LOCUS       sequence-139504-        1014 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  SapTrap destination vector for building combined sgRNA expression + 
            repair template vectors.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1014)
  AUTHORS   Schwartz ML, Jorgensen EM
  TITLE     SapTrap, a Toolkit for High-Throughput CRISPR/Cas9 Gene Modification
            in Caenorhabditis elegans.
  JOURNAL   Genetics. 2016 Feb 2. pii: genetics.115.184275.
  PUBMED    26837755
REFERENCE   2  (bases 1 to 1014)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genetics. 
            2016 Feb 2. pii: genetics.115.184275."
COMMENT     Sequence Label: pMLS256
FEATURES             Location/Qualifiers
     source          1..1014
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(123..139)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(124..140)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     complement(164..183)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(165..183)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(193..210)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(193..209)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(202..224)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      351..806
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(438..457)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     648..669
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        832..936
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tcactaaagg gaacaaaagc tggagctcca ccgcggtggc ggccaattcg attggcgaag
       61 agcccatgga tccactagtc atatggaatt ctgcaggcct gctcttcggt aatcaagctt
      121 atcgataccg tcgacctcga gggggggccc ggtacccaat tcgccctata gtgagtcgta
      181 ttacgcgcgc tcactggccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac
      241 ccaacttaat cgccttgcag cacatccccc tttcgccagc tggcgtaata gcgaagaggc
      301 ccgcaccgat cgcccttccc aacagttgcg cagcctgaat ggcgaatggg acgcgccctg
      361 tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc
      421 cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg
      481 ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg
      541 gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg
      601 atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt
      661 ccaaactgga acaacactca accctatctc ggtctattct tttgatttat aagggatttt
      721 gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt
      781 taacaaaata ttaacgctta caatttaggt ggcacttttc ggggaaatgt gcgcggaacc
      841 cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc
      901 tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc
      961 gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaa
//
