LOCUS       sequence-139549-        1025 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Saptrap tagRFP + Cbr-unc-119 donor plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1025)
  AUTHORS   Schwartz ML, Jorgensen EM
  TITLE     SapTrap, a Toolkit for High-Throughput CRISPR/Cas9 Gene Modification
            in Caenorhabditis elegans.
  JOURNAL   Genetics. 2016 Feb 2. pii: genetics.115.184275.
  PUBMED    26837755
REFERENCE   2  (bases 1 to 1025)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genetics. 
            2016 Feb 2. pii: genetics.115.184275."
COMMENT     Sequence Label: pMLS286
FEATURES             Location/Qualifiers
     source          1..1025
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     27..43
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     28..44
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    629..662
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 gggcgaattg ggtaccgggc cccccctcga ggtcgacggt atcgataagc ttgatatcga
       61 attcctgcag cccgctcttc gatgatggtt gtgtcgaaag gcgaagaact gatcaaggaa
      121 aacatgcata tgaaactgta catggaagga accgtcaata accatcactt taaatgtact
      181 tccgaaggag aaggtaaacc atacgaaggc acccagacta tgcgcattaa agtcgttgaa
      241 ggaggtccac tgccgtttgc gttcgatatc ctggccacct catttatgta cggatctaga
      301 accttcatca accacactca gggtatccca gatttcttta aacaatcatt tccggaagga
      361 ttcacatggg aacgtgtgac cacttatgaa gatggcggag tcctgacagc aacgcaggat
      421 acgagcctgc aagatggttg cctgatttac aatgtcaaaa tccgcggtgt taattttcca
      481 agtaacggcc cggttatgca gaagaaaacc ctgggttggg aagcaaacac ggaaatgctg
      541 tatccagctg atggtggcct ggaaggcaga actgatatgg cactgaaact ggtgggaggt
      601 gggtaagttt aaacatgatt ttactaacat aacttcgtat aatgtatgct atacgaagtt
      661 atttctagac attctctaat gaaaaaatct ttcagttgaa attgaaaatg agttaaagtt
      721 ggagttttta ttgaaaacag atttccgtgt gattagtgtt tttagcgagt gtgacaggac
      781 agcgaaaaaa tatagaaaca aggggggaac tgaaaagctt aggaatgcat tgaacatgag
      841 aaggggaagg ggaaggaaca aactagacag gaattattgg aatttaatca catttggagt
      901 tttttttcta ttcgacagaa taattatcca gaacattttt gtattaaata tttatgcatc
      961 atatgagtag tcggctttgt tgtgcatgac gagtttgtta tcgacgaaat agaagctgtc
     1021 agaac
//
