LOCUS       sequence-139473-       10169 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10169)
  AUTHORS   Fasano CA, Dimos JT, Ivanova NB, Lowry N, Lemischka IR, Temple S
  TITLE     shRNA knockdown of Bmi-1 reveals a critical role for p21-Rb pathway 
            in NSC self-renewal during development.
  JOURNAL   Cell Stem Cell. 2007 Jun 7. 1(1):87-99.
  PUBMED    18371338
REFERENCE   2  (bases 1 to 10169)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Stem 
            Cell. 2007 Jun 7. 1(1):87-99."
COMMENT     Sequence Label: Bmi1 shRNA1
FEATURES             Location/Qualifiers
     source          1..10169
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..400
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     1049..1068
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     regulatory      1175..1184
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(1181..1183,1184..1186,1187..1900)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(1181..1183,1184..1186,1187..1897)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1226..1247)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1487..1506)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1834..1855
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    1927..2515
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(1980..2000)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2398..2409)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(2518..2534)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2519..2535)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             3040..3220
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(3237..3254)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3243..3467
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      3513..3941
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3600..3619)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3810..3831
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(3950..3970)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        3955..4284
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4135..4270
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     4197..4216
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        4332..4379
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             4398..4772
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    4902..5023
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4939..4958)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4993..5012
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5072..5088)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5072..5088)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5085..5107)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5096..5112
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5120..5126,5127..5144,5145..5150))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5165..5186
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5303..5320)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5474..6062)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5554..5573)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6233..7024,7025..7093))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6856..6875
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7094..7198)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(7273..7292)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        7464..7843
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        7845..8043
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     7998..8018
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             8061..8241
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8288..8413
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8906..9139
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9324..9368
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    9666..9783
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        complement(9927..10141)
                     /label=H1 promoter
                     /note="human H1 RNA promoter"
     primer_bind     complement(9987..10006)
                     /label=H1
                     /note="Human H1 promoter, forward primer"
ORIGIN
        1 ggcctccgcg ccgggttttg gcgcctcccg cgggcgcccc cctcctcacg gcgagcgctg
       61 ccacgtcaga cgaagggcgc agcgagcgtc ctgatccttc cgcccggacg ctcaggacag
      121 cggcccgctg ctcataagac tcggccttag aaccccagta tcagcagaag gacattttag
      181 gacgggactt gggtgactct agggcactgg ttttctttcc agagagcgga acaggcgagg
      241 aaaagtagtc ccttctcggc gattctgcgg agggatctcc gtggggcggt gaacgccgat
      301 gattatataa ggacgcgccg ggtgtggcac agctagttcc gtcgcagccg ggatttgggt
      361 cgcggttctt gtttgtggat cgctgtgatc gtcacttggt gagtagcggg ctgctgggct
      421 ggccggggct ttcgtggccg ccgggccgct cggtgggacg gaagcgtgtg gagagaccgc
      481 caagggctgt agtctgggtc cgcgagcaag gttgccctga actgggggtt ggggggagcg
      541 cagcaaaatg gcggctgttc ccgagtcttg aatggaagac gcttgtgagg cgggctgtga
      601 ggtcgttgaa acaaggtggg gggcatggtg ggcggcaaga acccaaggtc ttgaggcctt
      661 cgctaatgcg ggaaagctct tattcgggtg agatgggctg gggcaccatc tggggaccct
      721 gacgtgaagt ttgtcactga ctggagaact cggtttgtcg tctgttgcgg gggcggcagt
      781 tatggcggtg ccgttgggca gtgcacccgt acctttggga gcgcgcgccc tcgtcgtgtc
      841 gtgacgtcac ccgttctgtt ggcttataat gcagggtggg gccacctgcc ggtaggtgtg
      901 cggtaggctt ttctccgtcg caggacgcag ggttcgggcc tagggtaggc tctcctgaat
      961 cgacaggcgc cggacctctg gtgaggggag ggataagtga ggcgtcagtt tctttggtcg
     1021 gttttatgta cctatcttct taagtagctg aagctccggt tttgaactat gcgctcgggg
     1081 ttggcgagtg tgttttgtga agttttttag gcaccttttg aaatgtaatc atttgggtca
     1141 atatgtaatt ttcagtgtta gactagtacc ggtcgccacc atggtgagca agggcgagga
     1201 gctgttcacc ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa acggccacaa
     1261 gttcagcgtg tccggcgagg gcgagggcga tgccacctac ggcaagctga ccctgaagtt
     1321 catctgcacc accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca ccctgaccta
     1381 cggcgtgcag tgcttcagcc gctaccccga ccacatgaag cagcacgact tcttcaagtc
     1441 cgccatgccc gaaggctacg tccaggagcg caccatcttc ttcaaggacg acggcaacta
     1501 caagacccgc gccgaggtga agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa
     1561 gggcatcgac ttcaaggagg acggcaacat cctggggcac aagctggagt acaactacaa
     1621 cagccacaac gtctatatca tggccgacaa gcagaagaac ggcatcaagg tgaacttcaa
     1681 gatccgccac aacatcgagg acggcagcgt gcagctcgcc gaccactacc agcagaacac
     1741 ccccatcggc gacggccccg tgctgctgcc cgacaaccac tacctgagca cccagtccgc
     1801 cctgagcaaa gaccccaacg agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc
     1861 cgccgggatc actctcggca tggacgagct gtacaagtaa agcaattcga tatcaagctt
     1921 atcgataatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat
     1981 gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct
     2041 tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag
     2101 gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc
     2161 cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc
     2221 ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct
     2281 cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga aatcatcgtc ctttccttgg
     2341 ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg
     2401 gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg
     2461 cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt gggccgcctc cccgcatcga
     2521 taccgtcgac ctcgagacct agaaaaacat ggagcaatca caagtagcaa tacagcagct
     2581 accaatgctg attgtgcctg gctagaagca caagaggagg aggaggtggg ttttccagtc
     2641 acacctcagg tacctttaag accaatgact tacaaggcag ctgtagatct tagccacttt
     2701 ttaaaagaaa aggggggact ggaagggcta attcactccc aacgaagaca agatatcctt
     2761 gatctgtgga tctaccacac acaaggctac ttccctgatt ggcagaacta cacaccaggg
     2821 ccagggatca gatatccact gacctttgga tggtgctaca agctagtacc agttgagcaa
     2881 gagaaggtag aagaagccaa tgaaggagag aacacccgct tgttacaccc tgtgagcctg
     2941 catgggatgg atgacccgga gagagaagta ttagagtgga ggtttgacag ccgcctagca
     3001 tttcatcaca tggcccgaga gctgcatccg gactgtactg ggtctctctg gttagaccag
     3061 atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc
     3121 ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga
     3181 tccctcagac ccttttagtc agtgtggaaa atctctagca gaaacccgct gatcagcctc
     3241 gactgtgcct tctagttgcc agccatctgt tgtttgcccc tcccccgtgc cttccttgac
     3301 cctggaaggt gccactccca ctgtcctttc ctaataaaat gaggaaattg catcgcattg
     3361 tctgagtagg tgtcattcta ttctgggggg tggggtgggg caggacagca agggggagga
     3421 ttgggaagac aatagcaggc atgctgggga tgcggtgggc tctatggctt ctgaggcgga
     3481 aagaaccagc tggggctcta gggggtatcc ccacgcgccc tgtagcggcg cattaagcgc
     3541 ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc
     3601 tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct
     3661 aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa
     3721 acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc
     3781 tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact
     3841 caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg
     3901 gttaaaaaat gagctgattt aacaaaaatt taacgcgaat taattctgtg gaatgtgtgt
     3961 cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat
     4021 ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg
     4081 caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg
     4141 cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt
     4201 tatgcagagg ccgaggccgc ctctgcctct gagctattcc agaagtagtg aggaggcttt
     4261 tttggaggcc taggcttttg caaaaagctc ccgggagctt gtatatccat tttcggatct
     4321 gatcagcacg tgttgacaat taatcatcgg catagtatat cggcatagta taatacgaca
     4381 aggtgaggaa ctaaaccatg gccaagttga ccagtgccgt tccggtgctc accgcgcgcg
     4441 acgtcgccgg agcggtcgag ttctggaccg accggctcgg gttctcccgg gacttcgtgg
     4501 aggacgactt cgccggtgtg gtccgggacg acgtgaccct gttcatcagc gcggtccagg
     4561 accaggtggt gccggacaac accctggcct gggtgtgggt gcgcggcctg gacgagctgt
     4621 acgccgagtg gtcggaggtc gtgtccacga acttccggga cgcctccggg ccggccatga
     4681 ccgagatcgg cgagcagccg tgggggcggg agttcgccct gcgcgacccg gccggcaact
     4741 gcgtgcactt cgtggccgag gagcaggact gacacgtgct acgagatttc gattccaccg
     4801 ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc tggatgatcc
     4861 tccagcgcgg ggatctcatg ctggagttct tcgcccaccc caacttgttt attgcagctt
     4921 ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac
     4981 tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tgtataccgt
     5041 cgacctctag ctagagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt
     5101 atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg
     5161 cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg
     5221 gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc
     5281 gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
     5341 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
     5401 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
     5461 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
     5521 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
     5581 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
     5641 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
     5701 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
     5761 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
     5821 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
     5881 tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc
     5941 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
     6001 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
     6061 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
     6121 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
     6181 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
     6241 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
     6301 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
     6361 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag
     6421 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
     6481 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
     6541 ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg
     6601 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct
     6661 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta
     6721 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
     6781 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
     6841 cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg
     6901 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
     6961 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
     7021 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
     7081 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
     7141 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
     7201 catttccccg aaaagtgcca cctgacgtcg acggatcggg agatctcccg atcccctatg
     7261 gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtatc tgctccctgc
     7321 ttgtgtgttg gaggtcgctg agtagtgcgc gagcaaaatt taagctacaa caaggcaagg
     7381 cttgaccgac aattgcatga agaatctgct tagggttagg cgttttgcgc tgcttcgcga
     7441 tgtacgggcc agatatacgc gttgacattg attattgact agttattaat agtaatcaat
     7501 tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac ttacggtaaa
     7561 tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt
     7621 tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta
     7681 aactgcccac ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt
     7741 caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttat gggactttcc
     7801 tacttggcag tacatctacg tattagtcat cgctattacc atggtgatgc ggttttggca
     7861 gtacatcaat gggcgtggat agcggtttga ctcacgggga tttccaagtc tccaccccat
     7921 tgacgtcaat gggagtttgt tttggcacca aaatcaacgg gactttccaa aatgtcgtaa
     7981 caactccgcc ccattgacgc aaatgggcgg taggcgtgta cggtgggagg tctatataag
     8041 cagcgcgttt tgcctgtact gggtctctct ggttagacca gatctgagcc tgggagctct
     8101 ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag
     8161 tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt
     8221 cagtgtggaa aatctctagc agtggcgccc gaacagggac ttgaaagcga aagggaaacc
     8281 agaggagctc tctcgacgca ggactcggct tgctgaagcg cgcacggcaa gaggcgaggg
     8341 gcggcgactg gtgagtacgc caaaaatttt gactagcgga ggctagaagg agagagatgg
     8401 gtgcgagagc gtcagtatta agcgggggag aattagatcg cgatgggaaa aaattcggtt
     8461 aaggccaggg ggaaagaaaa aatataaatt aaaacatata gtatgggcaa gcagggagct
     8521 agaacgattc gcagttaatc ctggcctgtt agaaacatca gaaggctgta gacaaatact
     8581 gggacagcta caaccatccc ttcagacagg atcagaagaa cttagatcat tatataatac
     8641 agtagcaacc ctctattgtg tgcatcaaag gatagagata aaagacacca aggaagcttt
     8701 agacaagata gaggaagagc aaaacaaaag taagaccacc gcacagcaag cggccgctga
     8761 tcttcagacc tggaggagga gatatgaggg acaattggag aagtgaatta tataaatata
     8821 aagtagtaaa aattgaacca ttaggagtag cacccaccaa ggcaaagaga agagtggtgc
     8881 agagagaaaa aagagcagtg ggaataggag ctttgttcct tgggttcttg ggagcagcag
     8941 gaagcactat gggcgcagcg tcaatgacgc tgacggtaca ggccagacaa ttattgtctg
     9001 gtatagtgca gcagcagaac aatttgctga gggctattga ggcgcaacag catctgttgc
     9061 aactcacagt ctggggcatc aagcagctcc aggcaagaat cctggctgtg gaaagatacc
     9121 taaaggatca acagctcctg gggatttggg gttgctctgg aaaactcatt tgcaccactg
     9181 ctgtgccttg gaatgctagt tggagtaata aatctctgga acagatttgg aatcacacga
     9241 cctggatgga gtgggacaga gaaattaaca attacacaag cttaatacac tccttaattg
     9301 aagaatcgca aaaccagcaa gaaaagaatg aacaagaatt attggaatta gataaatggg
     9361 caagtttgtg gaattggttt aacataacaa attggctgtg gtatataaaa ttattcataa
     9421 tgatagtagg aggcttggta ggtttaagaa tagtttttgc tgtactttct atagtgaata
     9481 gagttaggca gggatattca ccattatcgt ttcagaccca cctcccaacc ccgaggggac
     9541 ccgacaggcc cgaaggaata gaagaagaag gtggagagag agacagagac agatccattc
     9601 gattagtgaa cggatcggca ctgcgtgcgc caattctgca gacaaatggc agtattcatc
     9661 cacaatttta aaagaaaagg ggggattggg gggtacagtg caggggaaag aatagtagac
     9721 ataatagcaa cagacataca aactaaagaa ttacaaaaac aaattacaaa aattcaaaat
     9781 tttcgggttt attacaggga cagcagagat ccagtttggt taattaagaa aggatccgaa
     9841 agaattcgcc cttagatctc tagacaaaaa agagataata agcttgtcta tctcttgaat
     9901 agacaagctt attatctcgg gaaagagtgg tctcatacag aacttataag attcccaaat
     9961 ccaaagacat ttcacgttta tggtgatttc ccagaacaca tagcgacatg caaatattgc
    10021 agggcgccac tcccctgtcc ctcacagcca tcttcctgcc agggcgcacg cgcgctgggt
    10081 gttcccgcct agtgacactg ggcccgcgat tccttggagc gggttgatga cgtcagcgtt
    10141 cttaattaac ccgtgtcggc tccagatct
//
