LOCUS       sequence-139503-        1062 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  SapTrap destination vector for building combined sgRNA expression + 
            repair template vectors.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1062)
  AUTHORS   Schwartz ML, Jorgensen EM
  TITLE     SapTrap, a Toolkit for High-Throughput CRISPR/Cas9 Gene Modification
            in Caenorhabditis elegans.
  JOURNAL   Genetics. 2016 Feb 2. pii: genetics.115.184275.
  PUBMED    26837755
REFERENCE   2  (bases 1 to 1062)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genetics. 
            2016 Feb 2. pii: genetics.115.184275."
COMMENT     Sequence Label: pMLS256
FEATURES             Location/Qualifiers
     source          1..1062
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     27..43
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     28..44
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(157..177)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(157..175)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(196..212)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(196..212)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(209..231)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    220..236
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(244..250,251..268,269..274))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    289..310
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        413..912
                     /label=CeU6 promoter
                     /note="promoter for the C. elegans K09B11.12 U6 snRNA gene 
                     (Friedland et al., 2013)"
     misc_RNA        967..1042
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
ORIGIN
        1 gggcgaattg ggtaccgggc cccccctcga ggtcgacggt atcgataagc ttgattaccg
       61 aagagcaggc ctgcagaatt ccatatgact agtggatcca tgggctcttc gccaatcgaa
      121 ttggccgcca ccgcggtgga gctccagctt ttgttccctt tagtgagggt taattgcgcg
      181 cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc
      241 acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta
      301 actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca
      361 gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggctccaaga
      421 actcgtacaa aaatgctctg aagtaggtct cgagatcaat actgggaaga cgaaagtctt
      481 gcgaaaccga ttcgctgacc ccagtaaagt ctacttcggt agcccttccc ccaccaccca
      541 gctcgacgac gtcgacgagt acatctacct cggtcgtcaa atcaacgccc aaaacaactt
      601 gatgccggaa atccaccgaa gacgtcgagc agcctgggct gcattcaatg gaatcaagaa
      661 tgccaccgac tccatcaccg acaagaagat tcgtgcgaat ctgttcgact caattgtcct
      721 tccagcgctc acctacgttt cagaagcctg gacattcacc aaagctctat ccgaacgagt
      781 acgaatcaca catgcctccc ccacaaatta ttgatctaca tccccatttg gtatagtgtc
      841 ttacaggact ctcaatatgt cagctgcatg ataaatggtc cctatataat tgaattgcaa
      901 atctaaatgt ttgcgaagag caggcctgca gaattccata tgactagtgg atccatgggc
      961 tcttcggttt tagagctaga aatagcaagt taaaataagg ctagtccgtt atcaacttga
     1021 aaaagtggca ccgagtcggt gctttttttc aataatattc ta
//
