LOCUS       sequence-139522-        1047 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  SapTrap GFP + Cbr-unc-119 donor plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1047)
  AUTHORS   Schwartz ML, Jorgensen EM
  TITLE     SapTrap, a Toolkit for High-Throughput CRISPR/Cas9 Gene Modification
            in Caenorhabditis elegans.
  JOURNAL   Genetics. 2016 Feb 2. pii: genetics.115.184275.
  PUBMED    26837755
REFERENCE   2  (bases 1 to 1047)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genetics. 
            2016 Feb 2. pii: genetics.115.184275."
COMMENT     Sequence Label: pMLS252
FEATURES             Location/Qualifiers
     source          1..1047
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    66..90
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(129..157)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     protein_bind    716..749
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 ttttattttg actgatagtg acctgttcgt tgcaacaaat tgatgagcaa tgctttttta
       61 taatgccaac tttgtacaaa aaagcaggct gctcttcgat gagcaaagga gaagaacttt
      121 tcactggagt tgtcccaatt cttgttgaat tagatggtga tgttaatggg cacaaatttt
      181 ctgtcagtgg agagggtgaa ggtgatgcaa catacggaaa acttaccctt aaatttattt
      241 gcactactgg aaaactacct gttccatggg taagtttaaa catatatata ctaactaacc
      301 ctgattattt aaattttcag ccaacacttg tcactacttt ctgttatggt gttcaatgct
      361 tctcgagata cccagatcat atgaaacggc atgacttttt caagagtgcc atgcccgaag
      421 gttatgtaca ggaaagaact atatttttca aagatgacgg gaactacaag acacgtaagt
      481 ttaaacagtt cggtactaac taaccataca tatttaaatt ttcaggtgct gaagtcaagt
      541 ttgaaggtga tacccttgtt aatagaatcg agttaaaagg tattgatttt aaagaagatg
      601 gaaacattct tggacacaaa ttggaataca actataactc acacaatgta tacatcatgg
      661 cagacaaaca aaagaatgga atcaaagttg taagtttaaa catgatttta ctaacataac
      721 ttcgtataat gtatgctata cgaagttatt tctagacatt ctctaatgaa aaaatctttc
      781 agttgaaatt gaaaatgagt taaagttgga gtttttattg aaaacagatt tccgtgtgat
      841 tagtgttttt agcgagtgtg acaggacagc gaaaaaatat agaaacaagg ggggaactga
      901 aaagcttagg aatgcattga acatgagaag gggaagggga aggaacaaac tagacaggaa
      961 ttattggaat ttaatcacat ttggagtttt ttttctattc gacagaataa ttatccagaa
     1021 catttttgta ttaaatattt atgcatc
//
