LOCUS       sequence-139552-         953 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  SapTrap mCherry + Cbr-unc-119 donor plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 953)
  AUTHORS   Schwartz ML, Jorgensen EM
  TITLE     SapTrap, a Toolkit for High-Throughput CRISPR/Cas9 Gene Modification
            in Caenorhabditis elegans.
  JOURNAL   Genetics. 2016 Feb 2. pii: genetics.115.184275.
  PUBMED    26837755
REFERENCE   2  (bases 1 to 953)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genetics. 
            2016 Feb 2. pii: genetics.115.184275."
COMMENT     Sequence Label: pMLS291
FEATURES             Location/Qualifiers
     source          1..953
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    12..111
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(313..346)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             complement(495..506)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 cagctggatg gcaaataatg attttatttt gactgatagt gacctgttcg ttgcaacaaa
       61 ttgataagca atgctttctt ataatgccaa ctttgtacaa gaaagctggg tgctcttcga
      121 cccttataca attcatccat gccacctgtc gagtgccgcc cttcagctct ttcatattgc
      181 tcaacgatag tgtaatcctc gttgtgggag gtgatatcca gtttaatatt aacattgtac
      241 gctcctggca gctgaactgg ttttttggct ttgtaagttg tcttcacctg aaaatttaaa
      301 tcagattagt taataacttc gtatagcata cattatacga agttatcttt gagccaattt
      361 atccaagtcc ttgtaaaaag tattcgaaaa ttgttaacgg ataaaatgtt tattataata
      421 tcaaaaacaa tttgtcagtt gaccactttt tgatatattt gacagaaacc gggatgaatt
      481 ggctcaaagt tagggcgccc ttctattaca gggtttctga taacaaaccg gttattaact
      541 cccaacaagg gatgatttca attcatcatg ctcaaattga cccaaattaa gttacatgac
      601 aaattcatcg cccttttcaa ctcttcttgg ctcatcatct gttattctgt tcctattctg
      661 tctgcaccct ataccccttt gcatactctc tcgtcattcc tttcttggat agtgtcttct
      721 tctccccagc tctgctactt ctatgacttg cgcccgctgc ttttccgctc gttcctctcc
      781 tctctgacgt cttcttctgc ttcttttctc cttccttttt cccttctcaa tcttcctctt
      841 tttcccattt ctgtcaatca ttcgaagaag aagaagaaga agaccctcat tcatttcatt
      901 tttttttctg tcggtgtgtg ctgctcggtt aagagtgagc tctctattcc acg
//
