LOCUS       sequence-139493-         654 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Tol2 vector with fli1ep element upstream of EGFP, attR1/R2 cassette,
            and late SV40 pA signal.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 654)
  AUTHORS   Villefranc JA, Amigo J, Lawson ND
  TITLE     Gateway compatible vectors for analysis of gene function in the 
            zebrafish.
  JOURNAL   Dev Dyn. 2007 Nov . 236(11):3077-87.
  PUBMED    17948311
REFERENCE   2  (bases 1 to 654)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Dev Dyn. 
            2007 Nov . 236(11):3077-87."
COMMENT     Sequence Label: 372 pTol2-fli1ep:EGFPDest
FEATURES             Location/Qualifiers
     source          1..654
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(join(72..78,79..96,97..102))
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    complement(127..251)
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(315..336)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 agtgattttt ttctccattt tagcttcctt agctcctgaa aatctcgacg gatcctaact
       61 caaaatccac acattatacg agccggaagc ataaagtgta aagcctgggg tgcctaatgc
      121 ggccgccata gtgactggat atgttgtgtt ttacagtatt atgtagtctg ttttttatgc
      181 aaaatctaat ttaatatatt gatatttata tcattttacg tttctcgttc agcttttttg
      241 tacaaacttg tgattcgaga tctgagtccg gacttgtaca gctcgtccat gccgagagtg
      301 atcccggcgg cggtcacgaa ctccagcagg accatgtgat cgcgcttctc gttggggtct
      361 ttgctcaggg cggactgggt gctcaggtag tggttgtcgg gcagcagcac ggggccgtcg
      421 ccgatggggg tgttctgctg gtagtggtcg gcgagctgca cgctgccgtc ctcgatgttg
      481 tggcggatct tgaagttcac cttgatgccg ttcttctgct tgtcggccat gatatagacg
      541 ttgtggctgt tgtagttgta ctccagcttg tgccccagga tgttgccgtc ctccttgaag
      601 tcgatgccct tcagctcgat gcggttcacc agggtgtcgc cctcgaactt cacc
//
