LOCUS       sequence-139507-        1127 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  SapTrap destination vector for building repair template only 
            vectors.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1127)
  AUTHORS   Schwartz ML, Jorgensen EM
  TITLE     SapTrap, a Toolkit for High-Throughput CRISPR/Cas9 Gene Modification
            in Caenorhabditis elegans.
  JOURNAL   Genetics. 2016 Feb 2. pii: genetics.115.184275.
  PUBMED    26837755
REFERENCE   2  (bases 1 to 1127)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genetics. 
            2016 Feb 2. pii: genetics.115.184275."
COMMENT     Sequence Label: pMLS257
FEATURES             Location/Qualifiers
     source          1..1127
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     27..43
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     28..44
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(176..196)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(176..194)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(215..231)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(215..231)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(228..250)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    239..255
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(263..269,270..287,288..293))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    308..329
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(439..456)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(690..709)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gggcgaattg ggtaccgggc cccccctcga ggtcgacggt atcgataagc ttgattaccg
       61 aagagcaggc ctgcagaatt ccatatgact agtggatcca tgggctcttc gccaatcgaa
      121 ttcctgcagc ccttctagag cggccgccac cgcggtggag ctccagcttt tgttcccttt
      181 agtgagggtt aattgcgcgc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt
      241 gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg
      301 gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt
      361 cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt
      421 tgcgtattgg gccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg
      481 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc
      541 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
      601 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
      661 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
      721 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
      781 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
      841 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
      901 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
      961 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
     1021 gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg ctctgctgaa
     1081 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc gcaacaa
//
