LOCUS       sequence-139564-         848 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  SapTrap syntron-embedded FLP-on C-terminal connector donor plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 848)
  AUTHORS   Schwartz ML, Jorgensen EM
  TITLE     SapTrap, a Toolkit for High-Throughput CRISPR/Cas9 Gene Modification
            in Caenorhabditis elegans.
  JOURNAL   Genetics. 2016 Feb 2. pii: genetics.115.184275.
  PUBMED    26837755
REFERENCE   2  (bases 1 to 848)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genetics. 
            2016 Feb 2. pii: genetics.115.184275."
COMMENT     Sequence Label: pMLS272
FEATURES             Location/Qualifiers
     source          1..848
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    8..107
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(180..213)
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     protein_bind    complement(652..685)
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     protein_bind    complement(723..822)
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
ORIGIN
        1 tggatggcaa ataatgattt tattttgact gatagtgacc tgttcgttgc aacaaattga
       61 taagcaatgc tttcttataa tgccaacttt gtacaagaaa gctgggtgct cttcccatac
      121 cgcttccgcc ggtacctcca ctgccaccgc taccctgaaa atttaaatca gattagttag
      181 aagttcctat actttctaga gaataggaac ttccaagcga ggacaattct catcgttcgc
      241 ctaatttctt tgcttatttt ttactagttt tccttcctcc tctatatatt tttatgtttt
      301 cttttaaacc ttcctccata tttctgaatt gaatgttgtt gcatattgtg tgtccttatt
      361 tcatttccat catccttttg tgtgtttccc ccttccacca attcccccca cgtcctaatg
      421 aaatgaaagc gagaagataa ataaacttgc acattgtgtt cagatgagag gagcggaata
      481 cgaattggga gacggaaaga gaaaaaaaaa tgtttagatt tggattgaat taatttttta
      541 gaaagcaaga aaaaaaacac aaagagaata aataaataat aggaaaaaaa acaaatattc
      601 agtatttaaa atttgaaaat ttaaccctga aaatttaaat cagattagtt agaagttcct
      661 atactttcta gagaatagga acttcgttag taaaatcatg tttaaactta ccgccgaaga
      721 gcagcctgct tttttgtaca aagttggcat tataaaaaag cattgctcat caatttgttg
      781 caacgaacag gtcactatca gtcaaaataa aatcattatt tggggcccga gcttaagact
      841 ggccgtcg
//
