LOCUS       sequence-139500-         868 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Hairpin targeting GFP..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 868)
  AUTHORS   Jensen KJ, Moyer CB, Janes KA
  TITLE     Network Architecture Predisposes an Enzyme to Either Pharmacologic 
            or Genetic Targeting.
  JOURNAL   Cell Syst. 2016 Feb 24;2(2):112-121.
  PUBMED    26942229
REFERENCE   2  (bases 1 to 868)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Syst."; date: "2016-02-24"; volume: "2(2)"; pages: "112-121"
COMMENT     Sequence Label: shGFP neo
FEATURES             Location/Qualifiers
     source          1..868
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        69..317
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     69..89
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     240..259
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_feature    416..533
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ttcgattagt gaacggatct cgacggtatc gatcacgaga ctagcctcga gcggccgccc
       61 ccttcaccga gggcctattt cccatgattc cttcatattt gcatatacga tacaaggctg
      121 ttagagagat aattggaatt aatttgactg taaacacaaa gatattagta caaaatacgt
      181 gacgtagaaa gtaataattt cttgggtagt ttgcagtttt aaaattatgt tttaaaatgg
      241 actatcatat gcttaccgta acttgaaagt atttcgattt cttggcttta tatatcttgt
      301 ggaaaggacg aaacaccggg caagctgacc ctgaagttca tctgcagatg aacttcaggg
      361 tcagcttgct ttttgaattc tcgacctcga gacaaatggc agtattcatc cacaatttta
      421 aaagaaaagg ggggattggg gggtacagtg caggggaaag aatagtagac ataatagcaa
      481 cagacataca aactaaagaa ttacaaaaac aaattacaaa aattcaaaat tttcgggttt
      541 attacaggga cagcagagat ccactttggc cgcggctcga gggggttggg gttgcgcctt
      601 ttccaaggca gccctgggtt tgcgcaggga cgcggctgct ctgggcgtgg ttccgggaaa
      661 cgcagcggcg ccgaccctgg gtctcgcaca ttcttcacgt ccgttcgcag cgtcacccgg
      721 atcttcgccg ctacccttgt gggccccccg gcgacgcttc ctgctccgcc cctaagtcgg
      781 gaaggttcct tgcggttcgc ggcgtgccgg acgtgacaaa cggaagccgc acgtctcact
      841 agtaccctcg cagacggaca gcgccagg
//
