LOCUS       sequence-139563-         988 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  SapTrap syntron-embedded FLP-on C-terminal connector donor plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 988)
  AUTHORS   Schwartz ML, Jorgensen EM
  TITLE     SapTrap, a Toolkit for High-Throughput CRISPR/Cas9 Gene Modification
            in Caenorhabditis elegans.
  JOURNAL   Genetics. 2016 Feb 2. pii: genetics.115.184275.
  PUBMED    26837755
REFERENCE   2  (bases 1 to 988)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genetics. 
            2016 Feb 2. pii: genetics.115.184275."
COMMENT     Sequence Label: pMLS272
FEATURES             Location/Qualifiers
     source          1..988
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    67..91
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    129..162
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     protein_bind    601..634
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     protein_bind    complement(707..806)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(823..842)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(824..842)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(847..863)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(847..863)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(965..984)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
ORIGIN
        1 attttatttt gactgatagt gacctgttcg ttgcaacaaa ttgatgagca atgctttttt
       61 ataatgccaa ctttgtacaa aaaagcaggc tgctcttcgg cggtaagttt aaacatgatt
      121 ttactaacga agttcctatt ctctagaaag tataggaact tctaactaat ctgatttaaa
      181 ttttcagggt taaattttca aattttaaat actgaatatt tgtttttttt cctattattt
      241 atttattctc tttgtgtttt ttttcttgct ttctaaaaaa ttaattcaat ccaaatctaa
      301 acattttttt ttctctttcc gtctcccaat tcgtattccg ctcctctcat ctgaacacaa
      361 tgtgcaagtt tatttatctt ctcgctttca tttcattagg acgtgggggg aattggtgga
      421 agggggaaac acacaaaagg atgatggaaa tgaaataagg acacacaata tgcaacaaca
      481 ttcaattcag aaatatggag gaaggtttaa aagaaaacat aaaaatatat agaggaggaa
      541 ggaaaactag taaaaaataa gcaaagaaat taggcgaacg atgagaattg tcctcgcttg
      601 gaagttccta ttctctagaa agtataggaa cttctaacta atctgattta aattttcagg
      661 gtagcggtgg cagtggaggt accggcggaa gcggtatggg aagagcaccc agctttcttg
      721 tacaaagttg gcattataag aaagcattgc ttatcaattt gttgcaacga acaggtcact
      781 atcagtcaaa ataaaatcat tatttgccat ccagctgata tcccctatag tgagtcgtat
      841 tacatggtca tagctgtttc ctggcagctc tggcccgtgt ctcaaaatct ctgatgttac
      901 attgcacaag ataaaataat atcatcatga acaataaaac tgtctgctta cataaacagt
      961 aatacaaggg gtgttatgag ccatattc
//
