LOCUS       sequence-139501-         954 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Hairpin targeting MEK1 and MEK2 (murine)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 954)
  AUTHORS   Jensen KJ, Moyer CB, Janes KA
  TITLE     Network Architecture Predisposes an Enzyme to Either Pharmacologic 
            or Genetic Targeting.
  JOURNAL   Cell Syst. 2016 Feb 24;2(2):112-121.
  PUBMED    26942229
REFERENCE   2  (bases 1 to 954)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Syst."; date: "2016-02-24"; volume: "2(2)"; pages: "112-121"
COMMENT     Sequence Label: shMEK1/2 neo
FEATURES             Location/Qualifiers
     source          1..954
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        126..374
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     126..146
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     297..316
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_feature    473..590
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ccgacaggcc cgaaggaata gaagaagaag gtggagagag agacagagac agatccattc
       61 gattagtgaa cggatctcga cggtatcgat cacgagacta gcctcgagcg gccgccccct
      121 tcaccgaggg cctatttccc atgattcctt catatttgca tatacgatac aaggctgtta
      181 gagagataat tggaattaat ttgactgtaa acacaaagat attagtacaa aatacgtgac
      241 gtagaaagta ataatttctt gggtagtttg cagttttaaa attatgtttt aaaatggact
      301 atcatatgct taccgtaact tgaaagtatt tcgatttctt ggctttatat atcttgtgga
      361 aaggacgaaa caccggccag atcatccggg agctgcactg cagtgcagct cccggatgat
      421 ctgggttttt tgaattctcg acctcgagac aaatggcagt attcatccac aattttaaaa
      481 gaaaaggggg gattgggggg tacagtgcag gggaaagaat agtagacata atagcaacag
      541 acatacaaac taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt
      601 acagggacag cagagatcca ctttggccgc ggctcgaggg ggttggggtt gcgccttttc
      661 caaggcagcc ctgggtttgc gcagggacgc ggctgctctg ggcgtggttc cgggaaacgc
      721 agcggcgccg accctgggtc tcgcacattc ttcacgtccg ttcgcagcgt cacccggatc
      781 ttcgccgcta cccttgtggg ccccccggcg acgcttcctg ctccgcccct aagtcgggaa
      841 ggttccttgc ggttcgcggc gtgccggacg tgacaaacgg aagccgcacg tctcactagt
      901 accctcgcag acggacagcg ccagggagca atggcagcgc gccgaccgcg atgg
//
