LOCUS       sequence-139585-         938 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  SapTrap syntron-embedded FLP-on N-terminal connector donor plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 938)
  AUTHORS   Schwartz ML, Jorgensen EM
  TITLE     SapTrap, a Toolkit for High-Throughput CRISPR/Cas9 Gene Modification
            in Caenorhabditis elegans.
  JOURNAL   Genetics. 2016 Feb 2. pii: genetics.115.184275.
  PUBMED    26837755
REFERENCE   2  (bases 1 to 938)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genetics. 
            2016 Feb 2. pii: genetics.115.184275."
COMMENT     Sequence Label: pMLS281
FEATURES             Location/Qualifiers
     source          1..938
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    66..90
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    133..166
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     protein_bind    564..597
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     protein_bind    complement(671..770)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(787..806)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(788..806)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(811..827)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(811..827)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 ttttattttg actgatagtg acctgttcgt tgcaacaaat tgatgagcaa tgctttttta
       61 taatgccaac tttgtacaaa aaagcaggct atgctcttcg ggttcggtaa gtttaaacat
      121 gattttacta acgaagttcc tattctctag aaagtatagg aacttctaac taatctgatt
      181 taaattttca gggtagccat ggcttcccgc cggaggtgga ggagcaggat gatggcacgc
      241 tgcccatgtc ttgtgcccag gagagcggga tggaccgtca ccctgcagcc tgtgcttctg
      301 ctaggatcaa tgtgtaagct gtctcatcct actttcacct agttaactgc ttgtcttaaa
      361 atctatgctt ctctttagta tctaaaattt tcctagaagc ttacaagtat ataaatggtc
      421 tcttctcaat aaaggttgta tatttattca tcttattgaa tctgccattt cctcgttttt
      481 gcgagtttat ataccttcca attttctttc tattgtattt tcaacttcta attttaattc
      541 agggaaactg cttcaacgca tcagaagttc ctattctcta gaaagtatag gaacttctaa
      601 ctaatctgat ttaaattttc agggtatggg cagcggaggt accggcggta gtggaggcac
      661 gcgaagagct acccagcttt cttgtacaaa gttggcatta taagaaagca ttgcttatca
      721 atttgttgca acgaacaggt cactatcagt caaaataaaa tcattatttg ccatccagct
      781 gatatcccct atagtgagtc gtattacatg gtcatagctg tttcctggca gctctggccc
      841 gtgtctcaaa atctctgatg ttacattgca caagataaaa atatatcatc atgaacaata
      901 aaactgtctg cttacataaa cagtaataca agggggtg
//
