LOCUS       sequence-139519-        1051 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  SapTrap Single plasmid targeting vector for tagging Unc-32 with GFP 
            by CRISPR/Cas9.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1051)
  AUTHORS   Schwartz ML, Jorgensen EM
  TITLE     SapTrap, a Toolkit for High-Throughput CRISPR/Cas9 Gene Modification
            in Caenorhabditis elegans.
  JOURNAL   Genetics. 2016 Feb 2. pii: genetics.115.184275.
  PUBMED    26837755
REFERENCE   2  (bases 1 to 1051)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genetics. 
            2016 Feb 2. pii: genetics.115.184275."
COMMENT     Sequence Label: pMLS338
FEATURES             Location/Qualifiers
     source          1..1051
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(184..212)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     protein_bind    771..804
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 cctcactaaa gggaacaaaa gctggagctc caccgcggtg gcggccaatt cgattgggct
       61 ccattctcat tcgaaaaaat ccttgctgaa gaacgtgaag ctgaggagaa tctcgcgggc
      121 agcggtggca gtggaggtac cggcggaagc ggtatgagca aaggagaaga acttttcact
      181 ggagttgtcc caattcttgt tgaattagat ggtgatgtta atgggcacaa attttctgtc
      241 agtggagagg gtgaaggtga tgcaacatac ggaaaactta cccttaaatt tatttgcact
      301 actggaaaac tacctgttcc atgggtaagt ttaaacatat atatactaac taaccctgat
      361 tatttaaatt ttcagccaac acttgtcact actttctgtt atggtgttca atgcttctcg
      421 agatacccag atcatatgaa acggcatgac tttttcaaga gtgccatgcc cgaaggttat
      481 gtacaggaaa gaactatatt tttcaaagat gacgggaact acaagacacg taagtttaaa
      541 cagttcggta ctaactaacc atacatattt aaattttcag gtgctgaagt caagtttgaa
      601 ggtgataccc ttgttaatag aatcgagtta aaaggtattg attttaaaga agatggaaac
      661 attcttggac acaaattgga atacaactat aactcacaca atgtatacat catggcagac
      721 aaacaaaaga atggaatcaa agttgtaagt ttaaacatga ttttactaac ataacttcgt
      781 ataatgtatg ctatacgaag ttatttctag acattctcta atgaaaaaat ctttcagttg
      841 aaattgaaaa tgagttaaag ttggagtttt tattgaaaac agatttccgt gtgattagtg
      901 tttttagcga gtgtgacagg acagcgaaaa aatatagaaa caagggggga actgaaaagc
      961 ttaggaatgc attgaacatg agaaggggaa ggggaaggaa caaactagac aggaattatt
     1021 ggaatttaat cacatttgga gttttttttc t
//
