LOCUS       sequence-139582-        2751 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cloning vector containing N2c Glycoprotein and histone GFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2751)
  AUTHORS   Reardon TR, Murray AJ, Turi GF, Wirblich C, Croce KR, Schnell MJ, 
            Jessell TM, Losonczy A
  TITLE     Rabies Virus CVS-N2c Strain Enhances Retrograde Synaptic Transfer 
            and Neuronal Viability.
  JOURNAL   Neuron. 2016 Jan 19. pii: S0896-6273(16)00005-2. doi: 
            10.1016/j.neuron.2016.01.004.
  PUBMED    26804990
REFERENCE   2  (bases 1 to 2751)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Neuron. 
            2016 Jan 19. pii: S0896-6273(16)00005-2. doi: 
            10.1016/j.neuron.2016.01.004."
COMMENT     Sequence Label: pcDNA-H2B-EGFP-P2A-N2c(G)
FEATURES             Location/Qualifiers
     source          1..2751
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1..375
                     /codon_start=1
                     /gene="HIST1H2BJ"
                     /product="human histone H2B"
                     /label=H2B
                     /translation="PEPAKSAPAPKKGSKKAVTKAQKKGGKKRKRSRKESYSIYVYKVL
                     KQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLPG
                     ELAKHAVSEGTKAITKYTSAK"
     regulatory      388..397
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(394..396,397..399,400..1110)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(394..396,397..399,400..1110)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(439..460)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(700..719)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1047..1068
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             1120..1176
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
ORIGIN
        1 ccagagccag cgaagtctgc tcccgccccg aaaaagggct ccaagaaggc ggtgactaag
       61 gcgcagaaga aaggcggcaa gaagcgcaag cgcagccgca aggagagcta ttccatctat
      121 gtgtacaagg ttctgaagca ggtccaccct gacaccggca tttcgtccaa ggccatgggc
      181 atcatgaatt cgtttgtgaa cgacattttc gagcgcatcg caggtgaggc ttcccgcctg
      241 gcgcattaca acaagcgctc gaccatcacc tccagggaga tccagacggc cgtgcgcctg
      301 ctgctgcctg gggagttggc caagcacgcc gtgtccgagg gtactaaggc catcaccaag
      361 tacaccagcg ctaaggatcc accggtcgcc accatggtga gcaagggcga ggagctgttc
      421 accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca caagttcagc
      481 gtgtccggcg agggcgaggg cgatgccacc tacggcaagc tgaccctgaa gttcatctgc
      541 accaccggca agctgcccgt gccctggccc accctcgtga ccaccctgac ctacggcgtg
      601 cagtgcttca gccgctaccc cgaccacatg aagcagcacg acttcttcaa gtccgccatg
      661 cccgaaggct acgtccagga gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc
      721 cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct gaagggcatc
      781 gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaacta caacagccac
      841 aacgtctata tcatggccga caagcagaag aacggcatca aggtgaactt caagatccgc
      901 cacaacatcg aggacggcag cgtgcagctc gccgaccact accagcagaa cacccccatc
      961 ggcgacggcc ccgtgctgct gcccgacaac cactacctga gcacccagtc cgccctgagc
     1021 aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac cgccgccggg
     1081 atcactctcg gcatggacga gctgtacaag ggaagcggag ctactaactt cagcctgctg
     1141 aagcaggctg gagacgtgga ggagaaccct ggacctatgg ttcctcaggt tcttttgttt
     1201 gtactccttc tgggtttttc gttgtgtttc gggaagttcc ccatttacac gataccagac
     1261 gaacttggtc cctggagccc tattgacata caccatctca gctgtccaaa taacctggtt
     1321 gtggaggatg aaggatgtac caacctgtcc gagttctcct acatggaact caaagtggga
     1381 tacatctcag ccatcaaagt gaacgggttc acttgcacag gtgttgtgac agaggcagag
     1441 acctacacca actttgttgg ttatgtcaca accacattca agagaaagca tttccgcccc
     1501 accccagacg catgtagagc cgcgtataac tggaagatgg ccggtgaccc cagatatgaa
     1561 gagtccctac acaatccata ccccgactac cactggcttc gaactgtaag aaccaccaaa
     1621 gagtccctca ttatcatatc cccaagtgtg acagatttgg acccatatga caaatccctt
     1681 cactcaaggg tcttccctgg cggaaagtgc tcaggaataa cggtgtcctc tacctactgc
     1741 tcaactaacc atgattacac catttggatg cccgagaatc cgagaccaag gacaccttgt
     1801 gacattttta ccaatagcag agggaagaga gcatccaacg ggaacaagac ttgcggcttt
     1861 gtggatgaaa gaggcctgta taagtctcta aaaggagcat gcaggctcaa gttatgtgga
     1921 gttcttggac ttagacttat ggatggaaca tgggtcgcga tgcaaacatc agatgagacc
     1981 aaatggtgcc ctccagatca gttggtgaat ttgcacgact ttcgctcaga cgagattgag
     2041 catctcgttg tggaggagtt agtcaagaaa agagaggaat gtctggacgc attagagtcc
     2101 atcatgacca ccaagtcagt aagtttcaga cgtctcagtc acctgagaaa acttgtccca
     2161 gggtttggaa aagcatatac catattcaac aaaaccttga tggaggctga tgctcactac
     2221 aagtcagtcc ggacctggaa tgagatcatc ccctcaaaag ggtgtttgaa agttggagga
     2281 aggtgccatc ctcatgtgaa cggggtgttt ttcaatggta taatattagg gcctgacgac
     2341 catgtcctaa tcccagagat gcaatcatcc ctcctccagc aacatatgga gttgttggaa
     2401 tcttcagtta tccccctgat gcaccccctg gcagaccctt ctacagtttt caaagaaggt
     2461 gatgaggctg aggattttgt tgaagttcac ctccccgatg tgtacaaaca gatctcaggg
     2521 gttgacctgg gtctcccgaa ctggggaaag tatgtattga tgactgcagg ggccatgatt
     2581 ggcctggtgt tgatattttc cctaatgaca tggtgcagaa gagccaatcg accagaatcg
     2641 aaacaacgca gttttggagg gacagggggg aatgtgtcag tcacttccca aagcggaaaa
     2701 gtcatacctt catgggaatc atataagagt ggaggtgaga tcagactgtg a
//
