LOCUS       sequence-139513-         976 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  C. elegans Pan-neuronal 2xNLS-FLP-D5 expression vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 976)
  AUTHORS   Schwartz ML, Jorgensen EM
  TITLE     SapTrap, a Toolkit for High-Throughput CRISPR/Cas9 Gene Modification
            in Caenorhabditis elegans.
  JOURNAL   Genetics. 2016 Feb 2. pii: genetics.115.184275.
  PUBMED    26837755
REFERENCE   2  (bases 1 to 976)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genetics. 
            2016 Feb 2. pii: genetics.115.184275."
COMMENT     Sequence Label: pMLS262
FEATURES             Location/Qualifiers
     source          1..976
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    437..461
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(465..536)
                     /codon_start=1
                     /product="nuclear localization signal from the C. elegans 
                     EGL-13 transcription factor (Lyssenko et al., 2007)"
                     /label=EGL-13 NLS
                     /translation="SRRRKANPTKLSENAKKLAKEVEN"
ORIGIN
        1 gttgccaagc gaggacaatt ctcatcgttc gcctaatttc tttgcttatt ttttactagt
       61 tttccttcct cctctatata tttttatgtt ttcttttaaa ccttcctcca tatttctgaa
      121 ttgaatgttg ttgcatattg tgtgtcctta tttcatttcc atcatccttt tgtgtgtttc
      181 ccccttccac caattccccc cacgtcctaa tgaaatgaaa gcgagaagat aaataaactt
      241 gcacattgtg ttcagatgag aggagcggaa tacgaattgg gagacggaaa gagaaaaaaa
      301 aatgtttaga tttggattga attaattttt tagaaagcaa gaaaaaaaac acaaagagaa
      361 taaataaata ataggaaaaa aaacaaatat tcagtattta aaatttgaaa attcaacgac
      421 gttggcgtcg atcatcccca ctttgtacaa gaaagctggg tttagttctc gacctccttg
      481 gcgagcttct tggcgttctc ggagagcttg gttgggttgg ccttacgacg acgggagata
      541 cgacggttga tgtaggagga gaggtagtcg aggacctctt gggagatgat tccgttccag
      601 gctgggtaac ggatggatcc ctcggcggaa cccttgagtt gctcgatgtg ttgccactcc
      661 tcgattgggt tggtctcgtc cttgagggcg atcatctcct tggagattgg gtcgtaggcg
      721 tagtaacggg agacgagggc gaagtagtgg tctgggatgg cggtgatttg gtgggtgtag
      781 gtggtacggg cgacggcgga ggcacgcttg tcggaccagt ttccgacgac gttggtgagc
      841 tcggtgagtc ccttcatgga gaggaaggag gtcatgaggt gacgtccgat gtgggacttt
      901 ggtccgttct tgatggcgaa gatggagtat ggggcgttct tcttgagggc ctgaaaattt
      961 aaatatgtat ggttag
//
