LOCUS       sequence-139508-        1059 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  SapTrap destination vector for building repair template only 
            vectors.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1059)
  AUTHORS   Schwartz ML, Jorgensen EM
  TITLE     SapTrap, a Toolkit for High-Throughput CRISPR/Cas9 Gene Modification
            in Caenorhabditis elegans.
  JOURNAL   Genetics. 2016 Feb 2. pii: genetics.115.184275.
  PUBMED    26837755
REFERENCE   2  (bases 1 to 1059)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genetics. 
            2016 Feb 2. pii: genetics.115.184275."
COMMENT     Sequence Label: pMLS257
FEATURES             Location/Qualifiers
     source          1..1059
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(142..158)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(143..159)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     complement(183..202)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(184..202)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(212..229)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(212..228)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(221..243)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      370..825
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(457..476)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     667..688
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        851..955
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tcactaaagg gaacaaaagc tggagctcca ccgcggtggc ggccgctcta gaagggctgc
       61 aggaattcga ttggcgaaga gcccatggat ccactagtca tatggaattc tgcaggcctg
      121 ctcttcggta atcaagctta tcgataccgt cgacctcgag ggggggcccg gtacccaatt
      181 cgccctatag tgagtcgtat tacgcgcgct cactggccgt cgttttacaa cgtcgtgact
      241 gggaaaaccc tggcgttacc caacttaatc gccttgcagc acatccccct ttcgccagct
      301 ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc agcctgaatg
      361 gcgaatggga cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca
      421 gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct
      481 ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt
      541 tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac
      601 gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct
      661 ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt
      721 ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac
      781 aaaaatttaa cgcgaatttt aacaaaatat taacgcttac aatttaggtg gcacttttcg
      841 gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc
      901 gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag
      961 tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt
     1021 tgctcaccca gaaacgctgg tgaaagtaaa agatgctga
//
