LOCUS       sequence-139456-        7271 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses VP1 and VP3 for Adeno-associated virus serotype 2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7271)
  AUTHORS   Gomez EJ, Gerhardt K, Judd J, Tabor JJ, Suh J
  TITLE     Light-Activated Nuclear Translocation of Adeno-Associated Virus 
            Nanoparticles Using Phytochrome B for Enhanced, Tunable, and 
            Spatially Programmable Gene Delivery.
  JOURNAL   ACS Nano. 2015 Nov 30.
  PUBMED    26618393
REFERENCE   2  (bases 1 to 7271)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Nano. 
            2015 Nov 30."
COMMENT     Sequence Label: pVP1/3
FEATURES             Location/Qualifiers
     source          1..7271
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(30..458)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(140..161)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     352..371
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     585..607
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     599..616
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     626..645
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        626..644
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     669..685
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     670..686
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(720..736)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(720..736)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     CDS             4799..4810
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     primer_bind     complement(5083..5103)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(5083..5101)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(5122..5138)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5122..5138)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5135..5157)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5146..5162
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5170..5176,5177..5194,5195..5200))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5215..5236
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5353..5370)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5524..6112)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5604..5623)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6283..7074,7075..7143))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6906..6925
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7144..7248)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
      301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
      421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgagc gcgcgtaata cgactcacta tagggcgaat tgggtaccgg
      661 gccccccctc gaggtcgacg gtatcgataa gcttgatatc gaattcctgc agcccggggg
      721 atccactagt tctagaggtc ctgtattaga ggtcacgtga gtgttttgcg acattttgcg
      781 acaccatgtg gtcacgctgg gtatttaagc ccgagtgagc acgcagggtc tccattttga
      841 agcgggaggt ttgaacgcgc agccgccatg ccggggtttt acgagattgt gattaaggtc
      901 cccagcgacc ttgacgagca tctgcccggc atttctgaca gctttgtgaa ctgggtggcc
      961 gagaaggaat gggagttgcc gccagattct gacatggatc tgaatctgat tgagcaggca
     1021 cccctgaccg tggccgagaa gctgcagcgc gactttctga cggaatggcg ccgtgtgagt
     1081 aaggccccgg aggccctttt ctttgtgcaa tttgagaagg gagagagcta cttccacatg
     1141 cacgtgctcg tggaaaccac cggggtgaaa tccatggttt tgggacgttt cctgagtcag
     1201 attcgcgaaa aactgattca gagaatttac cgcgggatcg agccgacttt gccaaactgg
     1261 ttcgcggtca caaagaccag aaatggcgcc ggaggcggga acaaggtggt ggatgagtgc
     1321 tacatcccca attacttgct ccccaaaacc cagcctgagc tccagtgggc gtggactaat
     1381 atggaacagt atttaagcgc ctgtttgaat ctcacggagc gtaaacggtt ggtggcgcag
     1441 catctgacgc acgtgtcgca gacgcaggag cagaacaaag agaatcagaa tcccaattct
     1501 gatgcgccgg tgatcagatc aaaaacttca gccaggtaca tggagctggt cgggtggctc
     1561 gtggacaagg ggattacctc ggagaagcag tggatacagg aggaccaggc ctcatacatc
     1621 tccttcaatg cggcctccaa ctcgcggtcc caaatcaagg ctgccttgga caatgcggga
     1681 aagattatga gcctgactaa aaccgccccc gactacctgg tgggccagca gcccgtggag
     1741 gacatttcca gcaatcggat ttataaaatt ttggaactaa acgggtacga tccccaatat
     1801 gcggcttccg tctttctggg atgggccacg aaaaagttcg gcaagaggaa caccatctgg
     1861 ctgtttgggc ctgcaactac cgggaagacc aacatcgcgg aggccatagc ccacactgtg
     1921 cccttctacg ggtgcgtaaa ctggaccaat gagaactttc ccttcaacga ctgtgtcgac
     1981 aagatggtga tctggtggga ggaggggaag atgaccgcca aggtcgtgga gtcggccaaa
     2041 gccattctcg gaggaagcaa ggtgcgcgtg gaccagaaat gcaagtcctc ggcccagata
     2101 gacccgactc ccgtgatcgt cacctccaac accaacatgt gcgccgtgat tgacgggaac
     2161 tcaacgacct tcgaacacca gcagccgttg caagaccgga tgttcaaatt tgaactcacc
     2221 cgccgtctgg atcatgactt tgggaaggtc accaagcagg aagtcaaaga ctttttccgg
     2281 tgggcaaagg atcacgtggt tgaggtggag catgaattct acgtcaaaaa gggtggagcc
     2341 aagaaaagac ccgcccccag tgacgcagat ataagtgagc ccaaacgggt gcgcgagtca
     2401 gttgcgcagc catcgacgtc agacgcggaa gcttcgatca actacgcaga caggtaccaa
     2461 aacaaatgtt ctcgtcacgt gggcatgaat ctgatgctgt ttccctgcag acaatgcgag
     2521 agaatgaatc agaattcaaa tatctgcttc actcacggac agaaagactg tttagagtgc
     2581 tttcccgtgt cagaatctca acccgtttct gtcgtcaaaa aggcgtatca gaaactgtgc
     2641 tacattcatc atatcatggg aaaggtgcca gacgcttgca ctgcctgcga tctggtcaat
     2701 gtggatttgg atgactgcat ctttgaacaa taaatgattt aaatcaggta tggctgccga
     2761 tggttatctt ccagattggc tcgaggacac tctctctgaa ggaataagac agtggtggaa
     2821 gctcaaacct ggcccaccac caccaaagcc cgcagagcgg cataaggacg acagcagggg
     2881 tcttgtgctt cctgggtaca agtacctcgg acccttcaac ggactcgaca agggagagcc
     2941 ggtcaacgag gcagacgccg cggccctcga gcacgacaaa gcctacgacc ggcagctcga
     3001 cagcggagac aacccgtacc tcaagtacaa ccacgccgac gcggagtttc aggagcgcct
     3061 taaagaagat acgtcttttg ggggcaacct cggacgagca gtcttccagg cgaaaaagag
     3121 ggttcttgaa cctctgggcc tggttgagga acctgttaag aaggctccgg gaaaaaagag
     3181 gccggtagag cactctcctg tggagccaga ctcctcctcg ggaaccggaa aggcgggcca
     3241 gcagcctgca agaaaaagat tgaattttgg tcagactgga gacgcagact cagtacctga
     3301 cccccagcct ctcggacagc caccagcagc cccctctggt ctgggaacta ataccatggc
     3361 tacaggcagt ggcgcaccaa tggcagacaa taacgagggt gccgacggag tgggtaattc
     3421 ctcgggaaat tggcattgcg attccacatg gatgggcgac agagtcatca ccaccagcac
     3481 ccgaacctgg gccctgccca cctacaacaa ccacctctac aaacaaattt ccagccaatc
     3541 aggagcctcg aacgacaatc actactttgg ctacagcacc ccttgggggt attttgactt
     3601 caacagattc cactgccact tttcaccacg tgactggcaa agactcatca acaacaactg
     3661 gggattccga cccaagagac tcaacttcaa gctctttaac attcaagtca aagaggtcac
     3721 gcagaatgac ggtacgacga cgattgccaa taaccttacc agcacggttc aggtgtttac
     3781 tgactcggag taccagctcc cgtacgtcct cggctcggcg catcaaggat gcctcccgcc
     3841 gttcccagca gacgtcttca tggtgccaca gtatggatac ctcaccctga acaacgggag
     3901 tcaggcagta ggacgctctt cattttactg cctggagtac tttccttctc agatgctgcg
     3961 taccggaaac aactttacct tcagctacac ttttgaggac gttcctttcc acagcagcta
     4021 cgctcacagc cagagtctgg accgtctcat gaatcctctc atcgaccagt acctgtatta
     4081 cttgagcaga acaaacactc caagtggaac caccacgcag tcaaggcttc agttttctca
     4141 ggccggagcg agtgacattc gggaccagtc taggaactgg cttcctggac cctgttaccg
     4201 ccagcagcga gtatcaaaga catctgcgga taacaacaac agtgaatact cgtggactgg
     4261 agctaccaag taccacctca atggcagaga ctctctggtg aatccgggcc cggccatggc
     4321 aagccacaag gacgatgaag aaaagttttt tcctcagagc ggggttctca tctttgggaa
     4381 gcaaggctca gagaaaacaa atgtggacat tgaaaaggtc atgattacag acgaagagga
     4441 aatcaggaca accaatcccg tggctacgga gcagtatggt tctgtatcta ccaacctcca
     4501 gagaggcaac agacaagcag ctaccgcaga tgtcaacaca caaggcgttc ttccaggcat
     4561 ggtctggcag gacagagatg tgtaccttca ggggcccatc tgggcaaaga ttccacacac
     4621 ggacggacat tttcacccct ctcccctcat gggtggattc ggacttaaac accctcctcc
     4681 acagattctc atcaagaaca ccccggtacc tgcgaatcct tcgaccacct tcagtgcggc
     4741 aaagtttgct tccttcatca cacagtactc cacgggacag gtcagcgtgg agatcgagtg
     4801 ggagctgcag aaggaaaaca gcaaacgctg gaatcccgaa attcagtaca cttccaacta
     4861 caacaagtct gttaatgtgg actttactgt ggacactaat ggcgtgtatt cagagcctcg
     4921 ccccattggc accagatacc tgactcgtaa tctgtaattg cttgttaatc aataaaccgt
     4981 ttaattcgtt tcagttgaac tttggtctct gcgtatttct ttcttatcta gtttccatgc
     5041 tctagagcgg ccgccaccgc ggtggagctc cagcttttgt tccctttagt gagggttaat
     5101 tgcgcgcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac
     5161 aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt
     5221 gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc
     5281 gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg
     5341 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
     5401 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
     5461 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
     5521 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
     5581 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
     5641 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
     5701 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
     5761 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
     5821 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
     5881 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
     5941 gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt
     6001 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
     6061 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
     6121 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
     6181 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
     6241 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag
     6301 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt
     6361 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc
     6421 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc
     6481 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg
     6541 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac
     6601 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg
     6661 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc
     6721 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact
     6781 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc
     6841 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat
     6901 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc
     6961 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac
     7021 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa
     7081 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact
     7141 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg
     7201 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg
     7261 aaaagtgcca c
//
