LOCUS       sequence-139571-         897 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes the G protein to enable transsynaptic RAV infection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 897)
  AUTHORS   Reardon TR, Murray AJ, Turi GF, Wirblich C, Croce KR, Schnell MJ, 
            Jessell TM, Losonczy A
  TITLE     Rabies Virus CVS-N2c Strain Enhances Retrograde Synaptic Transfer 
            and Neuronal Viability.
  JOURNAL   Neuron. 2016 Jan 19. pii: S0896-6273(16)00005-2. doi: 
            10.1016/j.neuron.2016.01.004.
  PUBMED    26804990
REFERENCE   2  (bases 1 to 897)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Neuron. 
            2016 Jan 19. pii: S0896-6273(16)00005-2. doi: 
            10.1016/j.neuron.2016.01.004."
COMMENT     Sequence Label: DIO-EF1a-FLEX-H2B-3XHA-P2A-N2c(G)
FEATURES             Location/Qualifiers
     source          1..897
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(45..78)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    complement(129..162)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     CDS             178..555
                     /codon_start=1
                     /gene="HIST1H2BJ"
                     /product="human histone H2B"
                     /label=H2B
                     /translation="MPEPAKSAPAPKKGSKKAVTKAQKKGGKKRKRSRKESYSIYVYKV
                     LKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLP
                     GELAKHAVSEGTKAITKYTSSK"
     CDS             574..663
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
     CDS             673..729
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
ORIGIN
        1 ttgtaatcca gaggttgatt atcgataagc ttgatatcga attcataact tcgtatagca
       61 tacattatac gaagttattc tttgcaccat tctaaagaat aacagtgata atttctgggt
      121 taaggcaaat aacttcgtat aggatacttt atacgaagtt atgctagagc cgccaccatg
      181 ccagagccag cgaagtctgc tcccgccccg aaaaagggct ccaagaaggc ggtgactaag
      241 gcgcagaaga aaggcggcaa gaagcgcaag cgcagccgca aggagagcta ttccatctat
      301 gtgtacaagg ttctgaagca ggtccaccct gacaccggca tttcgtccaa ggccatgggc
      361 atcatgaact cgtttgtgaa cgacattttc gagcgcatcg caggtgaggc ttcccgcctg
      421 gcgcattaca acaagcgctc gaccatcacc tccagggaga tccagacggc cgtgcgcctg
      481 ctgctgcctg gggagttggc caagcacgcc gtgtccgagg gtactaaggc catcaccaag
      541 tacaccagct ctaaggatgg atccggcgcc gtttacccat acgatgttcc tgactatgcg
      601 ggctatccct atgacgtccc ggactatgca ggatcctatc catatgacgt tccagattac
      661 gctggaagcg gagctactaa cttcagcctg ctgaagcagg ctggagacgt ggaggagaac
      721 cctggaccta tggttcctca ggttcttttg tttgtactcc ttctgggttt ttcgttgtgt
      781 ttcgggaagt tccccattta cacgatacca gacgaacttg gtccctggag ccctattgac
      841 atacaccatc tcagctgtcc aaataacctg gttgtggagg atgaaggatg taccaac
//
