LOCUS       sequence-139554-         964 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  SapTrap 2xNLS-mCherry + Cbr-unc-119 donor plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 964)
  AUTHORS   Schwartz ML, Jorgensen EM
  TITLE     SapTrap, a Toolkit for High-Throughput CRISPR/Cas9 Gene Modification
            in Caenorhabditis elegans.
  JOURNAL   Genetics. 2016 Feb 2. pii: genetics.115.184275.
  PUBMED    26837755
REFERENCE   2  (bases 1 to 964)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genetics. 
            2016 Feb 2. pii: genetics.115.184275."
COMMENT     Sequence Label: pMLS292
FEATURES             Location/Qualifiers
     source          1..964
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    61..85
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             103..123
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     protein_bind    810..843
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 ttttgactga tagtgacctg ttcgttgcaa caaattgatg agcaatgctt ttttataatg
       61 ccaactttgt acaaaaaagc aggctgctct tcgatgactg ctccaaagaa gaagcgtaag
      121 gtaccggtag aaaaagggat ggtctcaaag ggtgaagaag ataacatggc aattattaaa
      181 gagtttatgc gtttcaaggt gcatatggag ggatctgtca atgggcatga gtttgaaatt
      241 gaaggtgaag gagaaggccg accatatgag ggaacacaaa ccgcaaaact aaaggtaagt
      301 ttaaacatat atatactaac taaccctgat tatttaaatt ttcaggtaac taaaggcgga
      361 ccattaccat tcgcctggga catcctctct ccacagttca tgtatggaag taaagcttat
      421 gttaaacatc cggcagatat accagattat ttgaaacttt cattcccgga gggttttaag
      481 tgggaacgcg taatgaattt tgaagacgga ggagttgtta cagtgacgca agactcaagg
      541 taagtttaaa cagttcggta ctaactaacc atacatattt aaattttcag cctccaagat
      601 ggagaattta tttataaagt caaacttcga ggaacgaatt tcccctcgga tggacctgtt
      661 atgcagaaga agactatggg atgggaagct tcaagtgaaa gaatgtaccc tgaagacggt
      721 gctcttaagg gagagattaa acaacgtctt aaattgaaag atggaggaca ttacgatgct
      781 gaggtaagtt taaacatgat tttactaaca taacttcgta taatgtatgc tatacgaagt
      841 tatttctaga cattctctaa tgaaaaaatc tttcagttga aattgaaaat gagttaaagt
      901 tggagttttt attgaaaaca gatttccgtg tgattagtgt ttttagcgag tgtgacagga
      961 cagc
//
