LOCUS       sequence-139499-        1024 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  SapTrap destination vector for building sgRNA expression vectors. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1024)
  AUTHORS   Schwartz ML, Jorgensen EM
  TITLE     SapTrap, a Toolkit for High-Throughput CRISPR/Cas9 Gene Modification
            in Caenorhabditis elegans.
  JOURNAL   Genetics. 2016 Feb 2. pii: genetics.115.184275.
  PUBMED    26837755
REFERENCE   2  (bases 1 to 1024)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genetics. 
            2016 Feb 2. pii: genetics.115.184275."
COMMENT     Sequence Label: pMLS134
FEATURES             Location/Qualifiers
     source          1..1024
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_RNA        complement(231..306)
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        complement(334..833)
                     /label=CeU6 promoter
                     /note="promoter for the C. elegans K09B11.12 U6 snRNA gene 
                     (Friedland et al., 2013)"
     primer_bind     complement(840..857)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(840..856)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(849..871)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 tttggcgtcg agattttgta attttctttg gaatattgtc aaaaaaaatc aatttttttt
       61 cgagtttcct aataaaattt tttggaccaa tttaatcaac tctggcaact ttaaaatcgt
      121 ccctccggga atttaccttt aaccaagatt agttgaaaca ttgaactcaa aaatttagag
      181 tatttcattt tagtttcaaa aagggatatc tagaatatta ttgaaaaaaa gcaccgactc
      241 ggtgccactt tttcaagttg ataacggact agccttattt taacttgcta tttctagctc
      301 taaaaccgaa gagcccatgg atccgctctt cgcaaacatt tagatttgca attcaattat
      361 atagggacca tttatcatgc agctgacata ttgagagtcc tgtaagacac tataccaaat
      421 ggggatgtag atcaataatt tgtgggggag gcatgtgtga ttcgtactcg ttcggataga
      481 gctttggtga atgtccaggc ttctgaaacg taggtgagcg ctggaaggac aattgagtcg
      541 aacagattcg cacgaatctt cttgtcggtg atggagtcgg tggcattctt gattccattg
      601 aatgcagccc aggctgctcg acgtcttcgg tggatttccg gcatcaagtt gttttgggcg
      661 ttgatttgac gaccgaggta gatgtactcg tcgacgtcgt cgagctgggt ggtgggggaa
      721 gggctaccga agtagacttt actggggtca gcgaatcggt ttcgcaagac tttcgtcttc
      781 ccagtattga tctcgagacc tacttcagag catttttgta cgagttcttg gaggaattca
      841 ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc
      901 cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc
      961 ccttcccaac agttgcgcag cctgaatggc gaatggcgcc tgatgcggta ttttctcctt
     1021 acgc
//
