LOCUS       sequence-139577-         713 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  SapTrap Flagtag-TEV C-terminal connector donor plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 713)
  AUTHORS   Schwartz ML, Jorgensen EM
  TITLE     SapTrap, a Toolkit for High-Throughput CRISPR/Cas9 Gene Modification
            in Caenorhabditis elegans.
  JOURNAL   Genetics. 2016 Feb 2. pii: genetics.115.184275.
  PUBMED    26837755
REFERENCE   2  (bases 1 to 713)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genetics. 
            2016 Feb 2. pii: genetics.115.184275."
COMMENT     Sequence Label: pMLS382
FEATURES             Location/Qualifiers
     source          1..713
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     54..70
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     54..70
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     CDS             complement(112..132)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             complement(145..168)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     complement(229..245)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(230..246)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     complement(270..289)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(271..289)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(299..316)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(299..315)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(308..330)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     terminator      379..465
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      557..584
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ttaaccctca ctaaagggaa caaaagctgg agctccaccg cggtggcggc cgctctagaa
       61 ctagtggatc ccccgggctg caggaattcg atgtgctctt cccatgccac tgctctgaaa
      121 gtacagattc tcagtggttg gctccttgtc gtcgtcgtcc ttgtagtcgc ttccgccggt
      181 acctccactg ccaccgctag tactcgccga agagcagatc aagcttatcg ataccgtcga
      241 cctcgagggg gggcccggta cccaattcgc cctatagtga gtcgtattac gcgcgctcac
      301 tggccgtcgt tttacaacgt cgtgactggg aaaacatcca tgctagcgtt aacgcgagag
      361 tagggaactg ccaggcatca aataaaacga aaggctcagt cggaagactg ggcctttcgt
      421 tttatctgtt gtttgtcggt gaacgctctc ctgagtagga caaatccgcc gggagcggat
      481 ttgaacgttg tgaagcaacg gcccggaggg tggcgggcag gacgcccgcc ataaactgcc
      541 aggcatcaaa ctaagcagaa ggccatcctg acggatggcc tttttgcgtt tctacaaact
      601 cttcctggct agcggtacgc gtattaattg cgttgcgctc actgcccgct ttccagtcgg
      661 gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggc
//
