LOCUS       sequence-139475-       11791 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11791)
  AUTHORS   Fasano CA, Dimos JT, Ivanova NB, Lowry N, Lemischka IR, Temple S
  TITLE     shRNA knockdown of Bmi-1 reveals a critical role for p21-Rb pathway 
            in NSC self-renewal during development.
  JOURNAL   Cell Stem Cell. 2007 Jun 7. 1(1):87-99.
  PUBMED    18371338
REFERENCE   2  (bases 1 to 11791)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Stem 
            Cell. 2007 Jun 7. 1(1):87-99."
COMMENT     Sequence Label: Bmi1-overexpression
FEATURES             Location/Qualifiers
     source          1..11791
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..1212
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     1049..1068
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     misc_feature    join(2515..3089,3090..3092,3093..3101)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(2682..2699)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     2909..2928
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(3102..3104,3105..3107,3108..3821)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(3102..3104,3105..3107,3108..3818)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3147..3168)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3408..3427)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3755..3776
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    3862..4450
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3915..3935)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4333..4344)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(4453..4469)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4454..4470)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             4975..5155
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(5181..5198)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5187..5411
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5457..5885
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5544..5563)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5754..5775
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5894..5914)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5899..6228
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6079..6214
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6141..6160
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        6276..6323
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             6342..6716
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    6846..6967
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6883..6902)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6937..6956
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7016..7032)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7016..7032)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7029..7051)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7040..7056
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7064..7070,7071..7088,7089..7094))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7109..7130
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7247..7264)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7418..8006)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7498..7517)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8177..8968,8969..9037))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8800..8819
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9038..9142)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(9217..9236)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        9408..9787
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        9789..9987
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     9942..9962
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             10005..10185
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    10232..10357
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    10850..11083
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             11268..11312
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    11610..11727
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggcctccgcg ccgggttttg gcgcctcccg cgggcgcccc cctcctcacg gcgagcgctg
       61 ccacgtcaga cgaagggcgc agcgagcgtc ctgatccttc cgcccggacg ctcaggacag
      121 cggcccgctg ctcataagac tcggccttag aaccccagta tcagcagaag gacattttag
      181 gacgggactt gggtgactct agggcactgg ttttctttcc agagagcgga acaggcgagg
      241 aaaagtagtc ccttctcggc gattctgcgg agggatctcc gtggggcggt gaacgccgat
      301 gattatataa ggacgcgccg ggtgtggcac agctagttcc gtcgcagccg ggatttgggt
      361 cgcggttctt gtttgtggat cgctgtgatc gtcacttggt gagtagcggg ctgctgggct
      421 ggccggggct ttcgtggccg ccgggccgct cggtgggacg gaagcgtgtg gagagaccgc
      481 caagggctgt agtctgggtc cgcgagcaag gttgccctga actgggggtt ggggggagcg
      541 cagcaaaatg gcggctgttc ccgagtcttg aatggaagac gcttgtgagg cgggctgtga
      601 ggtcgttgaa acaaggtggg gggcatggtg ggcggcaaga acccaaggtc ttgaggcctt
      661 cgctaatgcg ggaaagctct tattcgggtg agatgggctg gggcaccatc tggggaccct
      721 gacgtgaagt ttgtcactga ctggagaact cggtttgtcg tctgttgcgg gggcggcagt
      781 tatggcggtg ccgttgggca gtgcacccgt acctttggga gcgcgcgccc tcgtcgtgtc
      841 gtgacgtcac ccgttctgtt ggcttataat gcagggtggg gccacctgcc ggtaggtgtg
      901 cggtaggctt ttctccgtcg caggacgcag ggttcgggcc tagggtaggc tctcctgaat
      961 cgacaggcgc cggacctctg gtgaggggag ggataagtga ggcgtcagtt tctttggtcg
     1021 gttttatgta cctatcttct taagtagctg aagctccggt tttgaactat gcgctcgggg
     1081 ttggcgagtg tgttttgtga agttttttag gcaccttttg aaatgtaatc atttgggtca
     1141 atatgtaatt ttcagtgtta gactagtaaa ttgtccgcta aattctggcc gtttttggct
     1201 tttttgttag acgaagcttg ggctgcaggt cgactctaga ggatctcgag ctcaagcttc
     1261 gaattctgca gtcgacaaga ggtaatccac gacttttaac tttcattgtc ttttccgccc
     1321 gctcagatcg cctcgcgccg gccgctcttt ccgggatctt ttatcaagca gaaatgcatc
     1381 gaacaaccag aatcaagatc actgagctaa atccccactt aatgtgtgtc ctgtgtggag
     1441 ggtacttcat tgatgccact accataatag aatgtctaca ttccttctgt aaaacatgta
     1501 ttgtacgtta cttggagacc agcaagtatt gtcctatttg tgatgtccag gttcacaaaa
     1561 ccagaccact cctgaacata aggtcagata aaactcttca agatattgta tacaaattag
     1621 tcccagggct tttcaaaaat gagatgaaga gaagaagaga tttttatgca gctcacccgt
     1681 cagctgatgc tgccaatggc tccaatgaag accgaggaga agttgcggat gaggagaaga
     1741 ggattataac tgatgatgag ataataagct tgtctattga gttctttgat cagagcagat
     1801 tggatcggaa agtaaataaa gagaagccta aggaagaggt gaatgataaa aggtacttac
     1861 gatgcccagc agcaatgact gtgatgcact tgagaaagtt tctcaggagt aaaatggaca
     1921 tacccaatac tttccagatt gacgtcatgt atgaagagga acctttaaag gattactaca
     1981 cgctaatgga cattgcctac atttatacct ggagaagaaa tggcccacta cctttgaaat
     2041 acagagttcg gccaacttgc aaaagaatga agatgagtca ccagagggat ggactgacga
     2101 atgctggaga gctggaaagt gactctggga gtgacaaggc caacagccca gccggaggtg
     2161 ttccctccac ctcttcctgt ttgcccagtc ccagcactcc agtgcagtct cctcacccac
     2221 agttccctca catttccagt accatgaatg gaaccagcaa cagccccagt gctaaccacc
     2281 aatcttcctt tgccagtaga cctcgaaaat catcactaaa tgggtcatca gcaacttcat
     2341 ctggttagga ctgttaagga aaagattttt caaccccctg atttagttac cttcattcat
     2401 tacagcttta tagatgctta atacatgtga ctgtcgtcca gtttgcttcc ttttgtagtg
     2461 actttaaatt tggccataaa tgatggacta gatgtgatac ttcataggga tccgcccctc
     2521 tccctccccc ccccctaacg ttactggccg aagccgcttg gaataaggcc ggtgtgcgtt
     2581 tgtctatatg ttattttcca ccatattgcc gtcttttggc aatgtgaggg cccggaaacc
     2641 tggccctgtc ttcttgacga gcattcctag gggtctttcc cctctcgcca aaggaatgca
     2701 aggtctgttg aatgtcgtga aggaagcagt tcctctggaa gcttcttgaa gacaaacaac
     2761 gtctgtagcg accctttgca ggcagcggaa ccccccacct ggcgacaggt gcctctgcgg
     2821 ccaaaagcca cgtgtataag atacacctgc aaaggcggca caaccccagt gccacgttgt
     2881 gagttggata gttgtggaaa gagtcaaatg gctctcctca agcgtattca acaaggggct
     2941 gaaggatgcc cagaaggtac cccattgtat gggatctgat ctggggcctc ggtacacatg
     3001 ctttacatgt gtttagtcga ggttaaaaaa acgtctaggc cccccgaacc acggggacgt
     3061 ggttttcctt tgaaaaacac gatgataata tggccacaac catggtgagc aagggcgagg
     3121 agctgttcac cggggtggtg cccatcctgg tcgagctgga cggcgacgta aacggccaca
     3181 agttcagcgt gtccggcgag ggcgagggcg atgccaccta cggcaagctg accctgaagt
     3241 tcatctgcac caccggcaag ctgcccgtgc cctggcccac cctcgtgacc accctgacct
     3301 acggcgtgca gtgcttcagc cgctaccccg accacatgaa gcagcacgac ttcttcaagt
     3361 ccgccatgcc cgaaggctac gtccaggagc gcaccatctt cttcaaggac gacggcaact
     3421 acaagacccg cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc atcgagctga
     3481 agggcatcga cttcaaggag gacggcaaca tcctggggca caagctggag tacaactaca
     3541 acagccacaa cgtctatatc atggccgaca agcagaagaa cggcatcaag gtgaacttca
     3601 agatccgcca caacatcgag gacggcagcg tgcagctcgc cgaccactac cagcagaaca
     3661 cccccatcgg cgacggcccc gtgctgctgc ccgacaacca ctacctgagc acccagtccg
     3721 ccctgagcaa agaccccaac gagaagcgcg atcacatggt cctgctggag ttcgtgaccg
     3781 ccgccgggat cactctcggc atggacgagc tgtacaagta aagcggccgc gactctagaa
     3841 ttcgatatca agcttatcga taatcaacct ctggattaca aaatttgtga aagattgact
     3901 ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg
     3961 tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg
     4021 ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg
     4081 tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct cctttccggg
     4141 actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc
     4201 tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaaatca
     4261 tcgtcctttc cttggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc
     4321 tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct
     4381 ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga gtcggatctc cctttgggcc
     4441 gcctccccgc atcgataccg tcgacctcga gacctagaaa aacatggagc aatcacaagt
     4501 agcaatacag cagctaccaa tgctgattgt gcctggctag aagcacaaga ggaggaggag
     4561 gtgggttttc cagtcacacc tcaggtacct ttaagaccaa tgacttacaa ggcagctgta
     4621 gatcttagcc actttttaaa agaaaagggg ggactggaag ggctaattca ctcccaacga
     4681 agacaagata tccttgatct gtggatctac cacacacaag gctacttccc tgattggcag
     4741 aactacacac cagggccagg gatcagatat ccactgacct ttggatggtg ctacaagcta
     4801 gtaccagttg agcaagagaa ggtagaagaa gccaatgaag gagagaacac ccgcttgtta
     4861 caccctgtga gcctgcatgg gatggatgac ccggagagag aagtattaga gtggaggttt
     4921 gacagccgcc tagcatttca tcacatggcc cgagagctgc atccggactg tactgggtct
     4981 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
     5041 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
     5101 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagggcc
     5161 cgtttaaacc cgctgatcag cctcgactgt gccttctagt tgccagccat ctgttgtttg
     5221 cccctccccc gtgccttcct tgaccctgga aggtgccact cccactgtcc tttcctaata
     5281 aaatgaggaa attgcatcgc attgtctgag taggtgtcat tctattctgg ggggtggggt
     5341 ggggcaggac agcaaggggg aggattggga agacaatagc aggcatgctg gggatgcggt
     5401 gggctctatg gcttctgagg cggaaagaac cagctggggc tctagggggt atccccacgc
     5461 gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac
     5521 acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt
     5581 cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc
     5641 tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc
     5701 gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact
     5761 cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg
     5821 gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc
     5881 gaattaattc tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg ctccccagca
     5941 ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca
     6001 ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc
     6061 ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc
     6121 catggctgac taattttttt tatttatgca gaggccgagg ccgcctctgc ctctgagcta
     6181 ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaaa gctcccggga
     6241 gcttgtatat ccattttcgg atctgatcag cacgtgttga caattaatca tcggcatagt
     6301 atatcggcat agtataatac gacaaggtga ggaactaaac catggccaag ttgaccagtg
     6361 ccgttccggt gctcaccgcg cgcgacgtcg ccggagcggt cgagttctgg accgaccggc
     6421 tcgggttctc ccgggacttc gtggaggacg acttcgccgg tgtggtccgg gacgacgtga
     6481 ccctgttcat cagcgcggtc caggaccagg tggtgccgga caacaccctg gcctgggtgt
     6541 gggtgcgcgg cctggacgag ctgtacgccg agtggtcgga ggtcgtgtcc acgaacttcc
     6601 gggacgcctc cgggccggcc atgaccgaga tcggcgagca gccgtggggg cgggagttcg
     6661 ccctgcgcga cccggccggc aactgcgtgc acttcgtggc cgaggagcag gactgacacg
     6721 tgctacgaga tttcgattcc accgccgcct tctatgaaag gttgggcttc ggaatcgttt
     6781 tccgggacgc cggctggatg atcctccagc gcggggatct catgctggag ttcttcgccc
     6841 accccaactt gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt
     6901 tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg
     6961 tatcttatca tgtctgtata ccgtcgacct ctagctagag cttggcgtaa tcatggtcat
     7021 agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa
     7081 gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc
     7141 gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc
     7201 aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact
     7261 cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac
     7321 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa
     7381 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
     7441 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
     7501 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
     7561 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac
     7621 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
     7681 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
     7741 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
     7801 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa
     7861 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct
     7921 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga
     7981 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg
     8041 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct
     8101 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt
     8161 aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc
     8221 tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg
     8281 gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag
     8341 atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt
     8401 tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag
     8461 ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt
     8521 ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca
     8581 tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg
     8641 ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat
     8701 ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta
     8761 tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca
     8821 gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct
     8881 taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat
     8941 cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa
     9001 agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt
     9061 gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa
     9121 ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac gtcgacggat
     9181 cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg atgccgcata
     9241 gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt gcgcgagcaa
     9301 aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc tgcttagggt
     9361 taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac attgattatt
     9421 gactagttat taatagtaat caattacggg gtcattagtt catagcccat atatggagtt
     9481 ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg acccccgccc
     9541 attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt tccattgacg
     9601 tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat
     9661 gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc attatgccca
     9721 gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat
     9781 taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt ttgactcacg
     9841 gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc accaaaatca
     9901 acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg gcggtaggcg
     9961 tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct ctctggttag
    10021 accagatctg agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat
    10081 aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact
    10141 agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag
    10201 ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc ggcttgctga
    10261 agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa ttttgactag
    10321 cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg ggagaattag
    10381 atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata aattaaaaca
    10441 tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc tgttagaaac
    10501 atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga caggatcaga
    10561 agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc aaaggataga
    10621 gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca aaagtaagac
    10681 caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg agggacaatt
    10741 ggagaagtga attatataaa tataaagtag taaaaattga accattagga gtagcaccca
    10801 ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata ggagctttgt
    10861 tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg
    10921 tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg ctgagggcta
    10981 ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag ctccaggcaa
    11041 gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt tggggttgct
    11101 ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt aataaatctc
    11161 tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt aacaattaca
    11221 caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag aatgaacaag
    11281 aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata acaaattggc
    11341 tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta agaatagttt
    11401 ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta tcgtttcaga
    11461 cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa gaaggtggag
    11521 agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt gcgccaattc
    11581 tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat tggggggtac
    11641 agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa agaattacaa
    11701 aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag agatccagtt
    11761 tggttaatta acccgtgtcg gctccagatc t
//
