LOCUS       sequence-139540-        1021 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  contains cytoplasmic form of ovalbumin (OVA) cDNA in pBlueRIP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1021)
  AUTHORS   Kurts C, Heath WR, Carbone FR, Allison J, Miller JF, Kosaka H
  TITLE     Constitutive class I-restricted exogenous presentation of self 
            antigens in vivo.
  JOURNAL   J Exp Med. 1996 Sep 1;184(3):923-30.
  PUBMED    9064352
REFERENCE   2  (bases 1 to 1021)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Exp 
            Med."; date: "1996-09-1"; volume: "184(3)"; pages: "923-30"
COMMENT     Sequence Label: pBlueRIP.cOVA
FEATURES             Location/Qualifiers
     source          1..1021
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     rep_origin      complement(53..508)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(190..211)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     402..421
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     635..657
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     649..666
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     650..666
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     673..692
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        673..691
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     724..740
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     724..740
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     polyA_signal    737..871
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(761..780)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     815..834
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
ORIGIN
        1 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctaaattgta
       61 agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc attttttaac
      121 caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga gatagggttg
      181 agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc caacgtcaaa
      241 gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc ctaatcaagt
      301 tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag cccccgattt
      361 agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga
      421 gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac cacacccgcc
      481 gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg caactgttgg
      541 gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct
      601 gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg taaaacgacg
      661 gccagtgaat tgtaatacga ctcactatag ggcgaattgg agctccaccg cggtggcggc
      721 cgctctagaa ctagtggatc cagacatgat aagatacatt gatgagtttg gacaaaccac
      781 aactagaatg cagtgaaaaa aatgctttat ttgtgaaatt tgtgatgcta ttgctttatt
      841 tgtaaccatt ataagctgca ataaacaagt taacaacaac aattgcattc attttatgtt
      901 tcaggttcag ggggaggtgt gggaggtttt ttaaagcaag taaaacctct acaaatgtgg
      961 tatggctgat tatgatcatg aacagactgt gaggactgag gggcctgaaa tgagccttgg
     1021 g
//
