LOCUS       sequence-139542-         972 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Hairpin targeting MEK1 and MEK2 (murine)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 972)
  AUTHORS   Jensen KJ, Moyer CB, Janes KA
  TITLE     Network Architecture Predisposes an Enzyme to Either Pharmacologic 
            or Genetic Targeting.
  JOURNAL   Cell Syst. 2016 Feb 24;2(2):112-121.
  PUBMED    26942229
REFERENCE   2  (bases 1 to 972)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Syst."; date: "2016-02-24"; volume: "2(2)"; pages: "112-121"
COMMENT     Sequence Label: shMEK1/2 v2 neo
FEATURES             Location/Qualifiers
     source          1..972
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        117..365
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     117..137
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     288..307
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_feature    462..579
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ccgaaggaat agaagaagaa ggtggagaga gagacagaga cagatccatt cgattagtga
       61 acggatctcg acggtatcga tcacgagact agcctcgagc ggccgccccc ttcaccgagg
      121 gcctatttcc catgattcct tcatatttgc atatacgata caaggctgtt agagagataa
      181 ttggaattaa tttgactgta aacacaaaga tattagtaca aaatacgtga cgtagaaagt
      241 aataatttct tgggtagttt gcagttttaa aattatgttt taaaatggac tatcatatgc
      301 ttaccgtaac ttgaaagtat ttcgatttct tggctttata tatcttgtgg aaaggacgaa
      361 acaccgggct gatccacctg gagatcaact gcagttgatc tccaggtgga tcagcttttt
      421 gaattctcga cctcgagaca aatggcagta ttcatccaca attttaaaag aaaagggggg
      481 attggggggt acagtgcagg ggaaagaata gtagacataa tagcaacaga catacaaact
      541 aaagaattac aaaaacaaat tacaaaaatt caaaattttc gggtttatta cagggacagc
      601 agagatccac tttggccncg gctcgagggg gttggggttg cgccttttcc aaggcagccc
      661 tgggtttgcg cagggacgcg gctgctctgg gcgtggttcc gggaaacgca gcggcgccga
      721 ccctgggtct cgcacattct tcacgtccgt tcgcagcgtc acccggatct tcgccgctac
      781 ccttgtgggc cccccggcga cgcttcctgc tccgccccta agtcgggaag gttccttgcg
      841 gttcgcggcg tgccggacgt gacaaacgga agccgcacgt ctcactagta ccctcgcaga
      901 cggacagcgc cagggagcaa tggcagcgcg ccgaccgcga tgggctgtgg ccaatagcgg
      961 ctgctcagca gg
//
