LOCUS       sequence-139566-       15504 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses tdTomato for monosynaptic circuit mapping.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 15504)
  AUTHORS   Reardon TR, Murray AJ, Turi GF, Wirblich C, Croce KR, Schnell MJ, 
            Jessell TM, Losonczy A
  TITLE     Rabies Virus CVS-N2c Strain Enhances Retrograde Synaptic Transfer 
            and Neuronal Viability.
  JOURNAL   Neuron. 2016 Jan 19. pii: S0896-6273(16)00005-2. doi: 
            10.1016/j.neuron.2016.01.004.
  PUBMED    26804990
REFERENCE   2  (bases 1 to 15504)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Neuron. 
            2016 Jan 19. pii: S0896-6273(16)00005-2. doi: 
            10.1016/j.neuron.2016.01.004."
COMMENT     Sequence Label: RabV CVS-N2c(deltaG)-tdTomato
FEATURES             Location/Qualifiers
     source          1..15504
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      3319..3328
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3325..4755
                     /codon_start=1
                     /product="tandem dimeric (pseudo-monomeric) derivative of 
                     DsRed (Shaner et al., 2004)"
                     /label=tdTomato
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLGHGTGSTGSGSSGTASSEDNNMAVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPY
                     EGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVM
                     NFEDGGLVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGV
                     LKGEIHQALKLKDGGRYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYE
                     RSEGRHHLFLYGMDELYK"
     CDS             6933..6944
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             complement(8736..8747)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(11944..11962)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      11958..12005
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(12033..12049)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(12033..12049)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(12046..12068)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    12057..12073
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(12081..12087,12088..12105,12106..12111))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12126..12147
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(12264..12281)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(12435..13023)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(12515..12534)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(13194..13985,13986..14054))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     13817..13836
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(14055..14159)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      14186..14641
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(14273..14292)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     14483..14504
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     14767..14789
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     14781..14798
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     14782..14798
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     enhancer        14868..15171
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        15172..15375
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     15326..15346
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     15420..15439
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        15420..15438
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 acgcttaaca acaaaaccag agaagaaaaa gacagcgtca attgcaaagc aaaaatgtaa
       61 cacccctaca atggatgccg acaagattgt gttcaaagtc aataatcagg tggtctcttt
      121 gaagcctgag attatcgtgg atcaatatga gtacaagtac cctgccatca aggatttgaa
      181 aaagccttgt atcaccctag ggaaagcccc cgacttgaac aaagcataca aatcagtttt
      241 atcaggcatg aatgccgcca aacttgatcc ggatgatgta tgctcctact tggcagcagc
      301 aatgcagttc tttgagggga catgtccgga agactggacc agctatggaa tcctgattgc
      361 acgaaaagga gataggatca ccccaaactc tctagtggag ataaagcgta ctgatgtaga
      421 agggaattgg gctctgacag gaggcatgga attgacaagg gaccccactg tctctgaaca
      481 tgcatcttta gtcggtcttc tcctgagtct gtacaggttg agcaaaatat caggacagaa
      541 cactggtaac tataagacaa acattgcaga taggatagag cagattttcg agacagcacc
      601 ttttgttaag atcgtggaac accataccct aatgacaact cacaagatgt gtgctaattg
      661 gagtactata ccgaacttca gatttttggc cggaacctac gacatgtttt tctcacggat
      721 tgagcatcta tattcggcaa tcagagtggg cacagtcgtc accgcttatg aagactgctc
      781 aggactggta tcgtttacag ggttcataaa gcagatcaat ctcaccgcaa gggaagcaat
      841 actatatttc ttccacaaga actttgagga agagataaga agaatgttcg agccagggca
      901 agagacagct gttcctcact cttatttcat ccacttccgt tcactaggct tgagtgggaa
      961 gtctccttat tcatcgaatg ctgtcggtca tgtgttcaat ctcattcact ttgttggatg
     1021 ctacatgggt caagtcagat ctctaaatgc gacggttatt gctgcgtgtg cccctcatga
     1081 gatgtctgtt ctagggggct atttgggaga ggaattcttc ggaaaaggga catttgaaag
     1141 aaggttcttc agagacgaga aagaacttca agaatatgag gcggctgaac taacaaagtc
     1201 cgacgtggca ctggcagatg acggaaccgt caactctgat gacgaggact atttctctgg
     1261 tgaaaccaga agtccagaag ctgtctatac tcgaatcatg atgaatggag gtcgactgaa
     1321 gagatctcat atacggagat atgtctcagt cagttccaat catcaagccc gtccaaactc
     1381 attcgccgaa tttttaaaca agacgtattc gaatgactca taaggagttg attgacaggg
     1441 tgccagaaat ctatagattg tatatatcca tcatgaaaaa aactaacact cctcctttca
     1501 aaccatccca aatatgagca agatctttgt taatccgagt gcaatcagag ccggtctggc
     1561 cgatcttgag atggccgaag agactgttga tctgatcaac agaaacatag aagacaatca
     1621 ggctcatctc cagggagaac ccatagaagt ggacaacctc cctgaggaca tgaagcgact
     1681 tcacctggac gatgaaaaat cgtccaacct tggtgagatg gttagggtgg gagaaggcaa
     1741 gtatcgagag gactttcaga tggatgaggg agaggacccc aacctcctgt tccaatcgta
     1801 cctggataat gttggagtcc aaatagtcag acaaatgagg tcaggagaga gattcctcaa
     1861 gatatggtca cagaccgtag aggaaattgt atcctatgtc acggtcaact ttcctaaccc
     1921 tccaagaagg tcttcggagg ataaatcaac ccagactact ggcagagagc tcaagaagga
     1981 gacaacgtct gctttctctc agagagaaag ccaaccttcg aaagctagga tggtggctca
     2041 agttgcccct ggtcctccag cccttgaatg gtcagccacc aatgaagaag atgatctatc
     2101 agtagaggct gagatcgctc atcagattgc tgaaagcttt tccaagaagt acaagtttcc
     2161 ctcccgatct tcaggaatat tcttgtataa ttttgagcaa ctgaagatga accttgatga
     2221 catagttaaa gaggcaaaaa atgtaccggg cgtgacccgt ctggcccatg atggatccaa
     2281 aatccccctg agatgcgtac tgggatgggt cgctttggct aattccaaaa aattccaatt
     2341 actagtcgag gctgacaagc taagcaaaat catgcaagat gatttgaatc gctatacatc
     2401 ctgctaaccg agttttcgaa ctcagtccct ccagataatg aaaactgaga tgttatggag
     2461 tagacatgaa aaaaacaggc aacaccactg ataaaatgaa cgttctacgc aagatagtga
     2521 aaaaatgtag ggatgaggac actcaaaagc cctctcctgt gtcagcccct ccgtatgacg
     2581 atgacctgtg gcttccacct cctgaatatg tcccgctgaa agaactcaca agcaagaaga
     2641 acatgaggaa cttttgtgtc aacggggagg ttaaagcgtg tagcccaaat ggttactcat
     2701 tcaggatttt gcggcacatt ctgagatcat tcaacgagat atactctggg aatcatagga
     2761 tgattgggtt agtcaaaatt gttgttggac tagctttatc aggagctcca gtacctgagg
     2821 gcatgaactg ggtatacaaa ttgaggagaa cccttatatt ccagtgggct gattccaggg
     2881 gccctcttga aggggaggag ttagattact ctcaagagat cacttgggat gatgatactg
     2941 aattcgtcgg attgcaaata agagtgagcg caagacaatg tcatattcaa ggcaggatct
     3001 ggtgtatcaa cacgaactcg agggcatgtc aactatggtc tgacatgtct cttcagacac
     3061 aaaggtctga agaggacaaa gactcttctc tgcttctaga ataatcagat tatatcctgc
     3121 aagtgtatca cttgtttacc tctggaggag agagcataca ggcttgactc cgatccttgg
     3181 gagcaataga acaaaaaaac acacgttatg gtgccgttaa atcgctgcat tttatcaaag
     3241 tcaagttgat aacctttaca ttttgagcct cttggatgtg aaaaaaacta ttaacatccc
     3301 tcaaaagacc ccgggaaagc caccatggtg agcaagggcg aggaggtcat caaagagttc
     3361 atgcgcttca aggtgcgcat ggagggctcc atgaacggcc acgagttcga gatcgagggc
     3421 gagggcgagg gccgccccta cgagggcacc cagaccgcca agctgaaggt gaccaagggc
     3481 ggccccctgc ccttcgcctg ggacatcctg tccccccagt tcatgtacgg ctccaaggcg
     3541 tacgtgaagc accccgccga catccccgat tacaagaagc tgtccttccc cgagggcttc
     3601 aagtgggagc gcgtgatgaa cttcgaggac ggcggtctgg tgaccgtgac ccaggactcc
     3661 tccctgcagg acggcacgct gatctacaag gtgaagatgc gcggcaccaa cttccccccc
     3721 gacggccccg taatgcagaa gaagaccatg ggctgggagg cctccaccga gcgcctgtac
     3781 ccccgcgacg gcgtgctgaa gggcgagatc caccaggccc tgaagctgaa ggacggcggc
     3841 cactacctgg tggagttcaa gaccatctac atggccaaga agcccgtgca actgcccggc
     3901 tactactacg tggacaccaa gctggacatc acctcccaca acgaggacta caccatcgtg
     3961 gaacagtacg agcgctccga gggccgccac cacctgttcc tggggcatgg caccggcagc
     4021 accggcagcg gcagctccgg caccgcctcc tccgaggaca acaacatggc cgtcatcaaa
     4081 gagttcatgc gcttcaaggt gcgcatggag ggctccatga acggccacga gttcgagatc
     4141 gagggcgagg gcgagggccg cccctacgag ggcacccaga ccgccaagct gaaggtgacc
     4201 aagggcggcc ccctgccctt cgcctgggac atcctgtccc cccagttcat gtacggctcc
     4261 aaggcgtacg tgaagcaccc cgccgacatc cccgattaca agaagctgtc cttccccgag
     4321 ggcttcaagt gggagcgcgt gatgaacttc gaggacggcg gtctggtgac cgtgacccag
     4381 gactcctccc tgcaggacgg cacgctgatc tacaaggtga agatgcgcgg caccaacttc
     4441 ccccccgacg gccccgtaat gcagaagaag accatgggct gggaggcctc caccgagcgc
     4501 ctgtaccccc gcgacggcgt gctgaagggc gagatccacc aggccctgaa gctgaaggac
     4561 ggcggccgct acctggtgga gttcaagacc atctacatgg ccaagaagcc cgtgcaactg
     4621 cccggctact actacgtgga caccaagctg gacatcacct cccacaacga ggactacacc
     4681 atcgtggaac agtacgagcg ctccgagggc cgccaccacc tgttcctgta cggcatggac
     4741 gagctgtaca agtaataggc tagcggcgcc tagccggtca tccttttgac gattccagtc
     4801 ccgaggataa cctcctctcg ggattggggg gaatctttgg atccagtagt cctccttgaa
     4861 ctccgtccaa cagggcagat tcaagagtca taagactttc attaatcatt tcagttgatc
     4921 agacatggtc gtgtagattc tcttaatacg ggagatcttc tagcagtttc agtgaccaac
     4981 ggtgctttca ttctccagga actgatacca aaggttgtgg acaggccaag gggtacttcg
     5041 gatgactctg tgcttgggca cagaaagagg tcgtagtgtg ccccccgata gcggactcga
     5101 catgaatcaa ctaagaaagg caatctgcct cccatgatgg acataagcaa tagttcacaa
     5161 ccatcttgca tctcagtgaa gtgtacataa ctattgaggg ctgggtcatc taagcatttc
     5221 agtcgagaaa aaaactgtag accaaaagaa caactagcaa cacttctcat ccagagaccc
     5281 atatcaagat gctagatccg ggagaggttt atgatgaccc tattgatcca attgagtcag
     5341 aggctgaacc cagaggaacc cccactgtcc ccaacatctt gaggaactcc gactacaatc
     5401 tcaattctcc tttgatagag gatcctgcca aactaatgtt agaatggttg aagacaggga
     5461 acagacctta tcggatgact ttgacagaca attgctccag gtcttacaaa gttttgaaag
     5521 attatttcaa gaaagtagat ttgggttctc tcaaagtggg cggaactgct gcacagtcaa
     5581 tggtttctct ctggttgtgt ggtgcccact ctgaatcaaa caggagccgg agatgtataa
     5641 ccgacttggc ccatttctat tccaagtcat cccccataga gaagctattg aattgtacgc
     5701 taggaaacag aggcctgaga atcccaccag agggggtgtt aaattgcctc gagagggtca
     5761 attatgacaa ggcatttggg aggtatctgg ccaacacgta ttcctcttac ttgtttttcc
     5821 atgtaatcac cttatacatg aatgccttag actgggaaga ggaaaagacc atcctggcat
     5881 tatggaaaga tataacctca gtggataccg agaaggactt ggtcaaattc aaagatcaaa
     5941 tatggggact gttgattgtg acaaaggact ttgtttactc tcagagttca aactgtcttt
     6001 ttgacagaaa ctacacactg atgctaaagg atcttttctt gtctcgattc aactccttaa
     6061 tgattctgct ttctccccct gagccccgat actcagatga cttaatatct cagctgtgcc
     6121 agctatacat cgctggggat caagtcttgt ccatgtgtgg gaactccggc tatgaagtca
     6181 tcaaaatatt ggagccatat gtcgtgaaca gtttggtcca gagggcagag aagtttaggc
     6241 ctctcatcca ccccttggga gactttccta tgttcataaa agacaaggtg aatcaacttg
     6301 aagggacttt tggtcccagt gcaaagaggt tttttagggt tttagatcaa ttcgacaaca
     6361 tacatgactt agtatttgtg tatggctgtt acagacattg ggggcacccc tatatagatt
     6421 atcggaaggg tctgtcgaaa ctatatgatc aagttcacat taagaaagta atagataagt
     6481 cctaccagga gtgtttagca agtgacttgg ccagaaggat cctcagatgg ggatttgaca
     6541 agtactccaa atggtatcta gattcgagat tccttgcccg agaccacccc ttgactcctt
     6601 atgtcaagac ccaaacatgg ccacccaaac atatagtaga cttggtgggg gacacatggc
     6661 ataagctccc gatcacgcag atctttgaaa ttcctgaatc aatggacccg tcagagatac
     6721 tggatgacaa atcacattct ttcaccagaa caaggttagc ttcttggctg tcagagaacc
     6781 gaggggggcc tgttcctagc gagaaggtca ttatcacggc cctgtctaag ccacctgtca
     6841 atccccgaga gtttttgaaa tctatagacc tcggaggatt gccagatgag gatttgataa
     6901 ttggcctcaa accaaaggaa cgggagttga agatcgaggg ccgattcttt gctctaatgt
     6961 catggaatct aagattatat tttgtcatca ccgaaaagct cttggccaac tacattttgc
     7021 cactttttga cgcactgact atgacagaca acctgaacaa ggtgttcaaa aagttgatcg
     7081 acagggtcac cgggcaaggg cttttggact attctagggt cacatacgca tttcacctgg
     7141 actatgagaa atggaacaat catcaaagat tggagtcaac agaggatgta ttctctgtcc
     7201 tagatcaggt gtttggattg aagagggtgt tttctagaac acacgagttt tttcagaagt
     7261 cctggatcta ttattcagac agatcagacc tcattgggtt atgggaggac caaatatatt
     7321 gcttggatat gtctaacggc ccaacctgct ggaatggcca agatggcggg ctagagggct
     7381 tgcggcagaa gggctggagt ctagtcagtt tattaatgat agatagagaa tctcaaacca
     7441 ggaacacaag aaccaagata ctagctcaag gagacaacca ggttctgtgt cctacataca
     7501 tgttgtcacc gggattgtct caagaggggc ttctctatga gttagagagc atatcaagga
     7561 atgcactctc aatataccga gctatcgagg aaggagcatc taagctgggg ctgatcatca
     7621 agaaggaaga gaccatgtgt agttatgact ttctcatata tgggaagacc cccttatttc
     7681 gaggcaacat attggtgcct gaatccaaaa gatgggcccg agtctcttgc atctctaacg
     7741 accaaatagt caacctcgcc aatataatgt cgacagtatc caccaatgcg ctgacagtgg
     7801 cacaacactc tcaatctctg atcaaaccta tgagggattt tctgctcatg tcagtacagg
     7861 cagttttcca ctacctgttg tttagcccaa tcctaaaagg cagagtttat aagattctga
     7921 gtgctgaagg ggagagcttt ctcctagcca tgtcgcggat aatctaccta gatccttctt
     7981 tgggaggggt gtctggaatg tctctcggga ggttccatat acgtcagttc tcagaccctg
     8041 tctctgaagg gttgtcattc tggagagaga tctggttagg ctctcatgag tcctggattc
     8101 acgcgttgtg tcaggaggcc gggaaccccg atcttggaga gagaacactc gagagcttca
     8161 ctcgcctttt agaagatcct actaccttaa atatcaaagg aggggccagc cctaccattc
     8221 tactcaagga tgctatcaga aaggctctgt acgacgaggt ggacaaggtg gaaaattcag
     8281 agtttcgaga ggcaatcctg ctgtccaaga cccatagaga taactttata ctctttttaa
     8341 aatctgttga gcctctgttc cctcgatttc tcagtgagct cttcagttcg tctttcttgg
     8401 gaataccgga gtcaatcatt ggactgatac aaaactcccg gacaataaga aggcagttta
     8461 gaaagagtct ctcaagaact ttagaagagt ccttctacaa ctcagagatc cacgggatta
     8521 atcggataac ccagacacct caaagggtcg gaagggtgtg gccttgctct tcagagaggg
     8581 cggatctact tagggagatc tcttggggga ggaaagtggt aggcacgaca gttcctcacc
     8641 cttccgagat gttggagttg tttcccaaat cctccatttc ctgcacttgt ggagcaacag
     8701 ggggaggcaa tcctagagtc tctgtatcag tactcccgtc cttcgatcag tcatttttct
     8761 cacggggccc cctaaaggga tacttgggct cgtccacctc tatgtcaacc cagctattcc
     8821 atgcatggga aaaagtcact aatgttcatg tggtgaaaag ggctctatcg ttaaaagaat
     8881 ctataaactg gttcatcaat aggaattcca atttggctca aactctaatt ggaaacatca
     8941 tgtctctgac aggccctgac ttccctctag aagaggcccc tgttttcaaa cggacagggt
     9001 cagccttgca taggttcaag tctgccagat acagcgaagg agggtattct tctgtttgcc
     9061 ctaaccttct ctcccatatc tctgttagta cagacactat gtctgatttg acccaaaacg
     9121 ggaagaacta tgatttcatg tttcagccat tgatgcttta tgcgcaaaca tggacatcgg
     9181 aactggtaca gagggataca agacttagag actccacgtt tcactggcac cttcggtgca
     9241 acagatgtgt gaggcccatt gatgatataa cactggaaac ttctcagatc ttcgagttcc
     9301 cggatgtgtc aaaaaggata tccaggatgg tttctggagc tgtccctcaa tttcagaagc
     9361 ttcctgatat ccgtctaaga ccaggtgatt ttgaatccct aagtggtaga gaaaagtctc
     9421 gccatatagg gtcagctcag gggctcttat actcaatctt agtagcaatt cacgactcag
     9481 gatacaatga tgggaccatc ttccctgtca acatatacgg caaagtttcc cccagagact
     9541 atttgagagg gcttgcaaga gggatcttga tagggtcctc gatttgcttc ttgacacgaa
     9601 tgacaaatat taacattaac agacctcttg aattgatctc aggggtaatt tcctatattc
     9661 tcctgaggct ggataatcat ccctctctgt atataatgct cagagaaccg tctcttagag
     9721 gagaaatatt ctctatccct cagaaaatcc ccgccgctta cccaaccact atgagagaag
     9781 gcaacagatc gattttgtgt tacctccaac acgtgctacg ctatgagcga gaggcaatca
     9841 cggcgtcccc ggagaatgac tggctgtgga tcttctcaga cttcagaagt gtgaaaatga
     9901 cgtacttgac cctcattact taccagtctc acctcctact ccagagggtt gagagaaact
     9961 tgtctaagag tatgagagct actctgcgac aaatgggttc cttaatgagg caagtgctgg
    10021 gtgggcacgg agaagatacc ttggagtcag acgatgacat tcaacgatta ctaaaagact
    10081 ctttgcgaaa gacaaggtgg gtggatcaag aggtgcgcca tgcagctaga accatgaatg
    10141 gagattacag ccccgacaag aaagtatccc gtaaggtagg atgttcagaa tgggtctgct
    10201 ctgctcaaca gattgccgtc tccacctcag ccaacccggc ccctgtctca gagcttgaca
    10261 ttagggccct ctctaagcgg tttcaaaacc ccttgatctc gggcctgaga gtggttcagt
    10321 gggcaaccgg tgcccattat aagcttaagc ctattctaga tgatctaaat gttttcccat
    10381 ctctctgtct tgtaattgga gacgggtcag ggggaatatc aagggcagtt ctcaacatgt
    10441 ttccagattc taagcttgtg ttcaacagcc tattggaggt gaatgatctg atggcttccg
    10501 gaacacatcc actgcctcct tcagcaatca tgagtggagg agatgacatc atctccagag
    10561 tgatagactt tgactcaata tgggagaaac cgtccgacct gaggaactcg gccacctgga
    10621 gatacttcca gtcggttcaa aaacaggtca acatgtcgta tgacctcatt atttgtgatg
    10681 cagaagttac tgatattgca tctatcaacc ggataactct gttgatgtct gatttcgcat
    10741 tgtctataga tggaccactt tatctggtct tcaaaactta cgggactatg ctagtaaacc
    10801 cggactataa agctattcaa catctgtcaa gagcgttccc ttcggtcaca gggtttgtaa
    10861 cccaagtaac ttcatccttt tcttctgagc tatacctccg gttctctaaa cgaggaaagt
    10921 ttttcaggga cgccgagtac ttgacctctt ccacccttcg agagatgagc cttgtgttgt
    10981 tcaattgcag cagccccaaa agtgagatgc agagagctcg ttccttaaac tatcaagacc
    11041 tggtaagggg atttcctgaa gagatcatat caaatcctta caacgaaatg atcataactc
    11101 tgattgacaa tgatgtagag tccttcctag tccacaagat ggtggatgat cttgagctac
    11161 agaggggaac tctgtctaaa gtggctatca ttatatccat catgatcgtt ttttccaata
    11221 gagtcttcaa catttccaaa cctttgactg accccttgtt ctatccaccg tctgatccta
    11281 aaatcctgag gcacttcaac atatgttgca gtactatgat gtatctatct accgctttag
    11341 gcgacgtccc taacttcgca agacttcatg acctgtataa cagacctata acttgttact
    11401 tcagaaaaca agttattcga gggaatattt atctatcttg gagttggtcc gatgataccc
    11461 cagtgttcaa gagagtagcc tgtaattcta gcttgagtct gtcatctcac tggatcaggt
    11521 tgatctacaa gatagtgaag actaccagac tcattggcag cataaaagac ctatcaggag
    11581 aggtagaacg acatcttcat gggtataaca gatggatcac cctcgaggat atccgatcta
    11641 gatcatccct actagattac agttgtttgt aagccggata ttaccgaaag cctgtgcatg
    11701 ctaaaattct tgtatgatgc atcttgaaaa aaacaagatc ttgaatccgg acctctggtt
    11761 gtttgattgt tttttccatc tttattgttt ttttgttaag cgtgggtcgg catggcatct
    11821 ccacctcctc gcggtccgac ctgggcatcc gaaggaggac gcacgtccac tcggatggct
    11881 aagggagggg cggggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg
    11941 ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt
    12001 ttttgctgaa agtcgcgctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt
    12061 tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt
    12121 gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg
    12181 ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg
    12241 cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg
    12301 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat
    12361 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc
    12421 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc
    12481 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
    12541 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
    12601 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg
    12661 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc
    12721 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg
    12781 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc
    12841 ttgaagtggt ggcctaacta cggctacact agaaggacag tatttggtat ctgcgctctg
    12901 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc
    12961 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct
    13021 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt
    13081 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa
    13141 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa
    13201 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc
    13261 tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct
    13321 gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca
    13381 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt
    13441 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt
    13501 gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc
    13561 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc
    13621 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt
    13681 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact
    13741 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc
    13801 ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt
    13861 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg
    13921 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct
    13981 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa
    14041 tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt
    14101 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc
    14161 acatttcccc gaaaagtgcc acctgacgcg ccctgtagcg gcgcattaag cgcggcgggt
    14221 gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc
    14281 gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg
    14341 gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat
    14401 tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg
    14461 ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct
    14521 atctcggtct attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa
    14581 aatgagctga tttaacaaaa atttaacgcg aattttaaca aaatattaac gcttacaatt
    14641 tccattcgcc attcaggctg cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc
    14701 tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg gtaacgccag
    14761 ggttttccca gtcacgacgt tgtaaaacga cggccagtga gcgcgcctag ttattaatag
    14821 taatcaatta cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt
    14881 acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg
    14941 acgtatgttc ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat
    15001 ttacggtaaa ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct
    15061 attgacgtca atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg
    15121 gactttccta cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg
    15181 ttttggcagt acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc
    15241 caccccattg acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa
    15301 tgtcgtaaca actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc
    15361 tatataagca gagctctctg gctaactaga gaacccactg cttactggct tatcgaaatt
    15421 aatacgactc actataggga gacccaagct ggctagatta agcgtctgat gagtccgtga
    15481 ggacgaaacc cggcgtaccg ggtc
//
