LOCUS       sequence-139567-        1026 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  SapTrap FLP-on C-terminal connector donor plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1026)
  AUTHORS   Schwartz ML, Jorgensen EM
  TITLE     SapTrap, a Toolkit for High-Throughput CRISPR/Cas9 Gene Modification
            in Caenorhabditis elegans.
  JOURNAL   Genetics. 2016 Feb 2. pii: genetics.115.184275.
  PUBMED    26837755
REFERENCE   2  (bases 1 to 1026)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genetics. 
            2016 Feb 2. pii: genetics.115.184275."
COMMENT     Sequence Label: pMLS279
FEATURES             Location/Qualifiers
     source          1..1026
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     27..43
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     28..44
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    103..136
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     protein_bind    548..581
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     primer_bind     complement(615..631)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(615..631)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(668..688)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(668..686)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(707..723)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(707..723)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(825..844)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     primer_bind     complement(903..922)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
ORIGIN
        1 gggcgaattg ggtaccgggc cccccctcga ggtcgacggt atcgataagc ttgatatcga
       61 attcctgcag cccgtacaaa aaagcaggct gctcttcggc gggaagttcc tattctctag
      121 aaagtatagg aacttcataa attttcaaat tttaaatact gaatatttgt tttttttcct
      181 attatttatt tattctcttt gtgttttttt tcttgctttc taaaaaatta attcaatcca
      241 aatctaaaca tttttttttc tctttccgtc tcccaattcg tattccgctc ctctcatctg
      301 aacacaatgt gcaagtttat ttatcttctc gctttcattt cattaggacg tggggggaat
      361 tggtggaagg gggaaacaca caaaaggatg atggaaatga aataaggaca cacaatatgc
      421 aacaacattc aattcagaaa tatggaggaa ggtttaaaag aaaacataaa aatatataga
      481 ggaggaagga aaactagtaa aaaataagca aagaaattag gcgaacgatg agaattgtcc
      541 tcgcttggaa gttcctattc tctagaaagt ataggaactt caatgggaag agcacccagc
      601 tttcttgtac gggggatcca ctagttctag agcggccgcc accgcggtgg agctccagct
      661 tttgttccct ttagtgaggg ttaattgcgc gcttggcgta atcatggtca tagctgtttc
      721 ctggcagctc tggcccgtgt ctcaaaatct ctgatgttac attgcacaag ataaaaatat
      781 atcatcatga acaataaaac tgtctgctta cataaacagt aatacaaggg gtgttatgag
      841 ccatattcaa cgggaaacgt cgaggccgcg attaaattcc aacatggatg ctgatttata
      901 tgggtataaa tgggctcgcg ataatgtcgg gcaatcaggt gcgacaatct atcgcttgta
      961 tgggaagccc gatgcgccag agttgtttct gaaacatggc aaaggtagcg ttgccaatga
     1021 tgttac
//
