LOCUS       sequence-139528-        1045 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  SapTrap SNAPtag + Cbr-unc-119 donor plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1045)
  AUTHORS   Schwartz ML, Jorgensen EM
  TITLE     SapTrap, a Toolkit for High-Throughput CRISPR/Cas9 Gene Modification
            in Caenorhabditis elegans.
  JOURNAL   Genetics. 2016 Feb 2. pii: genetics.115.184275.
  PUBMED    26837755
REFERENCE   2  (bases 1 to 1045)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genetics. 
            2016 Feb 2. pii: genetics.115.184275."
COMMENT     Sequence Label: pMLS271
FEATURES             Location/Qualifiers
     source          1..1045
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    65..89
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    398..431
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 tttattttga ctgatagtga cctgttcgtt gcaacaaatt gatgagcaat gcttttttat
       61 aatgccaact ttgtacaaaa aagcaggctg ctcttcgatg gacaaggact gcgagatgaa
      121 gcgtaccacc ctcgactccc cactcggaaa gctcgagctc tccggatgcg agcaaggact
      181 ccaccgtatc atcttcctcg gaaagggaac ctccgccgcc gacgccgtcg aggtcccagc
      241 cccagccgcc gtcctcggag gaccagagcc actcatgcaa gccaccgcct ggctcaacgc
      301 ctacttccac caaccagagg ccatcgagga gttcccagtc ccagccctcc accacccagt
      361 cttccaacaa ggtaagttta aacatgattt tactaacata acttcgtata atgtatgcta
      421 tacgaagtta tttctagaca ttctctaatg aaaaaatctt tcagttgaaa ttgaaaatga
      481 gttaaagttg gagtttttat tgaaaacaga tttccgtgtg attagtgttt ttagcgagtg
      541 tgacaggaca gcgaaaaaat atagaaacaa ggggggaact gaaaagctta ggaatgcatt
      601 gaacatgaga aggggaaagg gaaggaacaa actagacagg aattattgga atttaatcac
      661 atttggagtt ttttttctat tcgacagaat aattatccag aacatttttg tattaaatat
      721 ttatgcatca tatgagtagt cggctttgtt gtgcatgacg agtttgttat cgacgaaata
      781 gaagctgtca gaacgagtct cgtttggatt gttgatcatg tcgtccactg aaaaagagat
      841 tagtcttttg aattgtactt tttagaataa tgactcactg agttgttgag agagttgagg
      901 gaactcatag atatgttcac agttgtttcg tgaattcgga atacagaatc cgaattcaaa
      961 gtcaaaacac ttcagaaggc gatctttgaa gaagtgacgt tcgatcattc ggaaatgatg
     1021 gattgggatg tctcctactt tgaat
//
