LOCUS       sequence-139497-        1065 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  SapTrap destination vector for building sgRNA expression vectors. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1065)
  AUTHORS   Schwartz ML, Jorgensen EM
  TITLE     SapTrap, a Toolkit for High-Throughput CRISPR/Cas9 Gene Modification
            in Caenorhabditis elegans.
  JOURNAL   Genetics. 2016 Feb 2. pii: genetics.115.184275.
  PUBMED    26837755
REFERENCE   2  (bases 1 to 1065)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genetics. 
            2016 Feb 2. pii: genetics.115.184275."
COMMENT     Sequence Label: pMLS134
FEATURES             Location/Qualifiers
     source          1..1065
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_RNA        507..582
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     misc_feature    590..826
                     /label=3' CeU6
                     /note="region downstream of the C. elegans K09B11.12 U6 
                     snRNA gene"
     primer_bind     complement(845..861)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(845..861)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(858..880)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    869..885
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(893..899,900..917,918..923))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    938..959
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 tgctctgaag taggtctcga gatcaatact gggaagacga aagtcttgcg aaaccgattc
       61 gctgacccca gtaaagtcta cttcggtagc ccttccccca ccacccagct cgacgacgtc
      121 gacgagtaca tctacctcgg tcgtcaaatc aacgcccaaa acaacttgat gccggaaatc
      181 caccgaagac gtcgagcagc ctgggctgca ttcaatggaa tcaagaatgc caccgactcc
      241 atcaccgaca agaagattcg tgcgaatctg ttcgactcaa ttgtccttcc agcgctcacc
      301 tacgtttcag aagcctggac attcaccaaa gctctatccg aacgagtacg aatcacacat
      361 gcctccccca caaattattg atctacatcc ccatttggta tagtgtctta caggactctc
      421 aatatgtcag ctgcatgata aatggtccct atataattga attgcaaatc taaatgtttg
      481 cgaagagcgg atccatgggc tcttcggttt tagagctaga aatagcaagt taaaataagg
      541 ctagtccgtt atcaacttga aaaagtggca ccgagtcggt gctttttttc aataatattc
      601 tagatatccc tttttgaaac taaaatgaaa tactctaaat ttttgagttc aatgtttcaa
      661 ctaatcttgg ttaaaggtaa attcccggag ggacgatttt aaagttgcca gagttgatta
      721 aattggtcca aaaaatttta ttaggaaact cgaaaaaaaa ttgatttttt ttgacaatat
      781 tccaaagaaa attacaaaat ctcgacgcca aacatagtcg gctgtgaagc ttggcgtaat
      841 catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac
      901 gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa
      961 ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat
     1021 gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgct
//
