LOCUS       sequence-139529-         990 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  SapTrap SNAPtag + Cbr-unc-119 donor plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 990)
  AUTHORS   Schwartz ML, Jorgensen EM
  TITLE     SapTrap, a Toolkit for High-Throughput CRISPR/Cas9 Gene Modification
            in Caenorhabditis elegans.
  JOURNAL   Genetics. 2016 Feb 2. pii: genetics.115.184275.
  PUBMED    26837755
REFERENCE   2  (bases 1 to 990)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genetics. 
            2016 Feb 2. pii: genetics.115.184275."
COMMENT     Sequence Label: pMLS271
FEATURES             Location/Qualifiers
     source          1..990
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    8..107
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(413..446)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             complement(595..606)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 tggatggcaa ataatgattt tattttgact gatagtgacc tgttcgttgc aacaaattga
       61 taagcaatgc tttcttataa tgccaacttt gtacaagaaa gctgggtgct cttcgaccga
      121 gtcctggctt tccgagacgg tgtccctcgt gggcgaggag ccactccttg acggcgagtc
      181 ctccctcgta tcctccgacg tcgaggtctc cttggacgac acggtggcat gggatgagga
      241 ttgggactgg gtttccggag agggcggtct tgacggcggc ggtggcggct gggtttccgg
      301 cgagggcggc gaggtgggag taggagatga cctctccgaa cttgacgacc ttgaggagct
      361 tccagaggac ttgacgggtg aaggactctg aaaatttaaa tcagattagt taataacttc
      421 gtatagcata cattatacga agttatcttt gagccaattt atccaagtcc ttgtaaaaag
      481 tattcgaaaa ttgttaacgg ataaaatgtt tattataata tcaaaaacaa tttgtcagtt
      541 gaccactttt tgatatattt gacagaaacc gggatgaatt ggctcaaagt tagggcgccc
      601 ttctattaca gggtttctga taacaaaccg gttattaact cccaacaagg gatgatttca
      661 attcatcatg ctcaaattga cccaaattaa gttacatgac aaattcatcg cccttttcaa
      721 ctcttcttgg ctcatcatct gttattctgt tcctattctg tctgcaccct ataccccttt
      781 gcatactctc tcgtcattcc tttcttggat agtgtcttct tctccccagc tctgctactt
      841 ctatgacttg cgcccgctgc ttttccgctc gttcctctcc tctctgacgt cttcttctgc
      901 ttcttttctc cttccttttt cccttctcaa tcttcctctt tttcccattt ctgtcaatca
      961 ttcgaagaag aagaagaaga agaccctcat
//
