LOCUS       sequence-139525-        1071 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  SapTrap Halotag + Cbr-unc-119 donor plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1071)
  AUTHORS   Schwartz ML, Jorgensen EM
  TITLE     SapTrap, a Toolkit for High-Throughput CRISPR/Cas9 Gene Modification
            in Caenorhabditis elegans.
  JOURNAL   Genetics. 2016 Feb 2. pii: genetics.115.184275.
  PUBMED    26837755
REFERENCE   2  (bases 1 to 1071)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genetics. 
            2016 Feb 2. pii: genetics.115.184275."
COMMENT     Sequence Label: pMLS254
FEATURES             Location/Qualifiers
     source          1..1071
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    65..89
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    638..671
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 tttattttga ctgatagtga cctgttcgtt gcaacaaatt gatgagcaat gcttttttat
       61 aatgccaact ttgtacaaaa aagcaggctg ctcttcgatg atggctgaaa ttggtactgg
      121 ctttcctttt gatccccact acgttgaggt tcttggagag cgcatgcact acgttgatgt
      181 tggaccacgc gatggaaccc cagtcctttt ccttcacgga aacccaactt cttcttacgt
      241 ttggcgcaac atcatcccac atgttgctcc aacccaccgc tgcatcgctc cagatcttat
      301 cggaatggga aagtctgata agccagatct tggatacttc ttcgatgatc acgtccgttt
      361 catggatgct ttcatcgagg cccttggact tgaggaggtt gtccttgtta tccacgattg
      421 gggatccgct cttggattcc actgggctaa gcgcaaccca gagcgcgtta agggaatcgc
      481 tttcatggaa ttcatccgcc caatcccaac ttgggatgaa tggccagagt tcgctcgcga
      541 gaccttccaa gctttccgca ccaccgatgt tggacgtaag cttatcatcg atcaaaacgt
      601 tttcatcgaa ggtaagttta aacatgattt tactaacata acttcgtata atgtatgcta
      661 tacgaagtta tttctagaca ttctctaatg aaaaaatctt tcagttgaaa ttgaaaatga
      721 gttaaagttg gagtttttat tgaaaacaga tttccgtgtg attagtgttt ttagcgagtg
      781 tgacaggaca gcgaaaaaat atagaaacaa ggggggaact gaaaagctta ggaatgcatt
      841 gaacatgaga aggggaaggg gaaggaacaa actagacagg aattattgga atttaatcac
      901 atttggagtt ttttttctat tcgacagaat aattatccag aacatttttg tattaaatat
      961 ttatgcatca tatgagtagt cggctttgtt gtgcatgacg agtttgttat cgacgaaata
     1021 gaagctgtca gaacgagtct cgtttggatt gttgatcatg tcgtccactg a
//
