgtcaa
     5161 aaagaggtat gctatgaagc agcgtattac agtgacagtt gacagcgaca gctatcagtt
     5221 gctcaaggca tatatgatgt caatatctcc ggtctggtaa gcacaaccat gcagaatgaa
     5281 gcccgtcgtc tgcgtgccga acgctggaaa gcggaaaatc aggaagggat ggctgaggtc
     5341 gcccggttta ttgaaatgaa cggctctttt gctgacgaga acaggggctg gtgaaatgca
     5401 gtttaaggtt tacacctata aaagagagag ccgttatcgt ctgtttgtgg atgtacagag
     5461 tgatattatt gacacgcccg ggcgacggat ggtgatcccc ctggccagtg cacgtctgct
     5521 gtcagataaa gtctcccgtg aactttaccc ggtggtgcat atcggggatg aaagctggcg
     5581 catgatgacc accgatatgg ccagtgtgcc ggtctccgtt atcggggaag aagtggctga
     5641 tctcagccac cgcgaaaatg acatcaaaaa cgccattaac ctgatgttct ggggaatata
     5701 aatgtcaggc tcccttatac acagccagtc tgcaggtcga ccatagtgac tggatatgtt
     5761 gtgttttaca gcattatgta gtctgttttt tatgcaaaat ctaatttaat atattgatat
     5821 ttatatcatt ttacgtttct cgttcagctt tcttgtacaa agtggtgatt cgagcctcta
     5881 gaactatagt gagtcgtatt acgtagatcc agacatgata agatacattg atgagtttgg
     5941 acaaaccaca actagaatgc agtgaaaaaa atgctttatt tgtgaaattt gtgatgctat
     6001 tgctttattt gtaaccatta taagctgcaa taaacaagtt aacaacaaca attgcattca
     6061 ttttatgttt caggttcagg gggaggtgtg ggaggttttt taattcgcgg ccgcggcgcc
     6121 aatgcattgg gccctcgagc atatgagatc tgtcccgatc tttctcttct gtgctgtcaa
     6181 tttagagtag tttttgaggt aatttgggtg cattctctgt aaaaacaaca aataaatatt
     6241 tatattgtat gcaataaatg taacaattac acatacactc tctgattaca gcattaaagg
     6301 gttagttcac ccaaaaatga aaagatgtca ttaatgactc accctcatgt cgttccaagc
     6361 ccgtaacacc tccgttcatc ttcggaacac agtttaagat attttagatt tagtccgaga
     6421 gctttctgtc cctccactga aaatgtatgt acggtatact gtccatgtcc agaaaggtaa
     6481 taaaaacatc aaagtagtcc atgtgacatc agtgggttag gtagaattat ttgaagcatc
     6541 gaaaatacat tttggtccaa aaataacaaa acctacgact ttattcagca ttgtagcgtc
     6601 actgcgaagt cgtgaacgcg gattgacaac agacccggaa gagaatacaa tgccgaataa
     6661 agtcgtaggt tttgttattt ttggaccaaa atgtattcga tgcttcaaaa aattctaact
     6721 aacccactga tgtcacatgg actactttga tgtttttatt acctttctgg acatggacag
     6781 tataccgtac atacattctc aatggaggca cagaaagctc tcggactaaa tctaaaatat
     6841 cttaaactgt gttctgaaga tgaacggagg tcttacgggc ttggaacgac atgagggcga
     6901 gtcattaatg acattatttt catttttggg tgaactaacc ctttaataaa actgacattt
     6961 gcagccaatt gcacacttta taatgttaaa tacttgttga ttttagaaat acagtagcat
     7021 ttagtgacaa tcaaacatta attttatttc tgattatatt gacaaaaaca caggactgta
     7081 aggctgacat gacagggtga attgatttca ttacattagt tttgatggta acacttttac
     7141 aataaggttc gttagttaac tacattagtt aacatgaact aataatgaac tgcacgtata
     7201 catgttaatt tcaacattta ctaatacttt atatcttgtt aacattagtt aatgcactgt
     7261 gaactaacat gaacaaacaa tgaacatctg tatttctatt aactaacgtt aacaaagatt
     7321 aacagataca gtaacaaatg tattgctcat ggttatttaa tgttagttaa tacatgaact
     7381 aatcaacctc attgtaaagt gttaccgttt tgacaataca acatgtttgg aaaaaaattt
     7441 ctcgcttggt tttcttgtgt tttgcatgaa ttcaaacaca atgagagcac ggaatgataa
     7501 tgattattca aagctgcaat aggcgccaaa tttcccgtgg acctaacgtt accaattaca
     7561 aatgcatttt agcattttat tacgtgagcc ttatattgtc aatcgcatgc attagtgaaa
     7621 ataggcctac tacacatata aaaggcgcgc catcaacgtc aaaacatgca cacccaaaaa
     7681 aaaaaaaaaa aaaaaaaaaa aaaactatca gtaaaacaaa atacttaatg tacttacctc
     7741 aatatgcttc cttaggtttg atggcgaact tttgaaggcc gatatttctt tattaagcgg
     7801 gagacagagg acacatttca tcttgaatga atctttattt acaccactta aagaaaagaa
     7861 ttcgcgaaga tacggccacg ggtgctcttg atcctgtggc tgattttgga ctgtgctgct
     7921 cgcagctgct gatgaatcac atacttcctc cattttcttc cactgattga ctgttataat
     7981 ttccctaatt tccaggtcaa ggtgctgtgc attgtggtaa tagatgtgac atgacgtcac
     8041 ttccaaagga ccaatgaaca tgtctgacca atttcatata atgtgaaaac gattttcata
     8101 ggcagaataa ataacattta aattaaactg ggcatcagcg caattcaatt ggtttggtaa
     8161 tagcaaggga aaatagaatg aagtgatctc caaaaaataa gtactttttg actgtaaata
     8221 aaattgtaag gagtaaaaag tacttttttt tctaaaaaaa tgtaattaag taaaagtaaa
     8281 agtattgatt tttaattgta ctcaagtaaa gtaaaaatcc ccaaaaataa tacttaagta
     8341 cagtaatcaa gtaaaattac tcaagtactt tacacctctg gttcttgacc ccctaccttc
     8401 agcaagccca gcagatccac tagttctaga gcggccgcca ccgcggtgga gctccagctt
     8461 ttgttccctt tagtgagggt taattgcgcg cttggcgtaa tcatggtcat agctgtttcc
     8521 tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg
     8581 taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc
     8641 cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg
     8701 gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc
     8761 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac
     8821 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa
     8881 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca
     8941 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
     9001 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
     9061 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta
     9121 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
     9181 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
     9241 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
     9301 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg
     9361 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
     9421 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
     9481 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
     9541 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat
     9601 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc
     9661 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc
     9721 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc
     9781 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc
     9841 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc
     9901 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt
     9961 gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc
    10021 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa
    10081 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt
    10141 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg
    10201 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc
    10261 gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa
    10321 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt
    10381 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt
    10441 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag
    10501 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta
    10561 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat
    10621 aggggttccg cgcacatttc cccgaaaagt g
//
