LOCUS       sequence-139491-         625 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Tol2 vector with chimeric endothelial enhancer/promoter upstream of 
            attR1/R2 cassette and late SV40 pA signal.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 625)
  AUTHORS   Villefranc JA, Amigo J, Lawson ND
  TITLE     Gateway compatible vectors for analysis of gene function in the 
            zebrafish.
  JOURNAL   Dev Dyn. 2007 Nov . 236(11):3077-87.
  PUBMED    17948311
REFERENCE   2  (bases 1 to 625)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Dev Dyn. 
            2007 Nov . 236(11):3077-87."
COMMENT     Sequence Label: 343 pTol2-fli1epDest
FEATURES             Location/Qualifiers
     source          1..625
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(join(81..87,88..105,106..111))
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    complement(136..260)
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
ORIGIN
        1 ggtatatcca gtgatttttt tctccatttt agcttcctta gctcctgaaa atctcgacgg
       61 atcctaactc aaaatccaca cattatacga gccggaagca taaagtgtaa agcctggggt
      121 gcctaatgcg gccgccatag tgactggata tgttgtgttt tacagtatta tgtagtctgt
      181 tttttatgca aaatctaatt taatatattg atatttatat cattttacgt ttctcgttca
      241 gcttttttgt acaaacttgt tgatccttga attcgaatcg atgctagcga taattcgccc
      301 ttcgcgtctg aattaattcc agcccccgat atgtggcgga gagacagaga cagtttcgcc
      361 ggatggtttt ttttcctcta aatttgggaa gtgaagtcac tgcgcttgag aaggaagccg
      421 tgtatgcgtg tatgtgtgtg tgtgtatgtg tgtgtgtcac tggccctgcc ttcattacgg
      481 cacccaaagc aggatatttg tccagcgaag ataagacacc aaaacaatgg tgtttctttc
      541 ctttgtgcat gtttttgcct ggaaatattt acagaatgcc tgccaactct ttacatttgt
      601 tgcgcttttt ttccgacatt aaaca
//
