LOCUS       sequence-139568-         919 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  SapTrap FLP-on C-terminal connector donor plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 919)
  AUTHORS   Schwartz ML, Jorgensen EM
  TITLE     SapTrap, a Toolkit for High-Throughput CRISPR/Cas9 Gene Modification
            in Caenorhabditis elegans.
  JOURNAL   Genetics. 2016 Feb 2. pii: genetics.115.184275.
  PUBMED    26837755
REFERENCE   2  (bases 1 to 919)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genetics. 
            2016 Feb 2. pii: genetics.115.184275."
COMMENT     Sequence Label: pMLS279
FEATURES             Location/Qualifiers
     source          1..919
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     54..70
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     54..70
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     protein_bind    complement(104..137)
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     protein_bind    complement(549..582)
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     primer_bind     complement(641..657)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(642..658)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     complement(682..701)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(683..701)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(711..728)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(711..727)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(720..742)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     terminator      791..877
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ttaaccctca ctaaagggaa caaaagctgg agctccaccg cggtggcggc cgctctagaa
       61 ctagtggatc ccccgtacaa gaaagctggg tgctcttccc attgaagttc ctatactttc
      121 tagagaatag gaacttccaa gcgaggacaa ttctcatcgt tcgcctaatt tctttgctta
      181 ttttttacta gttttccttc ctcctctata tatttttatg ttttctttta aaccttcctc
      241 catatttctg aattgaatgt tgttgcatat tgtgtgtcct tatttcattt ccatcatcct
      301 tttgtgtgtt tcccccttcc accaattccc cccacgtcct aatgaaatga aagcgagaag
      361 ataaataaac ttgcacattg tgttcagatg agaggagcgg aatacgaatt gggagacgga
      421 aagagaaaaa aaaatgttta gatttggatt gaattaattt tttagaaagc aagaaaaaaa
      481 acacaaagag aataaataaa taataggaaa aaaaacaaat attcagtatt taaaatttga
      541 aaatttatga agttcctata ctttctagag aataggaact tcccgccgaa gagcagcctg
      601 cttttttgta cgggctgcag gaattcgata tcaagcttat cgataccgtc gacctcgagg
      661 gggggcccgg tacccaattc gccctatagt gagtcgtatt acgcgcgctc actggccgtc
      721 gttttacaac gtcgtgactg ggaaaacatc catgctagcg ttaacgcgag agtagggaac
      781 tgccaggcat caaataaaac gaaaggctca gtcggaagac tgggcctttc gttttatctg
      841 ttgtttgtcg gtgaacgctc tcctgagtag gacaaatccg ccgggagcgg atttgaacgt
      901 tgtgaagcaa cggcccgga
//
