LOCUS       sequence-139551-        1025 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  SapTrap mCherry + Cbr-unc-119 donor plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1025)
  AUTHORS   Schwartz ML, Jorgensen EM
  TITLE     SapTrap, a Toolkit for High-Throughput CRISPR/Cas9 Gene Modification
            in Caenorhabditis elegans.
  JOURNAL   Genetics. 2016 Feb 2. pii: genetics.115.184275.
  PUBMED    26837755
REFERENCE   2  (bases 1 to 1025)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genetics. 
            2016 Feb 2. pii: genetics.115.184275."
COMMENT     Sequence Label: pMLS291
FEATURES             Location/Qualifiers
     source          1..1025
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    61..85
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    765..798
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 ttttgactga tagtgacctg ttcgttgcaa caaattgatg agcaatgctt ttttataatg
       61 ccaactttgt acaaaaaagc aggctgctct tcgatggtct caaagggtga agaagataac
      121 atggcaatta ttaaagagtt tatgcgtttc aaggtgcata tggagggatc tgtcaatggg
      181 catgagtttg aaattgaagg tgaaggagaa ggccgaccat atgagggaac acaaaccgca
      241 aaactaaagg taagtttaaa catatatata ctaactaacc ctgattattt aaattttcag
      301 gtaactaaag gcggaccatt accattcgcc tgggacatcc tctctccaca gttcatgtat
      361 ggaagtaaag cttatgttaa acatccggca gatataccag attatttgaa actttcattc
      421 ccggagggtt ttaagtggga acgcgtaatg aattttgaag acggaggagt tgttacagtg
      481 acgcaagact caaggtaagt ttaaacagtt cggtactaac taaccataca tatttaaatt
      541 ttcagcctcc aagatggaga atttatttat aaagtcaaac ttcgaggaac gaatttcccc
      601 tcggatggac ctgttatgca gaagaagact atgggatggg aagcttcaag tgaaagaatg
      661 taccctgaag acggtgctct taagggagag attaaacaac gtcttaaatt gaaagatgga
      721 ggacattacg atgctgaggt aagtttaaac atgattttac taacataact tcgtataatg
      781 tatgctatac gaagttattt ctagacattc tctaatgaaa aaatctttca gttgaaattg
      841 aaaatgagtt aaagttggag tttttattga aaacagattt ccgtgtgatt agtgttttta
      901 gcgagtgtga caggacagcg aaaaaatata gaaacaaggg gggaactgaa aagcttagga
      961 atgcattgaa catgagaagg ggaaggggaa ggaacaaact agacaggaat tattggaatt
     1021 taatc
//
