LOCUS       sequence-139520-         988 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Photoconvertible eos in a middle entry clone.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 988)
  AUTHORS   Prendergast A, Linbo TH, Swarts T, Ungos JM, McGraw HF, Krispin S, 
            Weinstein BM, Raible DW
  TITLE     The metalloproteinase inhibitor Reck is essential for zebrafish DRG 
            development.
  JOURNAL   Development. 2012 Mar;139(6):1141-52. doi: 10.1242/dev.072439. Epub 
            2012 Feb 1.
  PUBMED    22296847
REFERENCE   2  (bases 1 to 988)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1242/dev.072439"; journalName: "Development"; date: "2012-03"; 
            volume: "139"; issue: "6"; pages: "1141-52"
COMMENT     Sequence Label: pME eos
FEATURES             Location/Qualifiers
     source          1..988
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     43..62
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     primer_bind     164..180
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     164..180
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     185..204
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        185..203
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    221..320
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
ORIGIN
        1 catgttggaa tttaatcgcg gcctcgacgt ttcccgttga atatggctca taacacccct
       61 tgtattactg tttatgtaag cagacagttt tattgttcat gatgatatat ttttatcttg
      121 tgcaatgtaa catcagagat tttgagacac gggccagagc tgccaggaaa cagctatgac
      181 catgtaatac gactcactat aggggatatc agctggatgg caaataatga ttttattttg
      241 actgatagtg acctgttcgt tgcaacaaat tgataagcaa tgctttctta taatgccaac
      301 tttgtacaag aaagctgggt ctatcgtctg gcattgtcag gcaatccaga atgagcaaca
      361 gcatgctcat acagcttaac cttgttgtaa tctttgtcat ggcttaaaat ctcaatgcag
      421 tggtccacaa agtggtagcc tggtaacttg acacccttct ccttagcttt gtaagtagtt
      481 ctgaagtcac atcggtaatg ggcatttcct tcaagcaaca aagccatggt aatatcaccc
      541 gtcagcactc catcacgcac atacattttc tcagtggagg gctcccattt cagcgtcttc
      601 ttctgcataa ctggaccatt ggcgggaaag tttacaccgt gaaatcgaac tttattatag
      661 aaagtgtccc cttccattgt tatgtcgttt ctggcaatgc aaatgccccc gtcttcgaaa
      721 gtcaagcttc gttcccacga atacccctta ggaaacgact gcttaaaata gtcttgtatg
      781 tggtctggat attcggcgaa taccctgttg ccgtaatgga atgcagtggt caggatatca
      841 aaggcaaaag gcagaggtcc gccctctttg acttcaagat ccatactctg ttttccctca
      901 aaaggcttgc ctgtaccatc tccgtcgatc acaaagtggt gcccgtttac gttgccttcc
      961 atacggaggt tgatcttcat gtctggct
//
