LOCUS       sequence-139526-        1034 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  SapTrap Halotag + Cbr-unc-119 donor plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1034)
  AUTHORS   Schwartz ML, Jorgensen EM
  TITLE     SapTrap, a Toolkit for High-Throughput CRISPR/Cas9 Gene Modification
            in Caenorhabditis elegans.
  JOURNAL   Genetics. 2016 Feb 2. pii: genetics.115.184275.
  PUBMED    26837755
REFERENCE   2  (bases 1 to 1034)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genetics. 
            2016 Feb 2. pii: genetics.115.184275."
COMMENT     Sequence Label: pMLS254
FEATURES             Location/Qualifiers
     source          1..1034
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    12..111
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(528..561)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             complement(710..721)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 cagctggatg gcaaataatg attttatttt gactgatagt gacctgttcg ttgcaacaaa
       61 ttgataagca atgctttctt ataatgccaa ctttgtacaa gaaagctggg tgctcttcga
      121 cctccggaga tctcaagggt agaaagccaa cgagcgatct cagatccgat aagatctggg
      181 ttatcctctt gaagaaggtt aagtcctggt ccgatatcga cagccttgca gtttggaaga
      241 gacttagcaa gacgggcagc ctcagctggt gggataagaa ctcctggagt tccccagaaa
      301 agaagctttg gaactggaga ttggtgaagc caatccatgt actcctcaac aagagcaacg
      361 atgttagctg gctctccagc gattggaagt tcgtttggga agcgccaaag tggctcgcga
      421 tcaactgggt taaggaatgg ctcgcggtag tgatccatct caacctcggt aagtggacga
      481 acaactccca ttggaagggt tcctgaaaat ttaaatcaga ttagttaata acttcgtata
      541 gcatacatta tacgaagtta tctttgagcc aatttatcca agtccttgta aaaagtattc
      601 gaaaattgtt aacggataaa atgtttatta taatatcaaa aacaatttgt cagttgacca
      661 ctttttgata tatttgacag aaaccgggat gaattggctc aaagttaggg cgcccttcta
      721 ttacagggtt tctgataaca aaccggttat taactcccaa caagggatga tttcaattca
      781 tcatgctcaa attgacccaa attaagttac atgacaaatt catcgccctt ttcaactctt
      841 cttggctcat catctgttat tctgttccta ttctgtctgc accctatacc cctttgcata
      901 ctctctcgtc attcctttct tggatagtgt cttcttctcc ccagctctgc tacttctatg
      961 acttgcgccc gctgcttttc cgctcgttcc tctcctctct gacgtcttct tctgcttctt
     1021 ttctccttcc tttt
//
