LOCUS       sequence-139550-         990 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Saptrap tagRFP + Cbr-unc-119 donor plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 990)
  AUTHORS   Schwartz ML, Jorgensen EM
  TITLE     SapTrap, a Toolkit for High-Throughput CRISPR/Cas9 Gene Modification
            in Caenorhabditis elegans.
  JOURNAL   Genetics. 2016 Feb 2. pii: genetics.115.184275.
  PUBMED    26837755
REFERENCE   2  (bases 1 to 990)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genetics. 
            2016 Feb 2. pii: genetics.115.184275."
COMMENT     Sequence Label: pMLS286
FEATURES             Location/Qualifiers
     source          1..990
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     49..65
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     49..65
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     protein_bind    complement(323..356)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             complement(505..516)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 cctcactaaa gggaacaaaa gctggagctc caccgcggtg gcggccgctc tagaactagt
       61 ggatcccccg ctcttcgacc tttgtacagt tcatccattc cattcagttt gtggcccagt
      121 ttgctcggca gatcgcagta acgagcgact gccacttcat gttgttccac ataggtttct
      181 ttatcggctt ctttgatgcg ttccagacgg tgatccacgt aataaactcc cggcattttc
      241 aggtttttcg ctggtttttt agaacgatac gttgtcttga agttacagat cagatggctg
      301 aaaatttaaa tcagattagt taataacttc gtatagcata cattatacga agttatcttt
      361 gagccaattt atccaagtcc ttgtaaaaag tattcgaaaa ttgttaacgg ataaaatgtt
      421 tattataata tcaaaaacaa tttgtcagtt gaccactttt tgatatattt gacagaaacc
      481 gggatgaatt ggctcaaagt tagggcgccc ttctattaca gggtttctga taacaaaccg
      541 gttattaact cccaacaagg gatgatttca attcatcatg ctcaaattga cccaaattaa
      601 gttacatgac aaattcatcg cccttttcaa ctcttcttgg ctcatcatct gttattctgt
      661 tcctattctg tctgcaccct ataccccttt gcatactctc tcgtcattcc tttcttggat
      721 agtgtcttct tctccccagc tctgctactt ctatgacttg cgcccgctgc ttttccgctc
      781 gttcctctcc tctctgacgt cttcttctgc ttcttttctc cttccttttt cccttctcaa
      841 tcttcctctt tttcccattt ctgtcaatca ttcgaagaag aagaagaaga agaccctcat
      901 tcatttcatt tttttttctg tcggtgtgtg ctgctcggtt aagagtgagc tctctattcc
      961 acgtcttctt ctttttcttc ttcgattcga
//
