LOCUS       sequence-139469-         994 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses a tandem GFP-APEX2 fusion to the C-terminus of connexin43 
            in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 994)
  AUTHORS   Lam SS, Martell JD, Kamer KJ, Deerinck TJ, Ellisman MH, Mootha VK, 
            Ting AY
  TITLE     Directed evolution of APEX2 for electron microscopy and proximity 
            labeling.
  JOURNAL   Nat Methods. 2014 Nov 24. doi: 10.1038/nmeth.3179.
  PUBMED    25419960
REFERENCE   2  (bases 1 to 994)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2014 Nov 24. doi: 10.1038/nmeth.3179."
COMMENT     Sequence Label: pcDNA3 Connexin43-GFP-APEX2
FEATURES             Location/Qualifiers
     source          1..994
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(30..776)
                     /codon_start=1
                     /product="soybean ascorbate peroxidase, engineered to serve
                     as a monomeric tag for electron microscopy and live-cell 
                     proteomics (Lam et al., 2015)"
                     /label=APEX2
                     /note="more active than the original APEX due to the A134P 
                     mutation"
                     /translation="GKSYPTVSADYQDAVEKAKKKLRGFIAEKRCAPLMLRLAFHSAGT
                     FDKGTKTGGPFGTIKHPAELAHSANNGLDIAVRLLEPLKAEFPILSYADFYQLAGVVAV
                     EVTGGPKVPFHPGREDKPEPPPEGRLPDPTKGSDHLRDVFGKAMGLTDQDIVALSGGHT
                     IGAAHKERSGFEGPWTSNPLIFDNSYFTELLSGEKEGLLQLPSDKALLSDPVFRPLVDK
                     YAADEDAFFADYAEAHQKLSELGFADA"
     primer_bind     complement(858..879)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 gtttaaacgg gccctctaga ctcgagttag gcatcagcaa acccaagctc ggaaagcttt
       61 tggtgagcct cagcgtaatc agcaaagaag gcatcttcgt ccgctgcata tttgtcaacg
      121 agagggcgga atacagggtc agacaaaaga gccttgtcag aaggtagctg aaggagacct
      181 tccttctcac cactcaacaa ctccgtgaag tatgagttgt cgaaaataag aggattagag
      241 gtccagggac cctcaaatcc agaacgctcc ttgtgtgcag ctccaatagt gtgaccccca
      301 gatagagcaa cgatatcttg gtcagtaagc cccatagctt tgccaaacac atctctcaaa
      361 tggtcagaac ccttagtggg atcgggcaag cgaccctctg gtggtggctc aggcttgtcc
      421 tctcttccag ggtggaatgg aaccttaggt ccacccgtga cctcaacggc aacaacgcca
      481 gccaactggt agaaatcggc gtagctcaaa atagggaact ccgccttgag tggctccaaa
      541 agcctaacag cgatgtcaag accgttgtta gcgctgtgag ccagttcggc agggtgcttg
      601 atggttccga agggtccacc ggtcttcgtg cccttgtcaa aggttccagc agagtggaat
      661 gccaaacgga gcattagagg agcgcatctc ttctcagcga tgaagcctct gagcttcttc
      721 ttcgccttct caacggcgtc ctggtaatca gcactcacag ttgggtaaga ctttccgccc
      781 gagcccgagg tcgagcccga gcccttggcg cgccccttgt acagctcgtc catgccgaga
      841 gtgatcccgg cggcggtcac gaactccagc aggaccatgt gatcgcgctt ctcgttgggg
      901 tctttgctca gggcggactg ggtgctcagg tagtggttgt cgggcagcag cacggggccg
      961 tcgccgatgg gggtgttctg ctggtagtgg tcgg
//
