LOCUS       sequence-139523-         976 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  SapTrap GFP + Cbr-unc-119 donor plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 976)
  AUTHORS   Schwartz ML, Jorgensen EM
  TITLE     SapTrap, a Toolkit for High-Throughput CRISPR/Cas9 Gene Modification
            in Caenorhabditis elegans.
  JOURNAL   Genetics. 2016 Feb 2. pii: genetics.115.184275.
  PUBMED    26837755
REFERENCE   2  (bases 1 to 976)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genetics. 
            2016 Feb 2. pii: genetics.115.184275."
COMMENT     Sequence Label: pMLS252
FEATURES             Location/Qualifiers
     source          1..976
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    11..110
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(162..182)
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     protein_bind    complement(372..405)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             complement(554..565)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 agctggatgg caaataatga ttttattttg actgatagtg acctgttcgt tgcaacaaat
       61 tgataagcaa tgctttctta taatgccaac tttgtacaag aaagctgggt gctcttcgac
      121 ctttgtatag ttcatccatg ccatgtgtaa tcccagcagc tgttacaaac tcaagaagga
      181 ccatgtggtc tctcttttcg ttgggatctt tcgaaagggc agattgtgtg gacaggtaat
      241 ggttgtctgg taaaaggaca gggccatcgc caattggagt attttgttga taatggtctg
      301 ctagttgaac gcttccatct tcaatgttgt gtctaatttt gaagttctga aaatttaaat
      361 cagattagtt aataacttcg tatagcatac attatacgaa gttatctttg agccaattta
      421 tccaagtcct tgtaaaaagt attcgaaaat tgttaacgga taaaatgttt attataatat
      481 caaaaacaat ttgtcagttg accacttttt gatatatttg acagaaaccg ggatgaattg
      541 gctcaaagtt agggcgccct tctattacag ggtttctgat aacaaaccgg ttattaactc
      601 ccaacaaggg atgatttcaa ttcatcatgc tcaaattgac ccaaattaag ttacatgaca
      661 aattcatcgc ccttttcaac tcttcttggc tcatcatctg ttattctgtt cctattctgt
      721 ctgcacccta tacccctttg catactctct cgtcattcct ttcttggata gtgtcttctt
      781 ctccccagct ctgctacttc tatgacttgc gcccgctgct tttccgctcg ttcctctcct
      841 ctctgacgtc ttcttctgct tcttttctcc ttcctttttc ccttctcaat cttcctcttt
      901 ttcccatttc tgtcaatcat tcgaagaaga agaagaagaa gaccctcatt catttcattt
      961 ttttttttcc tgtccg
//
