LOCUS       sequence-139562-         508 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  SapTrap SL2::SV40-NLS C-terminal connector donor plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 508)
  AUTHORS   Schwartz ML, Jorgensen EM
  TITLE     SapTrap, a Toolkit for High-Throughput CRISPR/Cas9 Gene Modification
            in Caenorhabditis elegans.
  JOURNAL   Genetics. 2016 Feb 2. pii: genetics.115.184275.
  PUBMED    26837755
REFERENCE   2  (bases 1 to 508)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genetics. 
            2016 Feb 2. pii: genetics.115.184275."
COMMENT     Sequence Label: pMLS269
FEATURES             Location/Qualifiers
     source          1..508
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    68..92
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             364..384
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     protein_bind    complement(409..508)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
ORIGIN
        1 gattttattt tgactgatag tgacctgttc gttgcaacaa attgatgagc aatgcttttt
       61 tataatgcca actttgtaca aaaaagcagg ctatgctctt cggcgtaagc tgtctcatcc
      121 tactttcacc tagttaactg cttgtcttaa aatctatgct tctctttagt atctaaaatt
      181 ttcctagaag cttacaagta tataaatggt ctcttctcaa taaaggttgt atatttattc
      241 atcttattga atctgccatt tcctcgtttt tgcgagttta tataccttcc aattttcttt
      301 ctattgtatt ttcaacttct aattttaatt cagggaaact gcttcaacgc atcaatgact
      361 gctccaaaga agaagcgtaa ggtaccggta gaaaaaatgc gaagagctac ccagctttct
      421 tgtacaaagt tggcattata agaaagcatt gcttatcaat ttgttgcaac gaacaggtca
      481 ctatcagtca aaataaaatc attatttg
//
