LOCUS       sequence-139494-         703 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Tol2 vector with fli1ep element upstream of mCherry, attR1/R2 
            cassette, and late SV40 pA signal.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 703)
  AUTHORS   Villefranc JA, Amigo J, Lawson ND
  TITLE     Gateway compatible vectors for analysis of gene function in the 
            zebrafish.
  JOURNAL   Dev Dyn. 2007 Nov . 236(11):3077-87.
  PUBMED    17948311
REFERENCE   2  (bases 1 to 703)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Dev Dyn. 
            2007 Nov . 236(11):3077-87."
COMMENT     Sequence Label: 373 pTol2-fli1ep:CherryDest
FEATURES             Location/Qualifiers
     source          1..703
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(join(81..87,88..105,106..111))
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    complement(136..260)
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(350..373)
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     primer_bind     complement(548..567)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 ggtatatcca gtgatttttt tctccatttt agcttcctta gctcctgaaa atctcgacgg
       61 atcctaactc aaaatccaca cattatacga gccggaagca taaagtgtaa agcctggggt
      121 gcctaatgcg gccgccatag tgactggata tgttgtgttt tacagtatta tgtagtctgt
      181 tttttatgca aaatctaatt taatatattg atatttatat cattttacgt ttctcgttca
      241 gcttttttgt acaaacttgt gattcgagat ctcttgtaca gctcgtccat gccgccggtg
      301 gagtggcggc cctcggcgcg ctcgtactgt tccacgatgg tgtagtcctc gttgtgggag
      361 gtgatgtcca gcttgatgtt gacgttgtag gcgccgggca gctgcacggg cttcttggcc
      421 ttgtaggtgg tcttgacctc ggcgtcgtag tggccgccgt ccttcagctt cagcctctgc
      481 ttgatctcgc ccttcagggc gccgtcctcg gggtacatcc gctcggagga ggcctcccag
      541 cccatggtct tcttctgcat tacggggccg tcggagggga agttggtgcc gcgcagcttc
      601 accttgtaga tgaactcgcc gtcctgcagg gaggagtcct gggtcacggt caccacgccg
      661 ccgtcctcga agttcatcac gcgctcccac ttgaagccct cgg
//
