LOCUS       sequence-139561-         460 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  SapTrap SL2::SV40-NLS C-terminal connector donor plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 460)
  AUTHORS   Schwartz ML, Jorgensen EM
  TITLE     SapTrap, a Toolkit for High-Throughput CRISPR/Cas9 Gene Modification
            in Caenorhabditis elegans.
  JOURNAL   Genetics. 2016 Feb 2. pii: genetics.115.184275.
  PUBMED    26837755
REFERENCE   2  (bases 1 to 460)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genetics. 
            2016 Feb 2. pii: genetics.115.184275."
COMMENT     Sequence Label: pMLS269
FEATURES             Location/Qualifiers
     source          1..460
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    12..111
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(136..156)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     protein_bind    complement(428..452)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 cagctggatg gcaaataatg attttatttt gactgatagt gacctgttcg ttgcaacaaa
       61 ttgataagca atgctttctt ataatgccaa ctttgtacaa gaaagctggg tagctcttcg
      121 cattttttct accggtacct tacgcttctt ctttggagca gtcattgatg cgttgaagca
      181 gtttccctga attaaaatta gaagttgaaa atacaataga aagaaaattg gaaggtatat
      241 aaactcgcaa aaacgaggaa atggcagatt caataagatg aataaatata caacctttat
      301 tgagaagaga ccatttatat acttgtaagc ttctaggaaa attttagata ctaaagagaa
      361 gcatagattt taagacaagc agttaactag gtgaaagtag gatgagacag cttacgccga
      421 agagcatagc ctgctttttt gtacaaagtt ggcattataa
//
