LOCUS       sequence-139518-        1006 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  SapTrap Single plasmid targeting vector for tagging Unc-32 with GFP 
            by CRISPR/Cas9.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1006)
  AUTHORS   Schwartz ML, Jorgensen EM
  TITLE     SapTrap, a Toolkit for High-Throughput CRISPR/Cas9 Gene Modification
            in Caenorhabditis elegans.
  JOURNAL   Genetics. 2016 Feb 2. pii: genetics.115.184275.
  PUBMED    26837755
REFERENCE   2  (bases 1 to 1006)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genetics. 
            2016 Feb 2. pii: genetics.115.184275."
COMMENT     Sequence Label: pMLS338
FEATURES             Location/Qualifiers
     source          1..1006
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     27..43
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     28..44
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(159..179)
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     protein_bind    complement(369..402)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             complement(551..562)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 gggcgaattg ggtaccgggc cccccctcga ggtcgacggt atcgataagc ttgattacat
       61 taaagatttg tcgaacgtcg atgtcacact gtttgaagtg gccgaggtga tcttaacctt
      121 tgtatagttc atccatgcca tgtgtaatcc cagcagctgt tacaaactca agaaggacca
      181 tgtggtctct cttttcgttg ggatctttcg aaagggcaga ttgtgtggac aggtaatggt
      241 tgtctggtaa aaggacaggg ccatcgccaa ttggagtatt ttgttgataa tggtctgcta
      301 gttgaacgct tccatcttca atgttgtgtc taattttgaa gttctgaaaa tttaaatcag
      361 attagttaat aacttcgtat agcatacatt atacgaagtt atctttgagc caatttatcc
      421 aagtccttgt aaaaagtatt cgaaaattgt taacggataa aatgtttatt ataatatcaa
      481 aaacaatttg tcagttgacc actttttgat atatttgaca gaaaccggga tgaattggct
      541 caaagttagg gcgcccttct attacagggt ttctgataac aaaccggtta ttaactccca
      601 acaagggatg atttcaattc atcatgctca aattgaccca aattaagtta catgacaaat
      661 tcatcgccct tttcaactct tcttggctca tcatctgtta ttctgttcct attctgtctg
      721 caccctatac ccctttgcat actctctcgt cattcctttc ttggatagtg tcttcttctc
      781 cccagctctg ctacttctat gacttgcgcc cgctgctttt ccgctcgttc ctctcctctc
      841 tgacgtcttc ttctgcttct tttctccttc ctttttccct tctcaatctt cctctttttc
      901 ccatttctgt caatcattcg aagaagaaga agaagaagac cctcattcat ttcatttttt
      961 tttctgtcgg tgtgtgctgc tcggttaaga gtgagctctc tattcc
//
