LOCUS       sequence-139510-         802 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Hairpin targeting MEK1 and MEK2 (murine).  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 802)
  AUTHORS   Jensen KJ, Moyer CB, Janes KA
  TITLE     Network Architecture Predisposes an Enzyme to Either Pharmacologic 
            or Genetic Targeting.
  JOURNAL   Cell Syst. 2016 Feb 24;2(2):112-121.
  PUBMED    26942229
REFERENCE   2  (bases 1 to 802)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Syst."; date: "2016-02-24"; volume: "2(2)"; pages: "112-121"
COMMENT     Sequence Label: shMEK1/2 puro
FEATURES             Location/Qualifiers
     source          1..802
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        126..374
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     126..146
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     297..316
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_feature    471..588
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ccgacaggcc cgaaggaata gaagaagaag gtggagagag agacagagac agatccattc
       61 gattagtgaa cggatctcga cggtatcgat cacgagacta gcctcgagcg gccgccccct
      121 tcaccgaggg cctatttccc atgattcctt catatttgca tatacgatac aaggctgtta
      181 gagagataat tggaattaat ttgactgtaa acacaaagat attagtacaa aatacgtgac
      241 gtagaaagta ataatttctt gggtagtttg cagttttaaa attatgtttt aaaatggact
      301 atcatatgct taccgtaact tgaaagtatt tcgatttctt ggctttatat atcttgtgga
      361 aaggacgaaa caccggccag atcatccggg agctgcactg cagtgcagct cccggatgat
      421 ctggtttttg aattctcgac ctcgagacaa atggcagtat tcatccacaa ttttaaaaga
      481 aaagggggga ttggggggta cagtgcaggg gaaagaatag tagacataat agcaacagac
      541 atacaaacta aagaattaca aaaacaaatt acaaaaattc aaaattttcg ggtttattac
      601 agggacagca gagatccact ttggccgcgg ctcgaggggg ttggggttgc gccttttcca
      661 aggcagccct gggtttgcgc agggacgcgg ctgctctggg cgtggttccg ggaaacgcag
      721 cggcgccgac cctgggtctc gcacattctt cacgtccgtt cgcagcgtca cccggatctt
      781 cgccgctacc cttgtgggcc cc
//
