LOCUS       sequence-139576-         997 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  SapTrap Flagtag-TEV C-terminal connector donor plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 997)
  AUTHORS   Schwartz ML, Jorgensen EM
  TITLE     SapTrap, a Toolkit for High-Throughput CRISPR/Cas9 Gene Modification
            in Caenorhabditis elegans.
  JOURNAL   Genetics. 2016 Feb 2. pii: genetics.115.184275.
  PUBMED    26837755
REFERENCE   2  (bases 1 to 997)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genetics. 
            2016 Feb 2. pii: genetics.115.184275."
COMMENT     Sequence Label: pMLS382
FEATURES             Location/Qualifiers
     source          1..997
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     27..43
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     28..44
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             105..128
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             141..161
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     primer_bind     complement(203..219)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(203..219)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(256..276)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(256..274)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(295..311)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(295..311)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(413..432)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     primer_bind     complement(491..510)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
ORIGIN
        1 gggcgaattg ggtaccgggc cccccctcga ggtcgacggt atcgataagc ttgatctgct
       61 cttcggcgag tactagcggt ggcagtggag gtaccggcgg aagcgactac aaggacgacg
      121 acgacaagga gccaaccact gagaatctgt actttcagag cagtggcatg ggaagagcac
      181 atcgaattcc tgcagcccgg gggatccact agttctagag cggccgccac cgcggtggag
      241 ctccagcttt tgttcccttt agtgagggtt aattgcgcgc ttggcgtaat catggtcata
      301 gctgtttcct ggcagctctg gcccgtgtct caaaatctct gatgttacat tgcacaagat
      361 aaaaatatat catcatgaac aataaaactg tctgcttaca taaacagtaa tacaaggggt
      421 gttatgagcc atattcaacg ggaaacgtcg aggccgcgat taaattccaa catggatgct
      481 gatttatatg ggtataaatg ggctcgcgat aatgtcgggc aatcaggtgc gacaatctat
      541 cgcttgtatg ggaagcccga tgcgccagag ttgtttctga aacatggcaa aggtagcgtt
      601 gccaatgatg ttacagatga gatggtcaga ctaaactggc tgacggaatt tatgcctctt
      661 ccgaccatca agcattttat ccgtactcct gatgatgcat ggttactcac cactgcgatc
      721 cccggaaaaa cagcattcca ggtattagaa gaatatcctg attcaggtga aaatattgtt
      781 gatgcgctgg cagtgttcct gcgccggttg cattcgattc ctgtttgtaa ttgtcctttt
      841 aacagcgatc gcgtatttcg tctcgctcag gcgcaatcac gaatgaataa cggtttggtt
      901 gatgcgagtg attttgatga cgagcgtaat ggctggcctg ttgaacaagt ctggaaagaa
      961 atgcataaac ttttgccatt ctcaccggat tcagtcg
//
