LOCUS       sequence-139572-        1081 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  SapTrap flexible linker C-terminal connector donor plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1081)
  AUTHORS   Schwartz ML, Jorgensen EM
  TITLE     SapTrap, a Toolkit for High-Throughput CRISPR/Cas9 Gene Modification
            in Caenorhabditis elegans.
  JOURNAL   Genetics. 2016 Feb 2. pii: genetics.115.184275.
  PUBMED    26837755
REFERENCE   2  (bases 1 to 1081)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genetics. 
            2016 Feb 2. pii: genetics.115.184275."
COMMENT     Sequence Label: pMLS287
FEATURES             Location/Qualifiers
     source          1..1081
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     27..43
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     28..44
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(136..152)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(136..152)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(189..209)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(189..207)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(228..244)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(228..244)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(346..365)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     primer_bind     complement(424..443)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
ORIGIN
        1 gggcgaattg ggtaccgggc cccccctcga ggtcgacggt atcgataagc ttgatatcga
       61 attcctgcag cccgctcttc ggcgggcagc ggtggcagtg gaggtaccgg cggaagcggt
      121 atgcgaagag cgggggatcc actagttcta gagcggccgc caccgcggtg gagctccagc
      181 ttttgttccc tttagtgagg gttaattgcg cgcttggcgt aatcatggtc atagctgttt
      241 cctggcagct ctggcccgtg tctcaaaatc tctgatgtta cattgcacaa gataaaaata
      301 tatcatcatg aacaataaaa ctgtctgctt acataaacag taatacaagg ggtgttatga
      361 gccatattca acgggaaacg tcgaggccgc gattaaattc caacatggat gctgatttat
      421 atgggtataa atgggctcgc gataatgtcg ggcaatcagg tgcgacaatc tatcgcttgt
      481 atgggaagcc cgatgcgcca gagttgtttc tgaaacatgg caaaggtagc gttgccaatg
      541 atgttacaga tgagatggtc agactaaact ggctgacgga atttatgcct cttccgacca
      601 tcaagcattt tatccgtact cctgatgatg catggttact caccactgcg atccccggaa
      661 aaacagcatt ccaggtatta gaagaatatc ctgattcagg tgaaaatatt gttgatgcgc
      721 tggcagtgtt cctgcgccgg ttgcattcga ttcctgtttg taattgtcct tttaacagcg
      781 atcgcgtatt tcgtctcgct caggcgcaat cacgaatgaa taacggtttg gttgatgcga
      841 gtgattttga tgacgagcgt aatggctggc ctgttgaaca agtctggaaa gaaatgcata
      901 aacttttgcc attctcaccg gattcagtcg tcactcatgg tgatttctca cttgataacc
      961 ttatttttga cgaggggaaa ttaataggtt gtattgatgt tggacgagtc ggaatcgcag
     1021 accgatacca ggatcttgcc atcctatgga actgcctcgg tgagttttct ccttcattac
     1081 a
//
