LOCUS       sequence-139573-         745 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  SapTrap flexible linker C-terminal connector donor plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 745)
  AUTHORS   Schwartz ML, Jorgensen EM
  TITLE     SapTrap, a Toolkit for High-Throughput CRISPR/Cas9 Gene Modification
            in Caenorhabditis elegans.
  JOURNAL   Genetics. 2016 Feb 2. pii: genetics.115.184275.
  PUBMED    26837755
REFERENCE   2  (bases 1 to 745)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genetics. 
            2016 Feb 2. pii: genetics.115.184275."
COMMENT     Sequence Label: pMLS287
FEATURES             Location/Qualifiers
     source          1..745
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     51..67
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     51..67
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(159..175)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(160..176)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     complement(200..219)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(201..219)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(229..246)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(229..245)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(238..260)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     terminator      309..395
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      487..514
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     primer_bind     complement(673..690)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 accctcacta aagggaacaa aagctggagc tccaccgcgg tggcggccgc tctagaacta
       61 gtggatcccc cgctcttcgc ataccgcttc cgccggtacc tccactgcca ccgctgcccg
      121 ccgaagagcg ggctgcagga attcgatatc aagcttatcg ataccgtcga cctcgagggg
      181 gggcccggta cccaattcgc cctatagtga gtcgtattac gcgcgctcac tggccgtcgt
      241 tttacaacgt cgtgactggg aaaacatcca tgctagcgtt aacgcgagag tagggaactg
      301 ccaggcatca aataaaacga aaggctcagt cggaagactg ggcctttcgt tttatctgtt
      361 gtttgtcggt gaacgctctc ctgagtagga caaatccgcc gggagcggat ttgaacgttg
      421 tgaagcaacg gcccggaggg tggcgggcag gacgcccgcc ataaactgcc aggcatcaaa
      481 ctaagcagaa ggccatcctg acggatggcc tttttgcgtt tctacaaact cttcctggct
      541 agcggtacgc gtattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc
      601 gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg
      661 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
      721 atcagctcac tcaaaggcgg taata
//
